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1999, Number 4

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Vet Mex 1999; 30 (4)

Activity of interferon in splenic lymphocytes chickens during Eimeria tenella primed infection

Juárez EMA, Hernández VX, Téllez IG

Language: English/Spanish
References: 54
Page: 297-305
PDF size: 124.33 Kb.


ABSTRACT

Two studies were carried out to evaluate the interferon (IFN) secretion pattern during primed experimental infection with Eimeria tenella. In the first experiment, the primary response of splenic lymphocytes T (LT) was evaluated in 20 day old broiler chickens (Hubbard x Hubbard) challenged with 1 x 104 E. tenella sporulated oocysts. Nylon wool columns were used to isolate LT in four spleen pools on days 1, 3, 5, 7 and 9 post-infection (pi). In the second experiment, the primary response was also evaluated in LT isolated from individual spleens using Ficoll Hypaque gradients. Challenged and control bird spleen pools were prepared separately. In order to detect the presence of IFN, viruses of Newcastle disease and infectious bursal disease were used in antiviral assays. In the first experiment on day 5 pi, IFN levels in the challenged group reached a different peak in contrast to the control group, it decreased on day 7 pi, and was increased again on day 9 pi (P‹ 0.05). Outputs of the second experiment resembled the first one but reached higher levels. The IFN secretion pattern of in vitro stimulated LT after challenge with E. tenella was different to the secretion pattern of in vitro stimulated LT without challenge with E. tenella. Antiviral assays detected qualitatively and quantitatively avian IFN secreted by in vitro stimulated LT. IFN secretion patterns detected with different LT isolation techniques after the primary infection had a similar performance.


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