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1998, Number 2

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Vet Mex 1998; 29 (2)

Embryo transfer in recipient ewes treated with growth hormone. Effects on embryo viability

Ramón UJP, Folch PJ, Cocero MJ, Fernández-Arias A, Alabart JL, Garbayo JM

Language: English/Spanish
References: 36
Page: 137-145
PDF size: 668.13 Kb.


Text Extraction

In order to study the survival of embryos transferred to recipient ewes synchronized with FGA and PMSG, and treated with GHp (Growth Hormone porcine), two experiments were done. In experiment 1, 69 Rasa Aragonesa ewes were used (12 donors and 57 recipients). An embryo, morphologically classified as viable, was transferred to each recipient, which was either treated with GHp (R1) or untreated (R2, control group). No effect was observed on the estrous average time. However, the Ovulation Rate (OR) was higher (P‹0.01) in R1. On the other hand, R1 ewes showed higher levels of progesterone (P4), although this increment was not associated with the increase of Ovulation Rate (OR) but with the exogenous application of GHp. Nevertheless, a high correlation (P‹0.01) between the concentration of P4, 4 days and 17 days after fertilization, was found. No differences in fertility were observed between treatments. In experiment 2,40 one-year old Rasa Aragonesa ewes (14 donors and 18 recipients) were used. Ninety-five 2-day old embryos were obtained. Sixty-eight morphologically classified as viable embryos were distributed in groups (8-10 embryos per group) and transferred to 4 recipients treated with GHp (R1) and to 4 untreated recipients (R2). These same embryos were recovered 3 days later from uterus, and their morphologic viability was evaluated. An increase (P‹0.05) in the OR of R1 was observed. No differences in the levels of P4 were detected on the 7th day following treatment with GHp, although the number of data was insufficient to establish differences. Embryo recovery was higher in R1. A slight tendency (P‹0.2) towards a higher quality of embryos from R1 ewes was detected. Data presented in this study lead to the following conclusions: a) in recipient ewes treated with FGA and PMSG, the application of GH produces an increase of P4 plasma concentration 4 days after sponge withdrawal and an increase in OR and b) fertility of transferred embryos is not altered by treating the recipient ewes with GH.


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