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Anales de Otorrinolaringología Mexicana

2022, Número 1

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Otorrinolaringología 2022; 67 (1)


Caracterización de la huella biológica de mucosa nasal vs pólipos nasales mediante espectroscopia FTIR

Sánchez-Santa Ana JR, Vázquez-Zapién GJ, Romo-Magdaleno JA, García-Perez E, Mata-Miranda MM

Idioma: Español
Referencias bibliográficas: 22
Paginas: 32-39
Archivo PDF: 274.74 Kb.


RESUMEN

Objetivo: Comparar la huella biológica de mucosa nasal vs pólipos nasales usando espectroscopia FTIR para determinar los cambios biomoleculares.
Materiales y Métodos: Estudio prospectivo, observacional, descriptivo, transversal de casos y controles en el que se tomaron muestras de mucosa sana y de pólipos nasosinusales de pacientes con rinosinusitis crónica polipoidea. Se analizaron mediante espectroscopia FTIR en modo de reflectancia total atenuada y su función de mapeo bioquímico. Asimismo, se realizó análisis histopatológico mediante tinción de hematoxilina-eosina.
Resultados: Se incluyeron 6 muestras de mucosa sana y 14 de pólipos nasosinusales. Se determinó mediante espectroscopia FTIR la huella biológica de la mucosa nasal sana y de pólipos inflamatorios nasosinusales, encontrando diferencias significativas en las regiones de proteínas, carbohidratos y ácidos nucleicos (colágeno y glucosaminoglicanos). Los hallazgos histológicos de los pólipos fueron principalmente metaplasia escamosa, edematización de la mucosa e infiltrado inflamatorio.
Conclusiones: La espectroscopia FTIR permite diferenciar entre mucosa nasal sana y mucosa nasal inflamatoria, con lo que se propone a la espectroscopia FTIR como alternativa de estudio preoperatorio del paciente con rinosinusitis crónica polipoidea.


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