medigraphic.com
Centro Universitario de Ciencias de la Salud de la Universidad de Guadalajara y del OPD Hospitales Civiles de Guadalajara

2006, Número 3

<< Anterior Siguiente >>

Inv Salud 2006; 8 (3)


Células madre una esperanza para la terapia celular Medicina regenerativa

Soto GA, Navarro ÁN, Rivas CJD, Koutsaftis A, Tanaka N, Kobayashi N

Idioma: Ingles.
Referencias bibliográficas: 39
Paginas: 165-172
Archivo PDF: 414.88 Kb.


RESUMEN

Pacientes que padecen de órganos dañados y enfermos podrían ser tratados mediante el transplante de órganos. Pero debido a la creciente población añosa existe una severa falta de donadores de órganos que empeora año tras año. Científicos y médicos en el área de la medicina regenerativa y la ingeniería tisular han aplicado los principios del transplante celular, ciencia material y bio-ingeniería para construir substitutos biológicos que restauren y mantengan la funcionalidad normal de tejidos dañados y enfermos. La clonación terapéutica, donde el núcleo de una célula donadora es transferida a un óvulo anucleado para extraer células madre embrionarias pluripotentes, ofrece un recurso potencialmente sin límite para la aplicación en la ingeniería tisular sin reacción inmunológica. También el campo de las células madre está avanzando rápidamente, abriendo nuevas opciones de tratamiento. Este articulo revisa los más recientes avances en la medicina regenerativa y describe las aplicaciones de esta nueva tecnología que ofrece nuevas terapias para pacientes con falla orgánica terminal.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Berry MN, Friend DS. "High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study". J Cell Biol 1969;43:506-520.

  2. Kleinsmith LJ, Pierce GB, Jr. "Multipotentiality of Single Embryonal Carcinoma Cells". Cancer Res 1964;24:1544-1551.

  3. Kobayashi N, Okitsu T, Tanaka N. "Cell choice for bioartificial livers". Keio J Med 2003;52:151-157.

  4. Regaud C. "The alternating rhythm of cellular mitoses and the radiosensitivity of the testis". Int J Radiat Oncol Biol Phys 1977;2:569-570.

  5. Leblond CP. "Classification of Cell Populations on the Basis of Their Proliferative Behavior". Natl Cancer Inst Monogr 1964;14:119-150.

  6. Weissman IL. "Stem cells: units of development, units of regeneration, and units in evolution". Cell 2000;100:157-168.

  7. Evans MJ, Kaufman MH. "Establishment in culture of pluripotential cells from mouse embryos". Nature 1981;292:154-156.

  8. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. "Embryonic stem cell lines derived from human blastocysts". Science 1998;282:1145-1147.

  9. Pera MF, Trounson AO. "Human embryonic stem cells: prospects for development". Development 2004;131:5515-5525.

  10. McNeish J. "Embryonic stem cells in drug discovery". Nat Rev Drug Discov 2004;3:70-80.

  11. Strom TB, Field LJ, Ruediger M. "Allogeneic stem cells, clinical transplantation and the origins of regenerative medicine". Curr Opin Immunol 2002;14:601-605.

  12. Wakayama T, Tabar V, Rodriguez I, Perry AC, Studer L, Mombaerts P. "Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer". Science 2001;292:740-743.

  13. Vassilopoulos G, Wang PR, Russell DW. "Transplanted bone marrow regenerates liver by cell fusion". Nature 2003;422:901-904.

  14. Carpenter MK, Rosler ES, Fisk GJ, Brandenberger R, Ares X, Miura T, Lucero M, et al. "Properties of four human embryonic stem cell lines maintained in a feeder-free culture system". Dev Dyn 2004;229:243-258.

  15. Nichols J, Zevnik B, Anastassiadis K, Niwa H, Klewe-Nebenius D, Chambers I, Scholer H, et al. "Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4". Cell 1998;95:379-391.

  16. Niwa H. "Molecular mechanism to maintain stem cell renewal of ES cells". Cell Struct Funct 2001;26:137-148.

  17. Amit M, Carpenter MK, Inokuma MS, Chiu CP, Harris CP, Waknitz MA, Itskovitz-Eldor J, et al. "Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture". Dev Biol 2000;227:271-278. 18. Sato N, Sanjuan IM, Heke M, Uchida M, Naef F, Brivanlou AH. "Molecular signature of human embryonic stem cells and its comparison with the mouse". Dev Biol 2003;260:404-413.

  18. Xu C, Rosler E, Jiang J, Lebkowski JS, Gold JD, O'Sullivan C, Delavan-Boorsma K, et al. "Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium". Stem Cells 2005;23:315-323.

  19. Martin MJ, Muotri A, Gage F, Varki A. "Human embryonic stem cells express an immunogenic nonhuman sialic acid". Nat Med 2005;11:228-232.

  20. Simon C, Escobedo C, Valbuena D, Genbacev O, Galan A, Krtolica A, Asensi A, et al. "First derivation in Spain of human embryonic stem cell lines: use of long-term cryopreserved embryos and animal-free conditions". Fertil Steril 2005;83:246-249.

  21. Yamamoto Y, Teratani T, Yamamoto H, Quinn G, Murata S, Ikeda R, Kinoshita K, et al. "Recapitulation of in vivo gene expression during hepatic differentiation from murine embryonic stem cells". Hepatology 2005;42:558-567.

  22. Soto-Gutierrez A, Kobayashi N, Rivas-Carrillo JD, Navarro-Alvarez N, Zhao D, Okitsu T, Noguchi H, Basma H, Tabata Y, Chen Y, Tanaka K, Narushima M, Miki A, Ueda T, Jun HS, Tanaka N, Lebkowski J, Yoon YW & Fox IJ. "Reversal of mouse hepatic failure using an implanted liver-assist device containing ES cell-derived hepatocytes". Nature Biotech 2006; (In press).

  23. Bagutti C, Wobus AM, Fassler R, Watt FM. “Differentiation of embryonal stem cells into keratinocytes: comparison of wild-type and beta 1 integrin-deficient cells”. Dev Biol 1996;179:184-196.

  24. Coraux C, Hilmi C, Rouleau M, Spadafora A, Hinnrasky J, Ortonne JP, Dani C, et al. "Reconstituted skin from murine embryonic stem cells". Curr Biol 2003;13:849-853.

  25. Fraichard A, Chassande O, Bilbaut G, Dehay C, Savatier P, Samarut J. "In vitro differentiation of embryonic stem cells into glial cells and functional neurons". J Cell Sci 1995;108 ( Pt 10):3181-3188.

  26. Goridis C, Rohrer H. "Specification of catecholaminergic and serotonergic neurons". Nat Rev Neurosci 2002;3:531-541.

  27. Angelov DN, Arnhold S, Andressen C, Grabsch H, Puschmann M, Hescheler J, Addicks K. "Temporospatial relationships between macroglia and microglia during in vitro differentiation of murine stem cells". Dev Neurosci 1998;20:42-51.

  28. Ye W, Shimamura K, Rubenstein JL, Hynes MA, Rosenthal A. "FGF and Shh signals control dopaminergic and serotonergic cell fate in the anterior neural plate". Cell 1998;93:755-766.

  29. Studer L, Csete M, Lee SH, Kabbani N, Walikonis J, Wold B, McKay R. "Enhanced proliferation, survival, and dopaminergic differentiation of CNS precursors in lowered oxygen". J Neurosci 2000;20:7377-7383.

  30. Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, et al. "Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease". Nature 2002;418:50-56.

  31. Takagi Y, Takahashi J, Saiki H, Morizane A, Hayashi T, Kishi Y, Fukuda H, et al. "Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model". J Clin Invest 2005;115:102-109.

  32. Boheler KR, Czyz J, Tweedie D, Yang HT, Anisimov SV, Wobus AM. "Differentiation of pluripotent embryonic stem cells into cardiomyocytes". Circ Res 2002;91:189-201.

  33. Kehat I, Kenyagin-Karsenti D, Snir M, Segev H, Amit M, Gepstein A, Livne E, et al. "Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes". J Clin Invest 2001;108:407-414.

  34. Kehat I, Khimovich L, Caspi O, Gepstein A, Shofti R, Arbel G, Huber I, et al. "Electromechanical integration of cardiomyocytes derived from human embryonic stem cells". Nat Biotechnol 2004;22:1282-1289.

  35. Xue T, Cho HC, Akar FG, Tsang SY, Jones SP, Marban E, Tomaselli GF, et al. «Functional integration of electrically active cardiac derivatives from genetically engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: insights into the development of cell-based pacemakers». Circulation 2005;111:11-20.

  36. Wiles MV, Keller G. "Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture". Development 1991;111:259-267.

  37. Rideout WM, 3rd, Hochedlinger K, Kyba M, Daley GQ, Jaenisch R. "Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy". Cell 2002;109:17-27.

  38. Odorico JS, Kaufman DS, Thomson JA. "Multilineage differentiation from human embryonic stem cell lines". Stem Cells 2001;19:193- 204.

  39. Couzin J, Kaiser J. Gene therapy. "As Gelsinger case ends, gene therapy suffers another blow". Science 2005;307:1028.




2020     |     www.medigraphic.com