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Anales de Otorrinolaringología Mexicana

2022, Number 1

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Otorrinolaringología 2022; 67 (1)

Characterization of the biological fingerprint of nasal mucosa vs nasal polyps by FTIR spectroscopy

Sánchez-Santa Ana JR, Vázquez-Zapién GJ, Romo-Magdaleno JA, García-Perez E, Mata-Miranda MM

Language: Spanish
References: 22
Page: 32-39
PDF size: 274.74 Kb.


ABSTRACT

Objective: To compare the biological footprint of nasal mucosa vs nasal polyps using FTIR spectroscopy to determine biomolecular changes.
Materials and Methods: A prospective, observational, descriptive, cross-sectional, case-control study was done taking samples of healthy mucosa and of sinonasal polyps from patients with chronic polypoid rhinosinusitis. They were analyzed by FTIR spectroscopy in attenuated total reflectance mode and their biochemical mapping function. Likewise, histopathological analysis was performed using hematoxylineosin staining.
Results: There were included 6 samples of healthy mucosa and 14 of sinonasal polyps. The biological footprint of the healthy nasal mucosa, as well as of inflammatory sinonasal polyps, was determined by FTIR spectroscopy, finding significant differences in the regions of proteins, carbohydrates and nucleic acids (collagen and glycosaminoglycans). The histological findings of the polyps were mainly squamous metaplasia, mucosal edema, and inflammatory infiltrate.
Conclusions: FTIR spectroscopy makes it possible to differentiate between healthy nasal mucosa and inflammatory nasal mucosa, thus proposing FTIR spectroscopy as an alternative for the preoperative study of patients with chronic polypoid rhinosinusitis.


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