Castro-Escarpulli G, Aguilera-Arreola MG, Hernández-Rodríguez CH, Arteaga- Garibay RI, Carmona-Martínez AA, Pérez-Valdespino A, Giono-Cerezo S, Figueras SMJ, Aparicio OG

Language: Spanish
References: 24
Page: 11-18
PDF size: 222.41 Kb.

ABSTRACT

Recently, Aeromonas species have emerged as a public hazard to human population, since cause intestinal and extraintestinal infections. The aim of this study was to identify genetically Aeromonas strains obtained from different sources. 151 strains of Aeromonas were isolated from clinical simples (42), from water (27), and from frozen fish (82) that were presumptively identified by biochemical tests as Aeromonas. Using the automatic method Vitek of bioMérieux carried out complementary biochemical identification. The results presented five species, been A. hydrophila the most frecuent with 50 strains (33%), followed by A. caviae with 31(20%), A. veronii bt sobria with 30 19.8%), A. salmonicida with 29(19.2%), and A. eucrenophila with 11 (7.2%). All the strains were genetic identified by 16S-rDNA RFLP. The molecular method revealed that only 135 out of the 151 strains corresponded to Aeromonas. The seven species identified: A. salmonicida 38%), A. bestiarum (14%), A. veronii (14%), A. hydrophila 13%) , A. caviae (7%), A. encheleia (2%) species reported for first time in Mexico, and A. media (0.6%). We concluded that efficient Aeromonas identification is complex, and requires genetic support, although this kind of procedures is not available in most of the clinical laboratories in our country..

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