2020, Número 2
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Rev Biomed 2020; 31 (2)
Presencia de bacterias del complejo rojo y Aggregatibacter actinomycetemcomitans en dientes primarios necróticos con absceso periapical en niños de Sinaloa, México
Soto-Sainz JE, Alarcón-Romero P, Gastélum-Rosales G, Castro-Salazar Y, Ramos-Payán R, Romero-Quintana G, Aguilar-Medina M, Ayala-Ham AR, Soto-Barreras U, Silva-Benítez EL
Idioma: Ingles.
Referencias bibliográficas: 38
Paginas: 76-83
Archivo PDF: 314.88 Kb.
RESUMEN
Introducción. La necrosis pulpar es uno de los principales motivos
por los cuales se realizan tratamientos en dentición infantil. El
complejo rojo y
Aggregatibacter actinomycetemcomitans (
A.
actinomycetemcomitans) se han asociado con patología pulpares.
Objetivo. Evaluar la presencia del complejo rojo y
A.
actinomycetemcomitans en placa bacteriana, canal radicular y abscesos
periapical en infantes 3 a 9 años de edad con diagnóstico de necrosis
pulpar.
Materiales y métodos. Las muestras de los tres sitios fueron
preservadas y los microorganismos fueron identificados por PCR.
Resultados. Veinte seis niños (5.5 ± 1.7 años) fueron evaluados,
92.3 % fueron positivos para una bacteria en por lo menos uno de
los lugares evaluados. El microrganismo más prevalente fue
T.
forsythia (76.9 %) y el menor
T. denticola (11.5 %). Sin embargo,
A.
actinomycetemcomitans no fue encontrada en ninguna de las muestras.
La placa bacteriana fue el lugar con mayor frecuencia de bacterias
(80.7 %,
p ‹ 0.001). Un diagrama simétrico fue realizado dando como
resultado que
T. forsythia se relacionó con el sitio de placa bacteriana,
mientras
T. denticola con fistula y
P. gingivalis con canal radicular.
Conclusiones. Las bacterias del complejo rojo (
P. gingivalis, T.
denticola y
T. forsythia) fueron encontradas en una alta frecuencia
en los tres sitios analizados.
A. actinomycetemcomitans no fue
encontrada en ninguna muestra. Con estos resultados se observa que
dependiendo de la zona anatómica se modifica la presencia de estos
Red microorganismos, conocer esto es esencial para
elegir apropiadas técnicas de irrigación capaces de
inhibir la proliferación bacteriana y así lograr el
éxito clínico.
REFERENCIAS (EN ESTE ARTÍCULO)
Morabito A, Defabianis P. A SEM investigation on pulpal-periodontal connections in primary teeth. ASDC J Dent Child. 1992;59(1):53-7. PubMed PMID: 1537942.
Tavares WL, Neves de Brito LC, Teles RP, Massara ML, Ribeiro Sobrinho AP, Haffajee AD, et al. Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization. International endodontic journal. 2011;44(3):225-35. doi: 10.1111/j.1365-2591.2010.01805.x. PubMed PMID: 21083570; PubMed Central PMCID: PMCPMC3177302.
Siqueira JF, Jr., Rocas IN, Alves FR, Silva MG. Bacteria in the apical root canal of teeth with primary apical periodontitis. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 2009;107(5):721-6. doi: 10.1016/j.tripleo.2009.01.042. PubMed PMID: 19426923.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. Journal of clinical periodontology. 1998;25(2):134-44. doi: 10.1111/j.1600-051x.1998.tb02419.x. PubMed PMID: 9495612.
da Silva-Boghossian CM, do Souto RM, Luiz RR, Colombo AP. Association of red complex, A. actinomycetemcomitans and non-oral bacteria with periodontal diseases. Archives of oral biology. 2011;56(9):899-906. doi: 10.1016/j. archoralbio.2011.02.009. PubMed PMID: 21397893.
Gomes GB, Sarkis-Onofre R, Bonow ML, Etges A, Jacinto RC. An investigation of the presence of specific anaerobic species in necrotic primary teeth. Brazilian oral research. 2013;27(2):149-55. PubMed PMID: 23538426.
Yun KH, Lee HS, Nam OH, Moon CY, Lee JH, Choi SC. Analysis of bacterial community profiles of endodontically infected primary teeth using pyrosequencing. Int J Paediatr Dent. 2017;27(1):56-65. doi: 10.1111/ipd.12226. PubMed PMID: 26872127.
Nelson-Filho P, Ruviere DB, de Queiroz AM, de Paula- Silva FWG, Silva R, Lucisano MP, et al. Comparative Molecular Analysis of Gram-Negative Bacteria in Primary Teeth with Irreversible Pulpitis or Periapical Pathology. Pediatric dentistry. 2018;40(4):259-64. PubMed PMID: 30345964.
Guven Y, Ustun N, Aksakal SD, Topcuoglu N, Aktoren O, Kulekci G. Assessment of the endodontic microbiota of abscessed primary teeth using microarray technology. Indian J Dent Res. 2018;29(6):781-6. doi: 10.4103/ijdr. IJDR_19_18. PubMed PMID: 30589008.
Rocas IN, Siqueira JF, Jr., Debelian GJ. Analysis of symptomatic and asymptomatic primary root canal infections in adult Norwegian patients. Journal of endodontics. 2011;37(9):1206-12. doi: 10.1016/j. joen.2011.05.026. PubMed PMID: 21846535.
Sakamoto M, Rocas IN, Siqueira JF, Jr., Benno Y. Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol. 2006;21(2):112-22. doi: 10.1111/j.1399- 302X.2006.00270.x. PubMed PMID: 16476021.
Sassone LM, Fidel RA, Faveri M, Figueiredo L, Fidel SR, Feres M. A microbiological profile of unexposed and exposed pulp space of primary endodontic infections by checkerboard DNA-DNA hybridization. Journal of endodontics. 2012;38(7):889-93. doi: 10.1016/j. joen.2012.03.021. PubMed PMID: 22703649.
Pourhajibagher M, Ghorbanzadeh R, Bahador A. Culturedependent approaches to explore the prevalence of root canal pathogens from endodontic infections. Brazilian oral research. 2017;31:e108. doi: 10.1590/1807- 3107bor-2017.vol31.0108. PubMed PMID: 29267669.
Loyola-Rodriguez JP, Garcia-Cortes JO, Martinez- Martinez RE, Patino-Marin N, Martinez-Castanon GA, Zavala-Alonso NV, et al. Molecular identification and antibiotic resistant bacteria isolated from primary dentition infections. Australian dental journal. 2014;59(4):497-503. doi: 10.1111/adj.12213. PubMed PMID: 25091293.
da Silva LA, Nelson-Filho P, Faria G, de Souza- Gugelmin MC, Ito IY. Bacterial profile in primary teeth with necrotic pulp and periapical lesions. Brazilian dental journal. 2006;17(2):144-8. PubMed PMID: 16924343.
Ruviere DB, Leonardo MR, da Silva LA, Ito IY, Nelson- Filho P. Assessment of the microbiota in root canals of human primary teeth by checkerboard DNA-DNA hybridization. J Dent Child (Chic). 2007;74(2):118-23. PubMed PMID: 18477431.
Toyoshima Y, Fukushima H, Inoue JI, Sasaki Y, Yamamoto K, Katao H, et al. [A bacteriological study of periapical pathosis on deciduous teeth]. Shoni Shikagaku Zasshi. 1988;26(3):449-58. PubMed PMID: 3272472.
Fabris AS, Nakano V, Avila-Campos MJ. Bacteriological analysis of necrotic pulp and fistulae in primary teeth. Journal of applied oral science : revista FOB. 2014;22(2):118-24. doi: 10.1590/1678-775720130358. PubMed PMID: 24676582; PubMed Central PMCID: PMCPMC3956403.
Cogulu D, Uzel A, Oncag O, Eronat C. PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 2008;106(3):443-9. doi: 10.1016/j.tripleo.2008.03.004. PubMed PMID: 18547832.
Pazelli LC, Freitas AC, Ito IY, Souza-Gugelmin MC, Medeiros AS, Nelson-Filho P. Prevalence of microorganisms in root canals of human deciduous teeth with necrotic pulp and chronic periapical lesions. Pesqui Odontol Bras. 2003;17(4):367-71. doi: 10.1590/s1517- 74912003000400013. PubMed PMID: 15107921.
Shang JJ, Yang QB, Zhao HY, Cai S, Zhou Y, Sun Z. Preliminary molecular analysis of bacterial composition in periapical lesions with primary endodontic infections of deciduous teeth. Chinese medical journal. 2013;126(16):3112-7. PubMed PMID: 23981622.
Bijoor RR, Kohli K. Contemporary space maintenance for the pediatric patient. N Y State Dent J. 2005;71(2):32- 5. PubMed PMID: 15887467.
Soto-Barreras U, Olvera-Rubio JO, Loyola-Rodriguez JP, Reyes-Macias JF, Martinez-Martinez RE, Patino- Marin N, et al. Peripheral arterial disease associated with caries and periodontal disease. Journal of periodontology. 2013;84(4):486-94. doi: 10.1902/jop.2012.120051. PubMed PMID: 22680302.
Tran SD, Rudney JD. Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. Journal of clinical microbiology. 1996;34(11):2674-8. PubMed PMID: 8897163; PubMed Central PMCID: PMCPMC229384.
Ashimoto A, Chen C, Bakker I, Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol. 1996;11(4):266-73. doi: 10.1111/j.1399-302x.1996. tb00180.x. PubMed PMID: 9002880.
Suzuki N, Nakano Y, Yoshida Y, Ikeda D, Koga T. Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR. Journal of clinical microbiology. 2001;39(5):2002-5. doi: 10.1128/ JCM.39.5.2002-2005.2001. PubMed PMID: 11326035; PubMed Central PMCID: PMCPMC88070.
Martinez-Martinez RE, Abud-Mendoza C, Patino-Marin N, Rizo-Rodriguez JC, Little JW, Loyola-Rodriguez JP. Detection of periodontal bacterial DNA in serum and synovial fluid in refractory rheumatoid arthritis patients. Journal of clinical periodontology. 2009;36(12):1004- 10. doi: 10.1111/j.1600-051X.2009.01496.x. PubMed PMID: 19929953.
Botero JE, Contreras A, Lafaurie G, Jaramillo A, Betancourt M, Arce RM. Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. Journal of periodontology. 2007;78(4):696-704. doi: 10.1902/ jop.2007.060129. PubMed PMID: 17397318.
Souto R, Colombo AP. Prevalence of Enterococcus faecalis in subgingival biofilm and saliva of subjects with chronic periodontal infection. Archives of oral biology. 2008;53(2):155-60. doi: 10.1016/j. archoralbio.2007.08.004. PubMed PMID: 17897617.
Souto R, Colombo AP. Detection of Helicobacter pylori by polymerase chain reaction in the subgingival biofilm and saliva of non-dyspeptic periodontal patients. Journal of periodontology. 2008;79(1):97-103. doi: 10.1902/ jop.2008.070241. PubMed PMID: 18166098.
Cavrini F, Pirani C, Foschi F, Montebugnoli L, Sambri V, Prati C. Detection of Treponema denticola in root canal systems in primary and secondary endodontic infections. A correlation with clinical symptoms. New Microbiol. 2008;31(1):67-73. PubMed PMID: 18437843.
Sato T, Hoshino E, Uematsu H, Noda T. Predominant Obligate Anaerobes in Necrotic Pulps of Human Deciduous Teeth. Microbial Ecology in Health and Disease. 1993;6(6):269-75. doi: 10.3109/08910609309141335.
Ito IY, Junior FM, Paula-Silva FW, Da Silva LA, Leonardo MR, Nelson-Filho P. Microbial culture and checkerboard DNA-DNA hybridization assessment of bacteria in root canals of primary teeth pre- and post-endodontic therapy with a calcium hydroxide/ chlorhexidine paste. Int J Paediatr Dent. 2011;21(5):353- 60. doi: 10.1111/j.1365-263X.2011.01131.x. PubMed PMID: 21481034.
Yang QB, Fan LN, Shi Q. Polymerase chain reactiondenaturing gradient gel electrophoresis, cloning, and sequence analysis of bacteria associated with acute periapical abscesses in children. Journal of endodontics. 2010;36(2):218-23. doi: 10.1016/j.joen.2009.11.001. PubMed PMID: 20113778.
Braga MM, Martignon S, Ekstrand KR, Ricketts DN, Imparato JC, Mendes FM. Parameters associated with active caries lesions assessed by two different visual scoring systems on occlusal surfaces of primary molars - a multilevel approach. Community Dent Oral Epidemiol. 2010;38(6):549-58. doi: 10.1111/j.1600- 0528.2010.00567.x. PubMed PMID: 20735448.
Conrads G, Mutters R, Fischer J, Brauner A, Lutticken R, Lampert F. PCR reaction and dot-blot hybridization to monitor the distribution of oral pathogens within plaque samples of periodontally healthy individuals. Journal of periodontology. 1996;67(10):994-1003. doi: 10.1902/ jop.1996.67.10.994. PubMed PMID: 8910839.
Papaioannou W, Gizani S, Haffajee AD, Quirynen M, Mamai-Homata E, Papagiannoulis L. The microbiota on different oral surfaces in healthy children. Oral Microbiol Immunol. 2009;24(3):183-9. doi: 10.1111/j.1399- 302X.2008.00493.x. PubMed PMID: 19416446.
Kimura S, Ooshima T, Takiguchi M, Sasaki Y, Amano A, Morisaki I, et al. Periodontopathic bacterial infection in childhood. Journal of periodontology. 2002;73(1):20- 6. doi: 10.1902/jop.2002.73.1.20. PubMed PMID: 11846195.