Vidal JE, Enríquez-Rincón F, Giono-Cerezo S, Ribas-Aparicio RM, Figueroa-Arredondo P

Idioma: Ingles.
Referencias bibliográficas: 31
Paginas: 39-47
Archivo PDF: 239.62 Kb.

RESUMEN

Objetivo. Analizar el efecto vacuolizante de cepas de V. cholerae O1 ElTor aisladas de diferente origen geográfico, incluyendo México. Material y métodos. Se realizaron pruebas de hemolisis, vacuolización y citotoxicidad en células Vero, así como PCR, análisis por RFLP y clonación molecular. Resultados. Todas las cepas indujeron el efecto vacuolizante. Las cepas del ribotipo 2, aisladas de las costas del Golfo en Estados Unidos, presentaron títulos altos de vacuolización. El gen hlyA fue amplificado en las nueve cepas mediante PCR, aunque sólo ocho fueron hemolíticas. Se clonó el gen hlyA de una cepa toxigénica (2514-88, ctxAB⁺) y de una cepa no toxigénica aislada en México (CM 91-3, ctxAB^-). El sobrenadante de las clonas recombinantes indujo hemólisis, efecto vacuolizante y citotoxicidad. El RFLP mostró alta similitud del gen hlyA de las cepas estudiadas. Conclusión. El efecto vacuolizante es un fenotipo ampliamente distribuido en cepas epidémicas de V. cholerae O1 biotipo ElTor.

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