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ISSN 0185-6014 (Print)
Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2007, Number 2

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Rev Mex Patol Clin Med Lab 2007; 54 (2)

Culturing the sediment of 50 mL does not improve significantly the diagnosis of dialysis-associated peritonitis

Rodríguez-Frausto M, Medina H, Macías AE

Language: Spanish
References: 19
Page: 72-77
PDF size: 124.12 Kb.


ABSTRACT

Culturing the centrifuged pellet of 50 mL does not improve significantly the diagnosis of dialysis-associated peritonitis. Background: To improve the diagnosis of dialysis-associated peritonitis, culturing the centrifuged pellet of 50 mL has been proposed. Methods: Study of 144 specimens from 144 patients with peritonitis associated to dialysis, without antibiotic treatment. The specimens were processed by three methods: 1) direct plating; 2) culturing the pellet of 50 mL; and 3) inoculating 10 mL in blood-culture bottle (gold standard). The result of the cultures and the identification of the germs were blinded. Results: Culture is blood-culture bottles rendered 92 isolates (63.9%). Of the centrifuged specimens, 73 isolations were obtained (50.7%), with sensitivity of 0.76, and specificity of 0.95. The technique of direct culture in agar plate found 54 isolations (37.5%) with sensitivity of 0.57, and specificity of 0.95. Conclusions: When compared against the gold standard, culturing the sediment of 50 mL is cumbersome and it does not significantly improve the efficiency of culturing the dialysis fluid.


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