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2002, Number 2

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Bioquimia 2002; 27 (2)

Ecuations to eliminate interference of hemolised, icteric and hiperglycemic sera in rutine determinations of chemical chemistry

Sánchez-Rodríguez MA, Colunga-Reyes R, Cedillo-Martínez MP

Language: Spanish
References: 29
Page: 46-52
PDF size: 179.92 Kb.


ABSTRACT

Background: Specimens hyperglycemic, icteric or with hemolysis can produce interference at time of analysis, because the methods used in clinical chemistry determinations are based on spectrophotometric techniques.
Objective: To quantify the interference in vitro produced by different concentrations of glucose, bilirubin and hemoglobinin the usual techniques to analyze the serum levels of urea, creatinine, uric acid and cholesterol by manual and analyzer system procedures.
Methods: We collected 300 mL of free hemolysis, bilirubin,and lipemia serum. We measured glucose, urea, creatinine, uricacid, cholesterol, hemoglobin and total bilirubin serum concentration by both procedures. We add IFCC recommendedconcentrations of hemoglobin, bilirubin, and glucose, and wegot the interference point for each component using correlationgraphics and lineal equations to correct.
Results: Bilirubin serum concentration when reaches 3.0 mg/dL (51.3µmol/L) produces interference, except for cholesterol measurement. Hemoglobin serum concentration when reaches 1.0 g/L (0.062 mmol/L) produces interference in uric acid, creatinine, and cholesterol measurements. Serum glucose does not produce any interference. The regression model for uric acidand bilirubin is not significant.
Conclusions: The hemolysed and icteric specimens cause interference in most of the measurements, mainly from manual procedures. The equations obtained could be used to calculatethe analytes without interference.


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