2005, Número 3
<< Anterior Siguiente >>
Rev Biomed 2005; 16 (3)
Método rápido para detección de bacteriuria en examen microscópico de orina no centrifugada.
Luján-Roca DA, Pajuelo-Camacho GR
Idioma: Español
Referencias bibliográficas: 53
Paginas: 169-174
Archivo PDF: 57.89 Kb.
RESUMEN
Objetivo. Evaluar la presencia de bacterias en la examinación microscópica de orina no centrifugada como indicador de bacteriuria significativa.
Material y métodos. Se procesaron 182 muestras de orina, tomando 30 μL de cada una y procediendo con una tinción Gram para la examinación microscópica, cada muestra tuvo su urocultivo respectivo.
Resultados. Comparados con un cultivo ≥ 105 ufc/mL se obtuvieron valores de sensibilidad de 93.6% y especificidad de 89.4%.
Conclusiones. Se concluye que la técnica con asa de siembra tomando 30 μL de orina no centrifugada puede ser usada para un diagnóstico presuntivo de infección de tracto urinario en la práctica bacteriológica.
REFERENCIAS (EN ESTE ARTÍCULO)
1.- Kass EH. Bacteriuria and diagnosis of infections of the urinary tract. Arch Intern Med 1957; 100: 709-14.
2.- Platt R. Quantitative definition of bacteriuria. Amer J Med 1983; 75: 44-52.
3.- Fairley K, Carson N, Gutch R, Leighton P, Grounds A, Laird E, et al. Site of infection in acute urinary tract infection in general practice. Lancet 1971; 2: 615- 8.
4.- Ronald A, Boutros P, Mourtada H. Bacteriuria localization and response to single-dose therapy in woman. JAMA 1976; 235: 1854-6.
5.- Boutros P, Mourtada H, Ronald A. Urinary infection localization. Am J Obstet Gynecol 1972; 112: 379-81.
6.- Jodal U, Lindberg U. Guideline for management of children with urinary tract infection and vesico-ureteric reflux. Recommendations from a Swedish state-of-the-art conference. Acta Paediatr 1999; 431 (Suppl): 87-9.
7.- Norman DC, Yamamura R, Yoshikawa T. Pyuria: its predictive value of asymptomatic bacteriuria in ambulatory elderly men. J Urol 1986; 135: 520-2.
8.- Kolbeck J, Padgett R, Estevez E, Harrell L. Bioluminiscence screening for bacteriuria. J Clin Microbiol 1985; 21: 527-30.
9.- Saito A, Kawada Y. Reliability of pyuria detection method. Infection 1994; 22(suppl 1): 36-7.
10.- Hoberman A, Wald E. Pyuria and bacteriuria in urine specimens obtained by catheter from young children with fever. J Pediatr 1994; 124: 513-9.
11.- Pezzlo M, Ige V, Woolard A, Peterson E, Maza de la L. Rapid bioluminescence method for screening. J Clin Microbiol 1989; 27: 716-20.
12.- Berger SA, Bogokowsky B, Block C. Rapid screening of urine for bacteria and cells by using a catalase reagent. J Clin Microbiol 1990; 28: 1066-7.
13.- Pezzlo M, Ámsterdam D, Anhalt J, Lawrence T, Stratton N, Vetter E, et al. Detection of bacteriuria and pyuria by URISCREEN, a rapid enzymatic screening test. J Clin Microbiol 1992; 30: 680-4.
14.- Hoberman A, Wald E, Penchasky L, Reynolds E, Young S. Enhanced urinalysis as a screening test for urinary tract infection. Pediatrics 1993; 91: 1196-9.
15.- Virella G. Microbiology and infectious diseases. 3th edition. Maryland: Williams and Wilkins; 1997.
16.- Gorelick M, Shaw K. Screenings tests for urinary tract infection in children: a meta-analysis. Pediatrics 1999; 104: 104-10.
17.- Huicho L, Campos-Sanchez M, Alamo C. Metaanalysis of urine screening tests for de-termining the risk of urinary tract infection in children. Pediatr Infect Dis 2002; 21: 1-11.
18.- Deville W, Yzermans J, Duijn van J, Bezemer P, Wind van der D, Bouter L. The urine dipsticks test useful to rule out infections. A meta-analysis of the accuracy. Br Med Clin Urology 2004; 4: 4-17.
19.- Dayan P, Bennett J, Best R, Bregstein J, Levine D, Novick M, et al. Test characteristics of the urine gram stain in infants 60 days of age with fever. Pediatr Emerg Care 2002; 18: 12-4.
20.- Arslan S, Caksen H, Rastgeldi L, Uner A, Oner A, Odabas D. Use of urinary gram stain for detection of urinary tract infection in childhood. Yale J Biol Med 2002;
21.- Benito J, Garcia R, Trebolazabala N, Mintegi S, Vasquez M, Urra E. Gram stain and dipstick as diagnostic methods for urinary tract infection in febrile infants. An Esp Pediatr 2000; 53: 561-6.
22.- Shaw K, Mc Gowan K, Gorelick M, Sanford J. Screening for urinary tract infection in infants in the emergency department: which test is the best? Pediatrics 1998; 101: 1-5.
23.- Appelbaum P, Olmstead C. Evaluation of gram - stain screen and Micro – ID methods for direct identification of Enterobacteriaceae from urines. Med Microbiol Inmunol 1982; 170: 173-84.
24.- Tilton RE, Tilton RC. Automated direct antimicrobial susceptibility testing of microscopically screened urine cultures. J Clin Microbiol 1980; 11: 157-61.
25.- Wu T, Williams E, Koo S, McLowry J. Evaluation of three bacteriuria screening methods in a clinical research hospital. J Clin Microbiol 1985; 21: 796-9.
26.- Cardoso C, Barbosa C, Dias V, Guilhermetti M. Simplified technique for detection of significant bacteriuria by microscopic examination of urine. J Clin Microbiol 1998; 36: