Cueto C, García D, Garcés F, Cruz J

Language: English
References: 16
Page: 11-17
PDF size: 117.90 Kb.

ABSTRACT

Suero costeño is a fermented milk product from the Colombian Atlantic coast, which is produced by the spontaneous acidification of raw milk due to the action of environmental microbes during traditional and semi-industrial processes. Eleven fermentations were carried out in experimental settings replicating traditional conditions and changes in concentration among microbial groups involved during the process (Aerobic Mesophilic bacteria, yeasts, Enterobacteriaceae and Lactic Acid Bacteria (LAB)). LAB plays an important role in the fermentation process, especially during the final stage (24 hours). In addition, yeasts seem to have an effect on fermentation, showing an increase during the first hours of the process, while Enterobacterial counts decreased during fermentation. Thirty six LAB strains were isolated from commercial samples and thirty two were identified using the API 50 CH kit (BioMérieux). 41% of the strains identified belonged to the species Lb. plantarum, and 19% were Lb. paracasei subsp. paracasei. Sugars fermented by LAB include milk carbohydrates such as D-Lactose, D-Glucose and D-Galactose. Because of their capacity to use other carbohydrates (manose, celobiose, maltose, fructose, ribose, trehalose, salicin, gentiobiose), it would also be possible to use these strains as starter cultures for other fermentations.

REFERENCES

1. Ben Omar, N. and Ampem, F. (2000) Microbial Community Dynamics during Production of the Mexican Fermented Maize Dough Pozol. Applied and Environmental Microbiology. Vol.66: 3664-3673.

2. De Man, J., Rogosa, M. and Sharpe, M. (1960) A medium for the cultivation ob Lactobacilli. J. Appl.Bact: 130-135.

3. Díaz-Ruiz, G., Guyot, J. P., Ruiz-Teran, F., Morlon-Guyot, J. and Wacher, C. (2003) Microbial and Physiological Characterization of Weakly Amylolytic but Fast-Growing Lactic Acid Bacteria: a Functional Role in Supporting Microbial Diversity in Pozol, a Mexican Fermented Maize Beverage. Applied and Environmental Microbiology: 4367-4374.

4. Duffy, G., Riordan D. and Sheridan, J. (1999) Development of a critical control step for E.coli O157:H7 in pepperoni. Final Report Project ARMIS No. 4368.

5. Erdogrul, O. and Erbilir, F. (2006) Isolation and Characterization of Lactobacillus bulgaricus and Lactobacillus casei from Various Foods. Turk J Biol. 30; 39-44.

6. Guessas, B. and Kihal, M. (2004) Characterization of lactic acid bacteria isolated from Algerian arid zone raw goats’ milk. African Journal of Biotechnology: 339-342.

7. Parente, E. and RicciardiI, A. (1999) Production, recovery and purification of bacteriocins from lactic acid bacteria. Mini-Review Applied Microbial Biotechnology. 52: 628-638.

8. Rodríguez, A. (1988) Manual de Elaboración de Productos Lácteos Fermentados. Universidad Nacional de Colombia y Junta del Acuerdo de Cartagena.

9. Salminen, S., von Wight, A. and Ouwehand, A. (2004) Lactic acid bacteria: microbiological and functional aspects. Marcel Dekker. 3rd Edition, New York.

10. Savadogo, A., Ouattara, C., Savadogo, P. Ouattara, A., Barro, N. and Traore, A. (2004) Microorganisms Involved in Fulani Traditional Fermented Milk in Burkina Faso. Pakistan Journal of Nutrition: 134-139.

11. Sharpe M. (1979) Identification of the lactic acid bacteria. In: F/A/ Skinner and DW Lovelock (editors), Identification Methods for Microbiologists. Academic Press, London, pp. 233-259.

12. Steinkraus, K.H. (2002) Fermentations in World Food Processing. Comprehensive Reviews in Food Science and Food Safety. Vol. 1: 23-32.

13. Sulieman, A., Ilayan, A., Faki, A. (2006) Chemical and microbiological quality of Garris, Sudanese fermented camel’s milk product. International Journal of Food Science & Technology, Volume 41, Number 3, 321-328.

14. Togo, C. Feresu, S. and Mutukumira, A. (2002) Identification of Lactic Acid Bacteria isolated from Opaque beer (Chibuku) for potential use as a starter culture. The Journal of Food Technology in Africa: 93-97.

15. van Beek, S. and Priest, F. (2002) Evolution of the Lactic Acid Bacterial Community during Malt Whisky Fermentation: a Polyphasic Study. Applied and Environmental Microbiology: 297-305.

16. Wacher, C., Cañas, A., Barzana, E. Lappe, P., Ulloa, M., and Owens, J.D. (2000) Microbiology of Indian and Mestizo pozol fermentations. Food Microbiology. 17: 251-256.