Low cost latex agglutination test (LAT) for the procurement of plasma containing antibodies against hepatitis B surface antigen

Sergio A Oviedo; Jorge A Zarzur

2004, Number 3

<< Back Next >>

Rev Mex Patol Clin Med Lab 2004; 51 (3)

Low cost latex agglutination test (LAT) for the procurement of plasma containing antibodies against hepatitis B surface antigen

Oviedo SA, Zarzur JA

Full text

How to cite this article

Language: Spanish
References: 24
Page: 171-177
PDF size: 69.36 Kb.

ABSTRACT

Objetivos: En este trabajo, se describe un test de aglutinación látex específico y sencillo (LAT) destinado a la búsqueda y detección de anticuerpos contra el virus de la hepatitis B (VHB) en muestras de plasma humano destinado a la producción de la inmunoglobulina específica. Métodos y resultados: El test reveló tener una buena correlación con enzimoinmunoensayo (EIA), (r:0.91) y el coeficiente de variación interensayo se encontró entre 8-15% demostrando una reproducibilidad adecuada para nuestros propósitos. La sensibilidad fue ajustada a niveles de anticuerpo iguales a 0.1 UI/mL y la especificidad fue determinada por ensayos competitivos. Conclusión: El procedimiento descrito resulta ser de bajo costo y de fácil aplicación, por lo que puede ser aplicado en el estudio y en la búsqueda poblacional de donantes de sangre para la obtención de plasma hiperinmune de la región, destinado a la producción de una inmunoglobulina contra el virus de la hepatitis B.

REFERENCES

Barbara JAJ, Howell DR, Tedder RS. Boosting of anti-HBs donors to increase supply of HBIg. Brief Report. British Blood Transfusion Society, Cambridge, England, 1983: 15-20.
Barbara JAJ, Howell DR. A Comparison of Different Methods of Screening Blood Donations for HBsAg. Vox Sanguinis 1977; 32: 4-9.
Hadler SC, Fay OH, Pinheiro F, Maynard JE. Las hepatitis en las Américas: Informe del Grupo Colaborador de la OPS; Bol Ofic San Panam 1987: 103.
Hophins R, Ross S, Barr A, McClelland DBL. Selection of plasma for Hepatitis B immunoglobulin using a low cost radioimmunoassay. Vox Sanguinis 1981; 40: 6-11.
Leen CLS, Uson R, Yap PL, McClelland DBL. Immune hepatitis B plasma procurement. Vox Sanguinis 1985; 49: 323-326.
Mahoney FJ et al. Effect of a hepatitis B vaccination Program on the Prevalence hepatitis B virus infection. J Infect Dis 1993; 167: 203-207.
Racela LS, Tegtmeier GE, Hodges GR, Reed JS. Comparison of two methods to determine Hepatitis B surface antibody response to hepatitis B vaccine among health-care workers. Am J Clin Pathol 1986; 86: 4-12.
Seef LB, Hoofnagle JH. Immunoprophylaxis of viral hepatitis. Gastroenterology 1979; 77: 161-182.
World Health Organization. International Conference on the Control of Hepatitis B in the developing World. Cameroon 1991: 7-9.
World Health Organization. Consensus statement on screening of blood donnations for infectious agents transmissible throug blood transfusion. WHOLBS91.1. 1991.
Booth JR, Nuttall PA. A rapid automated latex screen for tetanus toxoid antibodies. Vox Sanguinis 1979; 34: 239-240.
Elavia AJ, Rhomas A. Performance evaluation of a particle agglutination test for antibody to human immunodeficiency virus 1, comparison with enzyme immunoassay. Vox Sanguinis 1995; 69: 23-26.
Shibata Y, Baba M, Kuniyuki M. Studies on the retention of passively transferred antibodies in man. (I). Quantitative measurement of the antibody activities to hbs radioimmunoassay and its application to studies on the blood level of intravenously administered 7S or 5S antibody. Vox Sanguinis 1982; 43: 205-211.
Shibata Y, Baba M, Kuniyuki M. Studies on the retention of passively transferred antibodies in man. (II). Antibody activity in the blood after intravenous or intramuscular administration of anti-HBs human immunoglobulin. Vox Sanguinis 1983; 45: 77-82.
Cantarero LA, Butier JE, Osborne JW. The absorptive characteristics of proteins for polystyrene and their significance in the solid phase immunoassays. Analyt Biochem 1980; 105: 375-382.
Guerin JL, Holland PV, Purcell RH. Au-Ag large scale purification from human serum and biochemicals studies of its proteins. J Virol 1970; 7: 569-576.
De Rizzo et al. Concentration and purification of hepatitis B antigen with polyethylene glycon and polyelectrolyte 60, a crossliked copolymer of isobutylene maleic anhydrine. Infect Immunol 1972; 6: 335-346.
Oviedo SA, Zarzur JA. Diseño de una política de obtención de plasma con altos niveles de AcHBs, destinado a la producción industrial de una IgHb: “Screening Poblacional en un Banco de Sangre Universitario”. Revista SAFYBI 1998; 37 (97): 9-14.
Passing H, Bablock WJ. A new biometrical procedure for testing the equality of measurements from two different analytical methods. J Clin Chem. Clin Biochem 1983; 21: 709-720.
Zalazar F, Chiabrando GA, Aldao M, Vides MA. Parameters affecting the adsorption of ligands to polivinyl-chloride plates in enzyme immunoassays. J Immunol Methods 1992; 152: 1-7.
Fritz RB, Rivers SL. Hepatitis associated antigen; detection by antibody sensitized latex particles. J Immunol 1972; 106(1): 108-115.
De Witt JA, Van De Ven TGM. The effect of neutral polymers and electrolyte on the stability of aqueous polystyrene latex. Advances in colloid and interface. Science 1992; 42: 41-64.
European Pharmacopoeia. Human Hepatitis B Immunoglobulin 1997; 722: 1131.
Oviedo SA, Zarzur JA. Rapid Latex agglutination test for antibody to hepatitis B surface antigen: Performance study and comparison with enzime immunoassay. Rev Mex Patol Clin 2004; 51(2): 93-96.