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2022, Número 1

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Biotecnol Apl 2022; 39 (1)


Disminución de glutatión reducido en ratas Wistar machos tras la exposición a naftaleno

Olaoye I, Awotula A, Oso B, Agboola O, Akhigbe G, Olaoye T

Idioma: Ingles.
Referencias bibliográficas: 48
Paginas: 1201-1210
Archivo PDF: 2507.48 Kb.


RESUMEN

En el estudio se evaluó los posibles efectos de la exposición al naftaleno (GSH) mediante abordajes in vivo e in silico. En el estudio in vivo se evaluó los niveles de glutatión en 24 ratas Wistar, distribuidas en dos grupos principales a 2 y 4 h de exposición. Los grupos se subdividieron en tres subgrupos de 4 ratas cada uno, durante 14 días. En el estudio in silico se analizaron el naftaleno y sus metabolitos con respecto a la ligasa de glutamato-cisteína (GCL; enzima reguladora de la ruta sintética de GSH) y la glutatión reductasa (GR). Tras 2 h de exposición a naftaleno, la dosis menor (0.75 g/m3) mostró los mayores niveles de GSH reducido (93.50 ± 4.33 μmol/L), mientras que la dosis mayor (1.50 g/m3) los menores niveles (59.25 ± 7.57 μmol/L). Con un patrón casi similar, la exposición a ambas dosis de naftaleno durante 4 h provocó una disminución significativa en comparación con el control. El estudio de acoplamiento molecular complementado con el análisis de dinámica molecular reveló que el naftaleno y sus metabolitos tuvieron un mejor coeficiente de unión hacia la GSL que el GSH (4.63 ± 0.15 kcal/mol), al interactuar con aminoácidos similares (Leu-62, Val-63, Arg-64 and Phe-96). Sin embargo, la 4-hidroxichalcona mostró el mejor coeficiente de unión con la GR. El estudio sugirió que los metabolitos del naftaleno pueden mostrar una potencia inhibidora mayor que el naftaleno hacia la GCL (con excepción del epóxido de naftaleno) y la GR.


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