medigraphic.com
Revista Latinoamericana de Microbiología

2004, Number 1-2

Next >>

Microbiología 2004; 46 (1-2)

Resistance of Enterococcus strains isolated from pigs to gastrointestinal tract and antagonistic effect against Escherichia coli K88

García-Galaz A, Pérez-Morales R, Díaz-Cinco M, Acedo-Félix E

Language: English
References: 43
Page: 5-11
PDF size: 81.04 Kb.


ABSTRACT

The intestinal flora plays an important role in health and wellbeing of different organisms. Indigenous microflora can be innocuous or pathogenic. Consumption of food supplemented with beneficial microorganisms as probiotics provides a good health state and this can be maintained and recovered. Currently, probiotic strains of Bifidobacterium and Lactobacillus are widely used in humans as well as animals. Swine industry would benefit with the application of probiotics, mainly to overcome diarrheal diseases produced by different causes, as a pathogenic E. coli K88. The aim of this work was to isolate strains of Enterococcus from gastrointestinal tract of pigs to use them as probiotic. Two strains of E. faecalis, 2 of E. mundii and 7 of E. faecium were isolated with characteristics of resistance to acid pH, tolerance to biliary salts and a high antagonistic activity (>80%) against E. coli K88. Based on their characteristics and species affinity, we believe that these strains could be administered to pigs as a probiotic.


REFERENCES

  1. Audisio, M.C., G. Oliver, and M.C. Apella. 1999. Antagonistic effect of Enterococcus faecium J96 against human and poultry pathogenic Salmonella spp. J Food Prot. 62(7):751-755.

  2. Audisio, M.C., G. Oliver, and M.C. Apella. 2000. Protective effect of Enterococcus faecium J96, a potential probiotic strain, on chicks infected with Salmonella Pullorum. J Food Prot. 63(10):1333-1337.

  3. Audisio, M.C., G. Oliver, and M.C. Apella. 2001. Effect of different complex carbon sources on growth and bacteriocin synthesis of Enterococcus faecium. Int J Food Microbiol. 63:235-241.

  4. Beerens, H. 1991. Detection of Bifidobacteria by using propionic acid as a selective agent. Appl Environ Microbiol 57(8):2418-2419.

  5. Collins, J.K., G. Thornton, and G.O. Sullivan. 1998. Selection of probiotic strains for human applications. Int Dairy J. 8:487-490.

  6. Collins, M.D., and G.R. Gibson. 1999. Probiotics, prebiotics, and symbiotics: approaches for modulating the microbial ecology of the gut. Am J Clin Nutr. 69(suppl):1052-1057.

  7. Corona, V. 2001. Aislamiento y caracterización de Bifidobacterium sp a partir del contenido gástrico del cerdo. Tesis. Universidad de Sonora.

  8. CSPI news. 1999 FDA should ban the use of certain antibiotics to fatten farm animals.http://www.cspinet.org/new/antibiotics.htm

  9. De Martinis, E., V. Alves, and B. Franco. 2002. Fundamentals and perspectives for the use of bacteriocins produced by lactic acid bacteria in meat products. Food Reviews Int. 18(2):191-208.

  10. Dunne, C., L. Murphy, S. Flinn, L. O´Mahony, S. O´Halloran, M. Feeney, D. Morrisey. 1999. Probiotic: from myth to reality. Demonstration of functionally in animal models of disease and in human clinical trials. Anton Van Leeuwenhoek. 76:279-292.

  11. Falk, P.G., L.V. Hooper, T. Midtvedt, and J.I. Gordon. 1998. Creating and maintaining the gastrointestinal ecosystem: What we know and need to know from gnotobiology. Microbiology and Molecular Biology Reviews. 62(4):1157-1170.

  12. Forbes, B.A., D.F. Sahm, and A.S. Weissfeld. 1998. Bailey & Scott´s diagnostic microbiology. 10th ed. Mosby. USA.

  13. Gardiner, G., C. Stanton, P.B. Lynch, J.K. Collins, G. Fitzgerald, and R.P. Ross. 1999. Evaluation of cheddar cheese as a food carrier for delivery of a probiotic strain to the gastrointestinal tract. Journal of Dairy Science. 82:1379-1387.

  14. Gibson, G., D. Heber, S. Meydani, E. Postaire, and M.E. Sanders. 2000. Functional Dairy Products. John Libbey Eurotext. France.

  15. Gomes, A. and F. Malcata. 1999. Bifidobacterium spp and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics. Trends in Food Science and Technology. 10:139-157.

  16. Gómez. A., G. Kociubinski, P. Pérez, and G. De Antoni. 1998. Isolation and characterization of Bifidobacterium strains for probiotic formulation. J Food Prot. 61(7): 865-873.

  17. González, S.N., M.C. Apella, N.C. Romero, M.E. Nader de Macias, and G. Oliver. 1993. Inhibition of enteropathogens by lactobacilli strains used in fermented milk. J Food Prot. 56(9):773-776.

  18. Hernández, F. 2003. Algunos aspectos de investigación básica y aplicada sobre probióticos. Memoria Electrónica del 3er Simposio Mexicano de Probióticos. México, D.F.

  19. Hintze, J.L. 1997. NCSS 97 user’s guide—II. [Computer software manual.] Kaysville, UT: Number Cruncher Statistical Systems.

  20. Holt, J., N.R. Krieg, P.H. Sneath, J.T. Staley, and S.T. Williams, 1994. Bergey´s manual of determinative bacteriology. 9th ed. William & Wilkins. USA.

  21. Izeta M., J. 1992. Agentes etiológicos más comunes en el síndrome diarréico de los lechones. Síntesis Porcina. 11(2):36-40.

  22. Kociubinski, G., P.F. Pérez, M.C. Añon, and G.L. De Antoni. 1996. A method of screening for highly inhibitory lactic acid bacteria. J Food Prot. 59(7):739-745.

  23. Kociubinski, G., P. Pérez, and G. De Antoni. 1999. Screening of bile resistance and bile precipitation in lactic acid bacteria and bifidobacteria. J Food Prot. 62:905-912.

  24. Koneman, E.W., S.D. Allen, W.M. Janda, P.C. Schreckenberger, and W.C. Winn. 1994. Introduction to diagnostic microbiology. Lippincott Company. USA

  25. Laukova, A., S. Czikkova, Z. Vaslikova, P. Juris, and M. Marekova. 1998. Ocurrence of bacteriocin production among environmental enterococci. Letters in Applied Microbiology, 27:178-182.

  26. MacFaddin, J. F. 2000. Biochemical Tests for Identification of Bacteria of Medical Importance. Lippincott Williams & Wilkins. 3rd. edition. USA.

  27. McKellar, Q.A. 1998. Antimicrobial resistance: a veterinary perspective. British Medical Journal. 317:610-611.

  28. Naidu, A.S., W.R. Bidlack and R.A. Clemens. 1999. Probiotic spectra of lactic acid bacteria (LAB). Critical Rev. in Food Sci. and Nutrition. 38:13-126.

  29. Netherwood, T., H.J. Gilbert, D.S. Parker, and A.G. O´donnell. 1999. Probiotics shown to change bacterial community structure in the avian gastrointestinal tract. Appl Environ Microbiol. 65(11): 5134-5138.

  30. Nielsen, O.H., S. Jorgensen, K. Pedersen, and T. Justesen. 1994. Microbiological evaluation of jejunal aspirates and faecal samples after oral administration of bifidobacteria and lactic acid bacteria. Journal of Applied Bacteriology. 76:469-474.

  31. NOM-092-SSA1-1994. Norma Oficial Mexicana. Bienes y Servicios. Método para la cuenta de bacterias aerobias en placa.

  32. Pacheco, M. 2002. Aislamiento e identificación de Lactobacillus sp del intestino y contenido intestinal del cerdo, por medio de técnicas bacteriológicas clásicas y caracterización parcial de sus propiedades probióticas. Tesis. Universidad de Sonora.

  33. Prasad, J., H. Gill, J. Smart, and P.K. Gopal. 1998. Selection and characterization of Lactobacillus and Bifidobacterium strains for use as probiotics. Int Dairy J. 8:993-1002.

  34. Roberfroid, M.B. 2000. Prebiotics and probiotics: are they functional foods?. Am J Clin Nutr. 71(suppl):1682-1687.

  35. Rolfe, R.D. 2000. The role of probiotic cultures in the control of gastrointestinal health. Journal of Nutrition. 130:396-402.

  36. Salminen, S., A.C. Ouwehand, and E. Isolauri. 1998. Clinical applications of probiotic bacteria. Int Dairy J. 8:563-572.

  37. Salminen, S., A. von Wright, L. Morelli, P. Marteau, D. Brassart, W.M. de Vos, R. Fondén, M. Saxelin, K. Collins, G. Mogensen, S. Birkeland, T. Mattila. 1998b. Demonstration of safety of probiotics – a review. Int J Food Microbiol. 44:93-106.

  38. Shah, H., J. Lee, J. Pestka, and Z. Unstund. 1999. Viability of bifidobacteria in commercial dairy products during refrigerated storage. J Food Prot. 63(3):327-331.

  39. Shortt, C. 1999. The probiotic century: historical and current perspectives. Trends in Food Science & Technology. 10:411-417.

  40. Thornton, G., M. O´Sullivan , G. O´Sullivan, and J. Collins. 1993. Probiotic properties of Lactobacilli and Bifidobacteria. In Report of the Second Annual Meeting of Contractans. National Dairy Products Research Center.

  41. Trieu-Cuot, P. y C. Poyart. 1999. Visita con guía al centro del arsenal bacteriano. Mundo Científico. 197:46-50.

  42. Vandenbergh, P.A. 1993. Lactic acid bacteria, their metabolic product and interference with microbial growth. FEMS Microbiology Reviews. 12:221-238.

  43. Yen, J. 2001. Digestive System. In Biology of the Domestic Pig. Edited by W. Pond and H. Mersmann. Cornell University. Press USA.




2020     |     www.medigraphic.com