2007, Number 3
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Cir Gen 2007; 29 (3)
Partial splenic autotransplantation, viability and immune response. Animal model
Golffier RCE, Quijano OF
Language: Spanish
References: 29
Page: 207-212
PDF size: 138.81 Kb.
ABSTRACT
Objective: To assess the efficacy, viability, and function of spleen autotransplantation in the vascularized region (inguinal) of the rat, corroborating its viability through hepato-splenic gammagraphy.
Design: Experimental in animals, prospective and longitudinal.
Setting: Experimental surgery unit, ABC Medical Center.
Statistical analysis: Percentages as summary measures for qualitative variables.
Material and methods: Wistar female rats, 4-month-old, 250-300 g weight. A total of 22 rats were subjected to 36 transplants. A control and an experimental group were formed, we performed laparotomies, splenectomies and bilateral inguinal implants. At the end of 14 months, a hepato-splenic gammagram was taken.
Results: The protocol was completed in all 22 animals, without these showing any infection or behavioral changes. All 36 implants revealed a good up-take of the radio-pharmaceutical agent as assessed with hepato-splenic gammagraphy, allowing for the identification of 100% of the implants.
Conclusions: The viability and function of the autologous splenic implant in rats were demonstrated as an option to preserve the lymphoid tissue in the organism.
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