Villalobos-Arámbula AR, Barragán RDM

Language: Spanish
References: 24
Page: 167-177
PDF size: 403.71 Kb.

ABSTRACT

Documentation of biodiversity is increasingly dependent of DNA sequences, a method known as DNA barcoding. The results of the workflow stages of the semi-portable of the Barcode BioMol UDEG (BBG) project are presented, which consist of: DNA extraction, amplification by polymerase chain reaction, purification of PCR products, DNA sequencing, and comparison with a database. Furthermore, they participated in the formulation of questions, experimental design, data collections, and analysis to prepare a scientific report. 62 students to get involved, who in addition to their training in molecular biology techniques, they contributed to 21 to ITS sequences from fungi, two COI from ants and three from a plant chloroplast DNA (rbcL, matK, and trnH-psbA), which allowed the taxonomic determination of 24 specimens (11 at the species level, two close to the species, 9 to the genus and one specimen to the family) and evidenced the achievement of scientific skills from the students.

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