Neurological effects by occlusion of the carotid artery and induced hypoxia in newborn rats.

Jimena Quinzaños-Fresnedo; Roberto Coronado-Zarco; Emilio Arch-Tirado; Antonio Verduzco-Mendoza; Griselda Del Valle-Cabrera; Alfonso Alfaro-Rodríguez

2008, Number 2

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Cir Cir 2008; 76 (2)

Neurological effects by occlusion of the carotid artery and induced hypoxia in newborn rats.

Quinzaños-Fresnedo J, Coronado-Zarco R, Arch-Tirado E, Verduzco-Mendoza A, Valle-Cabrera G, Alfaro-Rodríguez A

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Language: Spanish
References: 45
Page: 119-125
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ABSTRACT

Background: Hypoxic-ischemic encephalopathy is a cause of disability in the infantile population. One of the most used animal models in the hypoxic-ischemic encephalopathy in immature brain is the preparation of Levine applied by Rice in newborn rats and consists of the bond of the left common carotid artery followed by induced hypoxia. The objective of this investigation was to study the neurological effects of the bond of the left common carotid and induced hypoxia in newborn rats.
Methods: Five control rats, five sham rats and five rats with hypoxic-ischemic lesion by means of the application of Levine’s preparation at 7 days of age were used. On day 42 all rats were evaluated by means of the time of grasping, the test of the posterior reflex and analysis of the spontaneous locomotor activity (number of biped stations, number of stepped stalls, grooming time).
Results: The group with lesions presented less grasping time, less number of positive responses to the posterior reflex and lower number of stepped stalls (p = 0.024, 0.002 and 0.0001, respectively). There were no statistically significant differences in grooming time or number of biped stations.
Conclusion: Newborn rats in whom Levine preparation was applied presented clinical alterations that may resemble some of signs that accompany infantile cerebral palsy (grasp problems, wrong answer to postural reflexes and alteration in locomotion).

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