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2008, Número 2

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Bioquimia 2008; 33 (2)


Caracterización y frecuencia del gen vapD en Helicobacter pylori con diferentes genotipos de vacA y cag-PAI

Morales-Espinosa R, González-Valencia G, Delgado G, Méndez JL, Torres J, Cravioto A

Idioma: Ingles.
Referencias bibliográficas: 38
Paginas: 43-50
Archivo PDF: 173.33 Kb.


RESUMEN

Diversos estudios, realizados con diferentes técnicas de tipificación molecular han demostrado que el contenido genético y arreglo cromosómico entre cepas de Helicobacter pylori son altamente variables. Además, se ha observado esta variabilidad en diferentes genes, como vacA que presenta una estructura de mosaico y cag-PAI, que presenta una organización diferente en el cromosoma. Un estudio preliminar sugiere que el gen vapD, es un gen variable de cepa, presente en aproximadamente 60% de las cepas de H. pylori. El gen vapD está relacionado con el gen de la proteína D asociada a virulencia de Dichelobacter nodosus. El objetivo de este estudio fue caracterizar y determinar la frecuencia del gen vapD en 301 cepas de H. pylori con diferentes genotipos de vacA y cag-PAI, provenientes de 27 pacientes con diferentes patologías gástricas. Los resultados mostraron que el 70% de los pacientes presentan infección múltiple con cepas de H. pylori de diferentes genotipos de vacA y cag-PAI. El 52% de ellos estuvieron colonizados con cepas vapD positivas y vapD negativas. La caracterización de vapD en cepas individuales, mostró una frecuencia del 38%. Este es el primer estudio que muestra la frecuencia de vapD en cepas de H. pylori aisladas de pacientes mexicanos. Con respecto a los genes vacA y cag-PAI, el genotipo más frecuente entre las cepas fue s1-m1/cag-PAI+, aisladas de pacientes con dolor abdominal crónico, úlcera gástrica y úlcera duodenal. El gen vapD no tiene asociación significativa con un alelo específico de vacA o el genotipo de cag-PAI y la patología gástrica.


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