2016, Number 33
<< Back Next >>
Salud Quintana Roo 2016; 9 (33)
Aeromonas hydrophila in seafood of animal origen from establishments of Puerto Angel, Oaxaca, Mexico
Franco-Monsreal J, Lara-Zaragoza EB, Villa-Ruano N, Mota-Magaña L, Hernández-Gómez JR, Serralta-Peraza LES
Language: Spanish
References: 49
Page: 4-10
PDF size: 298.10 Kb.
ABSTRACT
Introduction: Within the genus
Aeromonas the
hydrophila
species is one of three clinically important. The spectrum of
human infections caused by this species include acute gastroenteritis
(AG), wound infection (WI), urinary tract infection
(UTI), primary septicemia (PS) and secondary septicemia
(SS). The objective of the study was to identify whether
seafood -raw, marinated without heat, partially cooked with
heat and completely cooked with heat- that are sold in stores
of Puerto Angel, Oaxaca, Mexico, represent potential risk
factors for the
Aeromonas hydrophila species for development
of AG, WI, UTI, PS and SS.
Material and Methods: A descriptive observational crosssectional
study in which four hundred samples were studied.
[325 (81,25%) raw, 17 (4,25%) marinated without heat, 18
(4,50%) partially cooked with heat and 40 (10,00%) completely
cooked with heat]. For homogenization and enrichment of
the samples, as well as for the isolation and identification of
Aeromonas hydrophila species proceeded according to the
methodology described in the Bacteriological Analytical
Manual (Food and Drug Administration). As statistical significance
test Chi-square Mantel-Haenszel (x²m-h) was used.
Results: The prevalences obtained in raw, marinated without
heat, partially cooked with heat and completely cooked with
heat seafood were, respectively, 49,54% (161/325), 35,29%
(6/17), 27,78% (5/18) and 15,00% (6/40). In one hundred
seventy eight (44,50%) samples an equal number of strains
whose biochemical characteristics corresponded to the
Aeromonas
hydrophila species were isolated.
Conclusions: We conclude that raw, marinated without heat,
partially cooked with heat and completely cooked with heat
seafood represent potential risk factors for
Aeromonas hydrophila
species for the development of AG, WI, UTI, PS and SS
at Puerto Ángel, Oaxaca, México.
REFERENCES
García-Martos P, Benjumeda M, Delgado D. Otitis externa por Vibrio alginolyticus: descripción de cuatro casos 1993; Acta Otorrinolaringol Esp. 44(1): 55-57.
Garrity GM, Bell JA, Lilburn TG. Taxonomic Outline of the Prokaryotes. Bergey’s Manual of Systematic Bacteriology. Second Edition. Release 5.0; 2004. p. 112-3.
Giono CS. Agentes bacterianos de infecciones gastrointestinales, en: Giono CS, Escobar GA y Valdespino GJ, eds. Diagnóstico de Laboratorio de Infecciones Gastrointestinales. México D.F.: Secretaría de Salud. 1994. p.175-9.
Sistema Nacional de Vigilancia Epidemiológica. México D.F.: Secretaría de Salud. 2001: 18, Semana 52.
Janda JM. Recent advances in the study of the taxonomy, pathogenicity and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev 1991;4:397-410.
Janda JM, Abbott SL. Evolving concepts regarding the genus Aeromonas an expanding panorama of species, diseases presentation, and unanswered questions. J Clin Infec Dis 1998;17: 332-44.
Rebollo B, Escamilla AE. Aislamiento e identificación de Aeromonas spp. y Plesiomonas shigelloides como causa de diarrea en humanos. INNSZ. Memorias Congreso Nacional Mexicano. Veracruz. 1984.
Martínez SP. Caracterización de cepas de Aeromonas aisladas de pacientes con síntomas de origen infeccioso en el Distrito Federal. Tesis de Licenciatura. Universidad Autónoma de Chiapas. México. 1996.
Franco-Monsreal J, Flores-Abuxapqui JJ, Suárez-Hoil GJ, Puc-Franco MA, Heredia-Navarrete MR, Vivas-Rosel ML. Prevalencia de Aeromonas hydrophila en alimentos marinos de origen animal de restaurantes de la ciudad de Mérida, Yucatán, México. 1999; Higiene. 1(3): 144-54.
Pavia AT, Bryan JA, Maher KL, Hester TR Jr., Farmer JJ III. Vibrio carchariae infection after a shark bite. 1989; Ann Intern Med. 111(1): 85-6.
Franco-Monsreal J, Flores-Abuxapqui JJ, Salas-Escajeda RM, Concha-Valdez FG, Puc-Franco MA, Heredia-Navarrete MR, Vivas-Rosel ML y Piña-Quijano JA. Aeromonas hydrophila, Vibrio alginolyticus y Vibrio fluvialis biovariedad II en alimentos marinos de origen animal de restaurantes de la ciudad y puerto de Progreso de Castro, Yucatán, México. 2008; Ciencia en la Frontera. 6(Número Especial): 45-57.
Franco-Monsreal J, Zarza-García AL, Villa-Ruano N, Ramón-Canul LG, Pacheco-Hernández Y, Cardeña-Bozziere IM, Mota-Magaña L. Aeromonas hydrophila en alimentos marinos de origen animal de coctelerías, cooperativa, pescaderías, restaurantes y supermercados de Isla del Carmen, Campeche, México. 2011; RedNutrición. 2(6): 13-8.
Disponible en: https://www.google.com.mx/search?hl=es&site= imghp&tbm=isch&source=hp&biw=1920&bih=911&q=puerto+ %C3%A1ngel+oaxaca&oq=puerto+%C3%81ngel&gs_l=img.1.1. 0l2.4593.11302.0.15085.12.12.0.0.0.0.661.1451.2-1j5-2.3.0....0...1ac.1.64. img..9.3.1449.z3qyx9_nBKI
Gobat P, Jemmi T. Comparison of seven selective media for the isolation of mesophilic Aeromonas species in fish and meat. Int J Food Microbiol 1995;24:375-84.
Janda JM. Aeromonas and Plesiomonas, in: Sussman M, ed. Molecular Medical Microbiology. San Diego: Academic Press; 2001. p. 1237-70.
Altwegg M. Aeromonas and Plesiomonas, in: Murray PR, Baron EJ, Pfaller MA, Tenover FC, eds. Manual of Clinical Microbiology. Washington: ASM press; 1999. p. 507-16.
Martínez MJ, Simon-Pujol D, Congregado F. The presence of capsular polysaccharide in mesophilic Aeromonas hydrophila serotypes O:11 and O:34. FEMS Microbiol Lett 1995;128:69-73.
Kokka RP, Janda JM, Oshiro LS, Altwegg M, Shimada T, Sakazaki R et al. Biochemical and genetic characterization of autoagglutinating phenotypes of Aeromonas species associated with invasive and noninvasive disease. J Infect Dis 1991;163:890-4.
Dooley JSG, McCubbin WD, Kay CM, Trust TJ. Isolation and biochemical characterization of the S-layer protein from a pathogenic Aeromonas hydrophila strain. J Bacteriol 1988;170:2631-8.
Kokka RP, Velki AM, Clark RB, Bottone EJ, Janda J. Immune response to S layer-positive O:11 Aeromonas associated with intestinal and extraintestinal infections. Immunol Infect Dis 1992;2:111-4.
Dooley JSG, Lallier R, Shaw DH, Trust TJ. Electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of Aeromonas hydrophila. J Bacteriol 1985;164:263-9.
Ho Asy, Sohel I, Schoolnik GK. Cloning and characterization of fxp, the flexible pilin gene of Aeromonas hydrophila. Mol Microbiol 1992;6:2725-32.
Kirov SM, Sanderson K. Characterization of a type IV bundleforming pilus (SFP) from a gastroenteritis-associated strain of Aeromonas veronii bv sobria. Microb Pathog 1996;21:23-34.
Pepe CM, Eklund MW, Strom MS. Cloning of an Aeromonas hydrophila type IV pilus biogenesis gene cluster: complementation of pilus assembly functions and characterization of a type IV leader peptidase/N-methyltransferase required for extracellular protein secretion. Mol Microbio 1996;19: 8578-91.
Jeanteur D, Gletsu N, Pattus F, Buckley JT. Purification of Aeromonas hydrophila major outer-membrane proteins: N-terminal sequence analysis and channel-forming properties. Mol Microbiol 1992;6:3355-63.
Pemberton JM, Kidd SP, Schmidt R. Secreted enzymes of Aeromonas. FEMS Microbiol Lett 1997;152:1-10.
Buckley JT, Howard SP. The cytotoxic enterotoxin of Aeromonas hydrophila is aerolysin. Infect Immun 1999;67:466-7.
Bernheimer AW, Avigad LS. Partial characterization of aerolysin, a lytic exotoxin from Aeromonas hydrophila. Infect Immun 1974;9:1016-21.
Howard SP, Garland WJ, Green MJ, Buckley JT. Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila. J Bacteriol 1987;169:2869-71.
Buckley JT. The channel – forming toxin aerolysin. FEMS Microbiol Immunol 1992;5:13-7.
Chopra AK, Houston CW. Enterotoxins in Aeromonas-associated gastroenteritis. Microbes Infect 1999;1:1129-37.
Albert JM, Ansaruzzaman M, Talukder KA, Chopra AK, Inger Kuhn, Rahman M et al. Prevalence of enterotoxin genes in Aeromonas spp. isolated from children with diarrhea, healthy controls, and the environment. J Clin Microbiol 2000;38: 3785-90.
Chopra AK, Xu XJ, Ribardo D, González M, Jul K, Peterson JM et al. The citotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect Immun 2000;5:2808-18.
Rodríguez LA, Ellis AE, Nieto TP. Purification and characterization of an extracellular metalloprotease, serine protease and haemolysin of Aeromonas hydrophila strain B32: all are lethal for fish. Microb Pathog 1992;13:17-24.
Cascón A, Yugueros J, Temprano A, Sánchez M, Hernanz C, Luengo JM et al. A major secreted enastase is essential for pathogenicity of Aeromonas hydrophila. Infect Immun 2000;68:3233-41.
Merino S, Aguilar A, Nogueras MM, Regue M, Swift S, Tomás JM. Cloning, sequencing and role in virulence of two phospholipases (A1 and C) from mesophilic Aeromonas sp. serogroup 0:34. Infect Immun 1999;67:4008-13.
Anguita J, Rodriguez-Aparicio LB, Navarro G. Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate. Appl Environ Microbiol 1993;59:2411-7.
Brumlik MJ, Buckley JT. Identification of the catalytic triad of lipase/ acyltransferase from Aeromonas hydrophila. J Bacteriol 1996;178:2060-4.
Vipond R, Bricknell IR, Durant E, Bowden TJ, Ellis AE, Smith M et al. Defined deletion mutants demonstrate that the major secreted toxins are not essential for the virulence of Aeromonas salmonicida. Infect Immun 1998;66:1990-8.
Chang MC, Chang SY, Chen SL, Chuang SM. Cloning and expression in Escherichia coli of the gene encoding an extracellular deoxyribonuclease (DNase) from Aeromonas hydrophila. Gene 1992;122:175-180.
Barghouthi S, Young R, Olson MOJ, Arceneaux JE, Clem LW, Byers BR. Amonabactin, a novel tryptophan- or phenylalanine-containing phenolate siderophore in Aeromonas hydrophila. J Clin Microbiol 1989;171:1811-6.
Stintzi A, Barnes C, Xu J, Raymond KN. Microbial iron transport via a siderophore shuttle: a membrane ion transport paradigm. Proc Natl Acad Sci 2000;97:10691-6.
Pavan ME, Abbot SL, Zorzopulos J, Janda JM. Aeromonas salmonicida subs. pectinolytica subs. nov., a new pectina sepositive subspecies isolated from a heavily polluted river. Int J Syst Evol Microbiol 2000;3:1119-24.
Cochran, W.G. Some methods for strengthening the common x² tests. Biometrics 1954;10(4): 417-51.
Daniel, W.W. (2014). Bioestadística: base para el análisis de las ciencias de la salud. Cuarta edición. México, D.F.: Editorial Limusa. pp. 91-2, 143-4.
Peffers, A.S., Bailey, J., Barrow, G.I. & Gobbs, B.C. (1973). Vibrio parahaemolyticus gastroenteritis and international air travel. Lancet. 1(7795): 143-5.
Fujino, T.T. (1967). Report of the food hygiene sub-committee on Vibrio parahaemolyticus. In: Fujino, T. & Fukumi, H. (ed). Vibrio parahaemolyticus. Nayashoten: 673-725.
Pérez-Memije, E., Vélez-González, M.L. y Galván-Rodríguez, F. (1980). Búsqueda de Vibrio parahaemolyticus en heces de manejadores de alimentos en el puerto de Acapulco, Guerrero. Rev Lat-amer Microbiol. 22(1): 18.
Franco-Monsreal, J. y Flores-Abuxapqui, J.J. (1988). Prevalencia de Vibrio parahaemolyticus en productos marinos y en heces de manipuladores de alimentos. Rev Lat-amer Microbiol. 30: 223-7.