Mezo-Menéndez M, Sánchez-Andrade R, Martínez-Santiago JL, Díez-Baños N, Díez-Baños P, Morrondo PP

Language: English/Spanish
References: 20
Page: 75-81
PDF size: 633.90 Kb.

ABSTRACT

IgG specific antibody evolution was studied in experimentally infected and reinfected cattle by indirect ELISA with F. hepatica excretion-secretion antigen. Likewise, the utility of the test to evaluate the different treatment model and the detection of naturally acquired F. hepatica was assessed. Antibody levels rose progressively from the 8th to the 24th week after primoinfection. From the 8th week after primoinfection, 85.7% of the animals were positive by ELISA, which means 4 weeks before the coprological diagnosis. Antibody rate increased in reinfected cattle in the 4th week postreinfection. Sera were positive during the 24 weeks post-reinfection in parasited cattle in the moment of reinfection. In cattle in which primoinfection had been eliminated with an anthelmintic treatment, sera were positive from 4 to 24 weeks after reinfection. It was proved in the necropsy that reinfected animals harboured adult fasciolas, confirming the results obtained by ELISA. The treatment applied 12 weeks after primoinfection was completely effective, however, 42.8% of the sera carried on to be positive during 6 weeks after the treatment. Taking into account this period, ELISA allowed to evaluate in a early and reliable way the efficacy of the treatment applied in the prepatent period (3 weeks after infection). Ten Friesian heifers without F. hepatica infection were kept in pastures with L. truncatula and F. hepatica metacercariae to study the evolution of antibody levels in natural infection. Every animal showed positive absorbance after being 3 months in these pastures, i.e. 5 months earlier than the egg output in faeces.

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