Rosas-Velasco C, Pérez-Martínez M, Castillo-Juárez H, Flores-Pérez FI

Language: English/Spanish
References: 27
Page: 207-215
PDF size: 376.03 Kb.

ABSTRACT

The objective of this study was to evaluate the effect of medroxyprogesterone acetate (MPA) on the number and tissular distribution of CD14+ macrophages and BB-2 lymphocytes. A total of 15 white New Zealand rabbits were ovariectomized and, 3 weeks after surgery, 5 groups were formed and all animals, except the control group, received 5 mg I M of medroxyprogesterone acetate (MPA). Animals were euthanized at days 1 (early phase), 3 (medium phase) 6 and 7 (delay phase) post-treatment. The control group was euthanized at day 1 post-treatment. Fragments of uterine tissue were obtained and processed for inclusion in paraffi n. Through immunocytochemistry CD14+ macrophages and BB-2 lymphocytes were detected. The height of the secreting epithelium and the thickness of lamina propria were measured. Results indicate that MPA induces a decrease in the number of BB-2 positive cells at days 1, 3, 6 and 7 post-treatment. A biphasic effect was observed at days 1, 3, and 6 in which, the number of CD 14+ macrophages increased and fi nally at 7 days post-treatment the number of CD14 positive cells was similar to the control group. The height of glandular epithelium, at days 3, 6 and 7 post-treatment, showed an increment in contrast with the control group. Moreover, the lamina propria presented differences on its thickness only in days 1 and 7 vs control group. The results obtained suggest that MPA negatively affects the specifi c local immune response in the uterus of ovariectomized rabbits.

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