2019, Number 4
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Rev Cubana Plant Med 2019; 24 (4)
Antioxidant activity of ethanolic extracts and essential oils from Gliricidia sepium (Jacq.) Kunth ex Walp. and propolis from Melipona beecheii Bennett
Fonte CL, Takahashi JA, Díaz SM, Santos DS, Durán ONP, Blandón OAM, Castro CI, Lugo MY, Altunaga PN
Language: Spanish
References: 40
Page: 1-15
PDF size: 508.89 Kb.
ABSTRACT
Introduction: Gliricidia sepium is a multi-purpose melliferous forage tree of interest to natural medicine. Production of propolis from exudates of various plants is a source of bioactive compounds.
Objective: Evaluate the antioxidant activity of ethanolic extracts and essential oils obtained from Gliricidia sepium and propolis from Melipona beecheii.
Methods: Ethanolic extracts were obtained from G. sepium flowers and bark during the flowering season and from propolis and flowers from the plant. Determination was made of total flavonoids and phenols, total antioxidant capacity and ferric reducing power of each of the extracts and oils. Data were analyzed by simple analysis of variance. Use was made of Infostat statistics package, free version. Comparison of means was conducted by Duncan's multiple range test with a significance level of p < 0.05.
Results: The propolis essential oil was the most active in the total antioxidant capacity test. The total amount of phenols detected in the samples ranged between 37.9 and 116.3 mg of gallic acid/g of dry material, whereas the content of flavonoids ranged between 7.51 μg/g and 13.18 μg/g. Ferric ion reducing percentage was highest for the propolis oil (95.2%) and 82.2% for the G. sepium flower oil.
Conclusions: In the four biological assays conducted propolis obtained the best results, both as ethanolic extract and as essential oil. It could therefore become a good candidate for use in Cuban industry and medicine.
REFERENCES
Kolawole OM, Joseph AK, George OA. Antibacterial and Phytochemical Activity of Gliricidia sepium against Poultry Pathogens. 2018.
Wilson MB, Brinkman D, Spivak M, Gardner G, Cohen JD. Regional variation in composition and antimicrobial activity of US propolis against Paenibacillus larvaeand, Ascosphaera apis. J. Invertebr. Pathol. 2015. 124:44-50.
Orantes Bermejo FJ. Los Propóleos en Andalucía. Grupo de Cooperación Columela-Asociación Provincial de Apicultores de Granada. Proyecto C/99/006, 2006.
Bankova VS, de Castro SL, Marcucci MC. Propolis: recent advances in chemistry and plant origin. Apidologie, 2000.31(1), 3-15.
Cumbao JLT, Alvarez PLJ, Belina-Aldemita MD, Micor JRL, Angelia MRN, Manila-Fajardo AC. Total phenolics, total flavonoids, antioxidant activity and antibacterial property of propolis produced by the stingless bee, Tetragonula biroi (Friese), from Laguna and Quezon, Philippines. Philippine Entomologist. 2016.
Ferreira JM, Fernandes‐Silva CC, Salatino A, Negri G, Message D. New propolis type from north‐east Brazil: chemical composition, antioxidant activity and botanical origin. Journal of the Science of Food and Agriculture, 2017. 97(11), 3552-8.
López A, et al. Antioxidantes, un paradigma en el tratamiento de enfermedades. Revista ANACEM, 2012. 6(1):49-51.
Kutaiba Ibrahim A, Mohamed Abdalkarim M. Flavonoids: Chemistry, Biochemistry and Antioxidant activity. Journal of Pharmacy Research, 2012, 5(8), 4013-20.
Carlsen, M. H., Halvorsen, B. L., Holte, K., Bohn, S. K.,Dragland, S., Sampson, L., & Blomhoff, R. The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide.2010, Nutrition Journal, 9, 3. https://doi.org/10.1186/1475-2891-9-3
Reyes A, Galicia M, Carrillo M. Antioxidantes: La magia de lo natural. Rev Tlatemoani. 2011;(8): 1-16.
Academia de Ciencias de Cuba. Nuevo Atlas Nacional de Cuba. Instituto Cubano de Geodesia y Cartografía. La Habana, Cuba. 1989, p. 41
Hernández JA, Pérez JJM, Bosch ID, Castro SN. Clasificación de los suelos de Cuba. Instituto Nacional de Ciencias Agrícolas (INCA) e Instituto de Suelos, Ministerio de Agricultura (MINAG). La Habana, Cuba, 2015. p 46-7.
Monsálvez MN, Zapata M, Vargas M, Berti M, Bittner V Hernández. Antifungal effects of n-hexane extract and essential oil of Drimys winteri bark against take-all disease. Ind. Crop. Prod.2010, 31(2): 239-44.
Martínez, A. “Aceites esenciales”. Universidad de Antioquia. Medellín. 2003,1-34.
Umamaheswari M, Chatterjee TK: In vitro antioxidant activities of the fractions of Coccinniagrandis L. leaf extract. Afr J Trad Compl Altern Med 2008, 5:61-73.
Arabshahi-Delouee S y Urroj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chemistry. 2007, 102: 1233-40.
Liu X, Zhu L, Tan J, Zhou X, Xiao L, Yang X et al. Glucosidase inhibitory activity and antioxidant activity of flavonoid compuond and triterpenoid compuond from Agrimonia pilosa Ledeb. Complementary and Alternative Medicine. 2014. 14:12.
Oyaizu M: Studies on product of browning reaction prepared from glucose amine. Japanese J. Nutr 1986, 44:307-15.
Paladino S. Actividad antioxidante de los compuestos fenólicos contenidos en las semillas de la vid (Vitis vinifera l.)-126 -Facultad de Ciencias Agrarias., Universidad Nacional de Cuyo., Mendoza -Argentina, TESIS, 2011. pp. 12-5.
Talla E, Tamfu AN, Gade IS, Yanda L, Mbafor JT, Laurent S et al. New Mono-Ether of Glycerol and Triterpenes with DPPH Radical Scavenging Activity from Cameroonian Propolis. Nat. Prod. Res. 2016, 6419 (November), 1-11.
Hernández M, Abraham M, Cerón A, Gutiérrez A, Gutiérrez D, Ávila F. Contenido de flavonoides, fenoles y actividad antioxidante de propóleos colectados en Guanajuato, México. 2017, 2,607-12.
Olczyk P, Komosinska K, Ramos P, Mencner L, Olczyk K, Pilawa B. Free Radical Scavenging Activity of Drops and Spray Containing Propolis-An EPR Examination Pawel. Molecules 2017, 22, 3-6.
Zhang C, Shen X, Chen J, Jiang X, Hu F. Identification of Free Radical Scavengers from Brazilian Green Propolis Using Off-Line HPLC-DPPH Assay and LC-MS. Food Technol. 2017, 14-9.
Kustiawan P, Lirdprapamongkol K, Palaga T, Puthong S, Phuwapraisirian P, Svasti J et al. Molecular Mechanism of Cardol, Isolated from Trigona incisa Stingless Bee Propolis, Induced Apoptosis in the SW620 Human Colorectal Cancer Cell Line. BMC Pharmacol.Toxicol.2017, 4-13.
Mise S, Köprücü K, Enis M, Silici S. Effects of Dietary Propolis on the Number and Size of Pleopadal Egg, Oxidative Stress and Antioxidant Status of Freshwater Cray Fish (Astacus Leptodactylus Eschscholtz). Elsevier 2017, No. June, 4-5.
Mujica V, Orrego R, Pérez J, Romero P, Ovalle P, Zúñiga J et al. The Role of Propolis in Oxidative Stress and Lipid Metabolism : A Randomized Controlled Trial. Evidence-based Complement. Altern. Med. 2017, 5-6.
Salmas R, Durdagi S, Fuat M, Duruyurek M, Abdullah H, Selamoglu Z. The Effects of Pollen, Propolis, and Caffeic Acid Phenethyl Ester on Tyrosine Hydroxylase Activity and Total RNA Levels in Hypertensive Rats Caused by Nitric Oxide Synthase Inhibition : Experimental, Docking and Molecular Dynamic Studies. J. Biomol. Struct. Dyn. 2017, 1102 (January), 0-1.
Pedreño Y. Los antioxidantes polifenólicos, un complemento alimenticio saludable, Revista Eubacteria. No. 28, Murcia -España, 2012. pp. 1-4.
Ignaz I, Volf I, Popa VI. A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food Chem. 2011, 126: 1821-35.
Escamilla C, Cuevas E, Guevara J. Flavonoides y sus acciones antioxidantes. Rev Fac Med UNAM. 2009; 52(2): 73-5.
Da Silva Frozza C, Garcia C, Gambato G, De Souza M, Salvador M, Moura S et al. A Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis. Food Chem.Toxicol.2013, 52, 13-42.
De Mendonça I, De Moraes Porto I, Do Nascimento TG, De Souza NS, Dos Santos Oliveira JM, Dos Santos Arruda RE et al. Brazilian Red Propolis: Phytochemical Screening, Antioxidant Activity and Effect against Cancer Cells. BMC Complement. Altern. Med. 2015, 1-12.
Betances E, Revilla I, Vivar A, González M. Flavonoid and Antioxidant Capacity of Propolis Prediction Using Near Infrared Spectroscopy. Sensors 2017, 17, 2-13.
Sun F, Hayami S, Haruna S, Ogiri Y, Tanaka K, Yamada Y. In vivo antioxidative activity of propolis evaluated by the interaction with vitamins C and E and the level of lipid hydroperoxidases in rats. J Agric Food Chem 2000; 48(5):1462-5.
Frión Y, Díaz A, Ruiz J, Rodríguez H, Maurício J. Brazilian Green Propolis Induced Apoptosis in Human Lung Cancer A549 Cells through Mitochondrial-Mediated Pathway. Journal of Pharmacy and Pharmacology, 2015. 67(10):1448-56.
Alizaldeh A, Afrouzan H, Dinparast N, Sawaya A, Azizian S, Hemmati H et al. Chemoprotection of MNNG-Initiated Gastric Cancer in Rats Using Iranian Propolis. 2015.
Elnakady Y, Rushdi A, Franke R, Abutaha N, Ebaid H, Baabbad M et al. Characteristics, Chemical Compositions and Biological Activities of Propolis from Al-Bahah, Saudi Arabia. Sci. Rep. 2017, No. October 2016, 1-13.
Wang C, Wang YX, Yu NQ, Hu D, Wang XY, Chen XG et al. Brazilian Green Propolis Extract Synergizes with Protoporphyrin IX-Mediated Photodynamic Therapy via Enhancement of Intracellular Accumulation of Protoporphyrin IX and Attenuation of NF-κB and COX-2. Molecules 2017, 1-13.
Zabaiou N, Fouache A, Trousson A, Baron S, Zellagui A, Lahouel M et al. Biological Properties of Propolis Extracts : Something New from an Ancient Product. Chem. Phys. Lipids .2017, 1-9.
Chao SC, Young DG. Screening for inhibitory activity of essential oils ou selected bacteria, fungi and viruses. Journal Essentials Oil Research. 2000. 12(4):630-49