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2022, Number 1

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Biotecnol Apl 2022; 39 (1)

Naphthalene exposure decreases reduced glutathione in male Wistar rats

Olaoye I, Awotula A, Oso B, Agboola O, Akhigbe G, Olaoye T

Language: English
References: 48
Page: 1201-1210
PDF size: 2507.48 Kb.


ABSTRACT

The study assessed the probable effects of naphthalene exposure through in vivo and in silico approaches. The in vivo assessment was carried on the glutathione (GSH) level in twenty-four Wistar Rats in two main groups based on different exposure times (2 and 4 h). These two groups were sub-grouped into three groups of four rats per group for 14 days. The in silico study was done on naphthalene and its metabolite towards glutamate-cysteine ligase (GCL) (the regulatory enzyme in GSH synthetic pathway) and glutathione reductase (GR). The result revealed that for 2 h of naphthalene exposure, the lower dose (0.75 g/m3) showed the highest reduced GSH level (93.50 ± 4.33 μmol/L) while the higher dose (1.50 g/m3) showed the least reduced GSH level (59.25 ± 7.57 μmol/L). In almost similar pattern, the 4 h exposure of naphthalene at both doses depicted a significant reduction compared to the control. The molecular docking study substantiated with the dynamics study revealed that naphthalene and all its metabolites had better binding score than glutathione (4.63 ± 0.15 kcal/mol) towards GCL with a common interacting residues (Leu-62, Val-63, Arg-64 and Phe-96). However, 4-hydroxychalcone had the best binding score (7.43 ± 0.58 kcal/mol) towards GR compared naphthalene and its metabolites. This study suggested that all naphthalene metabolites could possess more inhibitory potency than naphthalene towards GCL (except naphthalene epoxide) and GR.


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