Ferraz LF, Schezaro-Ramos R, Rodrigues-Simioni L, Randazzo-Moura P, Rostelato-Ferreira S

Language: Portugués
References: 19
Page: 1-14
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ABSTRACT

Introduction: venoms from snakes of the Bothrops genus are capable of inducing neuromuscular blockade in preparations isolated from mammals and birds. The specific treatment is serum therapy, but it is not always effective against the toxicity induced by the venom. On the other hand, some plant compounds have been studied for antiophidic purposes, as is the case with Camellia sinensis (green tea).
Objective: evaluate the activity of the alcoholic extract from Camellia sinensis against the action of various bothropic venoms in bird neuromuscular preparations.
Methods: Chick biventer cervicis (BC) neuromuscular preparations were used which were incubated with venoms from various snakes of the Bothrops genus (B. jararaca, B. jararacussu, B. alternatus and B. neuwiedi, 50-100 μg/ml), Camellia sinensis (Cs) alcoholic extract, 100 μg/ml, or previously treated with Cs (30 min), followed by the addition of the various bothropic venoms.
Results: blockade of the contractile response was observed in 69.7 ± 4.3% (B. jararaca), 83.1 ± 10% (B. jararacussu), 88.1 ± 4.5% (B. alternatus) and 92.4 ± 5.6% (B. neuwiedi). When the preparations were previously incubated with Cs extract, results were 62%, 89.2%, 75.1% and 78.2%, respectively.
Conclusion: Camellia sinensis extract was found to be effective against neurotoxicity induced by bothropic venoms.

REFERENCES

1. Pinho FMO, Pereira ID. Ofidismo. Rev Ass Med Brasil 2001;47(1):24-9.

2. De Oliveira RC, Wen RC, Sifuentes FH. Epidemiologia dos Acidentes por Animais Peçonhentos. In: Cardoso JLC, França SFO, Wen FH, Málaque CMS, Haddad Junior V. (Eds). Animais peçonhentos no Brasil: biologia, clínica e terapêutica dos envenenamentos. São Paulo: Sarvier 2009. p.6-21

3. Moura da Silva AM, Cardoso DF, Tanizaki M. Differences in distribution of myotoxic proteins in venoms from differents Bothrops species. Toxicon 1990. 28:1293-301

4. Cavalcante WLG. Caracterização das atividades de PLA2s Lys49, isoladas de venenos de serpentes do gênero Bothrops, em preparação neuromuscular de camundongos e influência de agentes neutralizadores. 2009. pp. 64. Tese (Doutor em Farmacologia). Instituto de Biociências, Universidade Estadual Paulista Julio de Mesquita Filho - Unesp, Botucatu.

5. Yanagimoto K, Ochi H, Lee KG, Shibamoto T. Antioxidative activities of volatile extracts from green tea, oolong tea, and black tea. J Agric Food Chem 2003. 51:7396-401.

6. Cooper R, Morré J, Morré DM. Medicinal benefits of green tea: part II. Review of anticancer properties. The Journal of Alternative and Complementary Medicine 2005. 11:639-52.

7. De Jesus Reis Rosa L, Silva GA, Filho JA, Silva MG, Cogo JC, Groppo FC, et al. The inhibitory effect of Camellia sinensis extracts against the neuromuscular blockade of Crotalus durissus terrificus venom. J Venom Res 2010. 1:1-7.

8. Oshima-Franco Y, De Jesus Reis Rosa L, Silva GAA, Amaral Filho J, Silva MG, Lopes PS, Cogo JC, et al. Antibothropic Action of Camellia sinensis Extract Against the Neuromuscular Blockade by Bothrops jararacussu Snake Venom and Its Main Toxin, Bothropstoxin-I, Pharmacology, Dr. Luca Gallelli (Ed.), InTech. 2012. Available from: http://www.intechopen.com/books/pharmacology/antibothropic-action-of-camellia-sinensis-extract-against-theneuromuscular-blockade-of-bothrops-jar

9. Borges RC. Serpentes Peçonhentas Brasileiras: Manual de Identificação Prevenção e Procedimentos em Caso de Acidentes. Editora Atheneu, 1999.

10. Ginsborg BL, Warriner J. The isolated chick biventer cervicis nerve-muscle preparation. Br J Pharmacol Chemother 1960. 15:410-1.

11. Harvey AL, Barfaraz A, Thomsom E, Faiz A, Preston S, Harris JB. Screening of snake venoms for neurotoxic and myotoxic effects using simple in vitro preparations from rodents and chicks. Toxicon 1994. 32:257-65.

12. Zamuer SR, Crüz-Hofling MA, Corrado AP, Hyslop S, Rodrigues-Simioni L. Comparison of the neurotoxic and myotoxic effects of Brazilian Bothrops venoms and their neutralization by commercial antivenom. Toxicon 2004. 1(44):259-71.

13. Coërs C. Structure and organization of the myoneural junction. International review of cytology 1967. 22:239-67.

14. Cintra-Francischinelli M, Silva MG, Andreo-Filho N, Gerenetti M, Cintra ACO, Giglio JR, et al. Antibothropic action of Casearia sylvestris Sw. (Flacourtiaceae) extracts. Phytotherapy Research 2008. 22:784-90.

15. Mise YF, Casais-e-Silva L, Lira-da-Silva R. Ação protetora da Calendula officinalis (asteracea; compositae) sobre a atividade miotoxica do veneno Bothrops leucurus (serpentes; viperidae). Gazeta Médica da Bahia. (Supl.1)2009. 50-5.

16. Collaço RC, Rocha Junior DS, Silva MG, Cogo JC, Oshima-Franco Y, Randazzo-Moura P. Propriedade antiofídica do extrato metanólico de Mikania laevigata sobre as ações biológicas induzidas pelo veneno de Philodryas olfersii na junção neuromuscular. Revista de Estudos Universitários – REU 2010. 36(2).

17. Rostelato-Ferreira S, Rodrigues-Simioni L, Oshima-Franco Y. Heparin and bothropic commercial antivenom against the paralysing effect of Bothrops jararacussu snake venom. J Venom Anim Toxins incl Trop Dis 2010. 16(1):34-45.

18. Rostelato-Ferreira S, Leite GB, Cintra ACO, Cruz-Höfling MA, Rodrigues-Simioni L, Oshima-Franco Y. Heparin at low concentration acts as antivenom against Bothrops jararacussu venom and bothropstoxin-I neurotoxic and myotoxic actions. J Venom Research 2010. 1:54-60.

19. Randazzo-Moura P, Leite GB, Silva GH, Paffaro Junior VA, Cintra ACO, Cruz-Höfling MA, et al. A study of the myotoxicity of bothropstoxin-I using manganese in mouse phrenic nerve-diaphragm and extensor digitorum longus preparation. Braz J Morphol Sci 2006. 23(2):237-46.