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2007, Number 1

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Bioquimia 2007; 32 (1)

Chlorophyllin as modulator and DNA damage protector: in vivo mouse system experience

García-Rodríguez MC, Altamirano-Lozano MA

Language: Spanish
References: 73
Page: 15-24
PDF size: 138.70 Kb.


ABSTRACT

Exposure of human populations to different environmental xenobiotic agents has generated considerable attention in the use of dietary supplements particularly plant products because exists an inverse correlation between the consumption of vegetables and incidence of cancer. These search and study of substances with protective properties or damage modulators to DNA emerge as a complementary option to genotoxicity studies. The analysis of the protection mechanisms generates alternatives to counteract the effects of agents causing the genotoxicity damage. Chlorophyllin (CHL) is a sodium-copper chlorophyll salt with a flat porphyrin ring and a metal ion atom in the center, with sodium or potassium atoms replacing the phytil ester groups. Several authors have demonstrated the antimutagenic and anticarcinogenic effects of CHL in live organisms including humans. Results obtained in our laboratory show that when CHL was administered i.p. to normal mice, and pregnant female mice, it had a protective effects against genotoxic damage induced by metals such CrO3 in all animals, including the fetuses, with out toxicity in the treated animal. The same results were observed using gamma radiation. On the other hand when pregnant females were exposed to high doses of CHL, its induce total litter loss, however when CrO3 was injected to pregnant mice resulted in significant increase in the external and skeletal malformations. CHL treatment before chromium injection resulted in a decreased in the external alterations. According the previous studies, the probable antimutagenic mechanisms of CHL are scavenging of free radicals and active oxygen species, suppression or interference with metabolic activation by specific cytochrome (P-450) and others metabolizing enzymes, and formation of complexes with promutagens or reactive moieties.


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