medigraphic.com
Instituto Nacional de Salud Pública

2004, Número 1

<< Anterior Siguiente >>

salud publica mex 2004; 46 (1)


Detección de anticuerpos IgA y PCR como primeras opciones en el diagnóstico de infección perinatal por el VIH-1

Basualdo MC, Moran K, Alcántara P, González E, Puentes E, Soler C

Idioma: Español
Referencias bibliográficas: 38
Paginas: 49-55
Archivo PDF: 106.31 Kb.


RESUMEN

Objetivo. Evaluar la sensibilidad y especificidad de la reacción en cadena de la polimerasa y de las pruebas de ELISA e inmunoblot para anticuerpos IgA específicos, como únicos métodos en el diagnóstico de infección perinatal del VIH-1. Material y métodos. Estudio de evaluación comparativa, efectuado entre febrero y octubre de 2001 en la Unidad de Investigación en Retrovirus Humanos de la Universidad Nacional Autónoma de México. Se incluyeron 90 muestras de niños infectados y 153 de no infectados. El cultivo viral fue la prueba de referencia. Se estandarizaron ensayos de ELISA e inmunoblot y la reacción en cadena de la polimerasa para una región conservada del gen gag. Se analizaron los resultados utilizando el paquete informático SPSS 10.0. Resultados. La sensibilidad y especificidad de la prueba de ELISA fueron 61.1 y 90.8%, respectivamente. En el inmunoblot encontramos 82.2 y 95.4%, respectivamente, en tanto que la reacción en cadena de la polimerasa demostró tener sensibilidad de 98.3% y especificidad de 100% con sólo un falso negativo. Conclusiones. Los resultados indican que la realización simultánea de la reacción en cadena de la polimerasa y el inmunoblot para IgA logran sensibilidad y especificidad de 100% y 96%, respectivamente, por lo cual se consideran útiles para el diagnóstico perinatal de VIH-1.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. 1.Newell ML, Peckham C. Risk factors for vertical transmission of HIV-1 and early markers of HIV-I infection in childmen.AIDS 1993;7: S591 -S597.

  2. 2.Van de Perre P. Postnatal transmission of human immunodeficiency virus type I:The breast-feeding dilemma.Am J Obstet Gynecol 1995; 173:483-487.

  3. 3.Committee on Pediatric AIDS. Human milk, breastfeeding and transmission of human immunodeficiency virus in the United States. Pediatrics 1995; 96:977-979.

  4. 4.Schdpbach J,Tomasik Z,Jends j, Bóni j, Seger R, Kind Ch et al. IgG, IgM and 4g.A response to HIV ir Infants born to H1V-1 infected mothers.] A.cquir immune Detc Syndr 1994: 7:421-427.

  5. 5.European Collaborative Study. Mother-to-child transmision of HIV infection. Lancet 1988; ii: 1039-1042.

  6. 6.Moodley D, Bobat RA, Coutsoudis A, Coovadia HM. Predicting perinatal human immunodeficiency virus infection by antibody patterns. Pediatr Infect Dis J 1995; 14: 850-852.

  7. 7.Desrosiers RC.Virus purification, preparation of infectious virus stock, and virus storage. En:Aldovini A,Walker B, Ed.Techniques in HIV research. Nueva York (NY): Stockton Press; 1990: 121-127.

  8. 8.Livingston RA, Hutton N, Halsey NA, Kline RL, Joyner M, QuinnTC. Human immunodeficiency virus-specific IgA in infants born to human immunodeficiency virus-seropositive women.Arch PediatrAdolesc Med 1995; 149: 503-507.

  9. 9.Clerici M, Levin JM, Kessler HA, Harris A, Berzofsky JA Landay AL et al. HIV-specific T-helper activity in seronegative health care workers exposed to contaminated blood. JAMA 1994; 271: 42-46.

  10. 10.Riegelman RK, Hirsch RP. Cómo estudiar un estudio y probar una prueba: lectura crítica de la literatura médica. 2a. ed.Washington, DC: Organización Panamericana de la Salud; 1992; Publicación Científica No. 531: 112-122.

  11. Kline MW, Lewis DE, Hollinger FB, Reuben JM, Hanson C, Kozinetz CA et al. A comparative study of human immunodeficiency virus culture, poymerase chain reaction and anti-human immunodeficiency virus immunoglobulin A antibody detection in the diagnosis during early infancy of vertically acquired human immunodeficiency virus infection. Pediatr Infect Dis J 1994; 13:90-94.

  12. Quinn TC, Kline RL, Halsey N, Hutton N, Ruff A, Butz A et al. Early diagnosis of perinatal HIV infection by detection of viral-specific IgA antibodies. JAMA 1991; 266: 3439-3442.

  13. 13.McIntosh K, Comeau AM,Wara D, Díaz C, Landesman S, Pitt J et al. The utility of IgA antibody to human immunodeficiency virus type I in early diagnosis of vertical transmitted infection.Arch PediatrAdolesc Med 1996;150:598-602.

  14. 14.Report of consensus workshop on early diagnosis of HIV infection in infants: Siena, Italy. J Acquir Immune Defic Syndr 1992, 5:1 169-1 178.

  15. 15.Henrard D, Fauvel J, Samson G, Delege M, Boucher C and Hankins J. Ontogeny of the humoral immune response to humanimmunodeficiency virus type I in infants.) Infect Dis 1993;168:288-291.

  16. 16.Martin NL, Levy JA, Legg H,Weintrub PS, Cowan MJ,Wara D. Detection of infection with human immunodeficiency virus (HIV) type 1 in infants by an anti-HIV immunoglobulin A assay using recombinant proteins.J Pediatr 1991;118:354-358.

  17. I7. Weiblen BJ, Lee FK, Cooper ER, Landesman S, McIntosh K, Harris JA et al. Early diagnosis of HIV infection in infants by detection of IgA HIV antibodies. Lancet 1990; 335:988-990.

  18. Newell ML, Loveday C, Dunn D, Kaye S,Tedder R, Peckham C et al. Use of Polymerase chain reaction and quantitative antibody tests in children born to human immunodeficiency virus-I infected mothers.J Med Virol 1995; 47:330-335.

  19. Wiznia AA, Lambert G, Dobrosyzcki J, Porricolo M, Chelyapov N, Israeli V et al. Virologic, immunologic and clinical evaluation of human immunodeficiency virus antibody status of symptom-free children born to infected mothers. J Pediatr 1994;125:352-355.

  20. Newell ML, Peckham C. Risk factors for vertical transmission of HIV-1 and early markers of HIV-1 infection in children. AIDS 1993;7: S591-S597.

  21. Van de Perre P. Postnatal transmission of human immunodeficiency virus type 1: The breast-feeding dilemma. Am J Obstet Gynecol 1995; 173:483-487.

  22. Committee on Pediatric AIDS. Human milk, breastfeeding and transmission of human immunodeficiency virus in the United States. Pediatrics 1995; 96:977-979.

  23. Schdpbach J, Tomasik Z, Jends J, Bóni J, Seger R, Kind Ch et al. IgG, IgM and IgA response to HIV in Infants born to HIV-1 infected mothers. J Acquir Immune Defic Syndr 1994:7:421-427.

  24. European Collaborative Study. Mother-to-child transmision of HIV infection. Lancet 1988; ii: 1039-1042.

  25. Moodley D, Bobat RA, Coutsoudis A, Coovadia HM. Predicting perinatal human immunodeficiency virus infection by antibody patterns. Pediatr Infect Dis J 1995; 14: 850-852.

  26. Desrosiers RC. Virus purification, preparation of infectious virus stock, and virus storage. En: Aldovini A, Walker B, Ed. Techniques in HIV research. Nueva York (NY): Stockton Press; 1990: 121-127.

  27. Livingston RA, Hutton N, Halsey NA, Kline RL, Joyner M, Quinn TC. Human immunodeficiency virus-specific IgA in infants born to human immunodeficiency virus-seropositive women. Arch Pediatr Adolesc Med 1995; 149: 503-507.

  28. Clerici M, Levin JM, Kessler HA, Harris A, Berzofsky JA, Landay AL et al. HIV-specific T-helper activity in seronegative health care workers exposed to contaminated blood. JAMA 1994; 271: 42-46.

  29. Riegelman RK, Hirsch RP. Cómo estudiar un estudio y probar una prueba: lectura crítica de la literatura médica. 2a. ed. Washington, DC: Organización Panamericana de la Salud; 1992; Publicación Científica No. 531: 112-122.

  30. Kline MW, Lewis DE, Hollinger FB, Reuben JM, Hanson C, Kozinetz CA et al. A comparative study of human immunodeficiency virus culture, poymerase chain reaction and anti-human immunodeficiency virus immunoglobulin A antibody detection in the diagnosis during early infancy of vertically acquired human immunodeficiency virus infection. Pediatr Infect Dis J 1994; 13:90-94.

  31. Quinn TC, Kline RL, Halsey N, Hutton N, Ruff A, Butz A et al. Early diagnosis of perinatal HIV infection by detection of viral-specific IgA antibodies. JAMA 1991; 266: 3439-3442.

  32. McIntosh K, Comeau AM, Wara D, Díaz C, Landesman S, Pitt J et al. The utility of IgA antibody to human immunodeficiency virus type 1 in early diagnosis of vertical transmitted infection. Arch Pediatr Adolesc Med 1996;150:598-602.

  33. Report of consensus workshop on early diagnosis of HIV infection in infants: Siena, Italy. J Acquir Immune Defic Syndr 1992, 5:1169-1178.

  34. Henrard D, Fauvel J, Samson G, Delege M, Boucher C and Hankins J. Ontogeny of the humoral immune response to humanimmunodeficiency virus type 1 in infants. J Infect Dis 1993;168:288-291.

  35. Martin NL, Levy JA, Legg H, Weintrub PS, Cowan MJ, Wara D. Detection of infection with human immunodeficiency virus (HIV) type 1 in infants by an anti-HIV immunoglobulin A assay using recombinant proteins. J Pediatr 1991;118:354-358.

  36. Weiblen BJ, Lee FK, Cooper ER, Landesman S, McIntosh K, Harris JA et al. Early diagnosis of HIV infection in infants by detection of IgA HIV antibodies. Lancet 1990; 335:988-990.

  37. Newell ML, Loveday C, Dunn D, Kaye S, Tedder R, Peckham C et al. Use of Polymerase chain reaction and quantitative antibody tests in children born to human immunodeficiency virus-1 infected mothers. J Med Virol 1995; 47:330-335.

  38. Wiznia AA, Lambert G, Dobrosyzcki J, Porricolo M, Chelyapov N, Israeli V et al. Virologic, immunologic and clinical evaluation of human immunodeficiency virus antibody status of symptom-free children born to infected mothers. J Pediatr 1994;125:352-355.




2020     |     www.medigraphic.com