Garza-Carbajal A

Language: Spanish
References: 54
Page: 131-135
PDF size: 78.86 Kb.

ABSTRACT

Background. Acid sensing ion channels (ASIC) are ionic channels activated by transient pH reductions in the extracellular environment. Although the activation mechanism is not fully elucidated, it is clear that the channel is activated by proton binding to its extracellular domain, a process that is modulated by calcium and zinc.
Objective. The fact that divalent cations are able to modify ASIC operation, lead us to consider if lead, another divalent cation and widely distributed neurotoxicant, is also capable to affect ASIC function.
Methods. For this purpose, we recorded ASIC currents in rat dorsal root ganglion neurons using the whole cell patch-clamp technique.
Results. The results indicated that lead inhibits ASIC currents in a concentration-dependent fashion.
Conclusions. These results contribute to the understanding of the activation mechanism of ASIC and to explain some of the toxic mechanisms of lead in the organism.

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