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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2021, Number 2

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Rev Biomed 2021; 32 (2)

Detection of the polymorphism -174 G›C of the IL-6 gene in patients with periodontitis

Ruiz-García L, Cruz-Rubio JA, Carrillo-Ávila BA, Sauri-Esquivel E, Valencia-Pacheco G, Valadez-González N, Martínez-Aguilar VM

Language: Spanish
References: 37
Page: 78-86
PDF size: 519.17 Kb.


ABSTRACT

Background. Among the risk factors for periodontitis, genetic factors have been described. Interleukin-6 (IL-6) is a cytokine involved in the infl ammatory process and periodontal destruction. Polymorphisms in the IL-6 gene may explain the risk of suff ering the disease in certain populations.
Objective. To determine the association of the gene g.-174 G›C polymorphism with stage II grade B periodontitis.
Material and methods. Case-control study. The genotypic and allelic frequencies of g.-174 G›C were analyzed in 102 subjects, 52 cases with periodontitis stage II grade B and 50 controls, by quantitative PCR (qPCR). The association of the polymorphism with the risk for periodontitis was determined by univariate analysis.
Results. Genotypic frequencies for the g.-174 G›C polymorphism were determined. The wild homozygous genotype GG was the most prevalent (90.4 %) in the cases with respect to the controls (78 %), as well as the allele G (95.2 % and 88 % respectively). The homozygous mutated CC genotype was observed decreased in controls and absent in cases. The analysis showed no association of the g.-174 G/C polymorphism with periodontitis stage II grade B.
Discussion. Walker et al,. (2017) obtained similar results to ours, with the studied population being African-American from western North Carolina.
Conclusion. No association was observed between the g.-174 G/C polymorphism and periodontitis stage II grade B. Likewise, a low frequency of the homozygous mutated CC genotype has been reported, which was found in 2% of the controls and absent in the cases studied.


REFERENCES

  1. Ocampo PV. Enfoque salubrista de la enfermedad periodontal. Rev Iberoam CIencias [Internet]. 2015;2(4):179–89. Disponible en: www.reibci.org

  2. Herrera D., Figuero E., Shapira L., Jin L. SM. Diagnóstico y Tratamiento Periodontal. Rev científica la Soc Española Periodoncia. 2018;(11):1–24.

  3. Papapanou PN, Susin C. Periodontitis epidemiology: is periodontitis under-recognized, over-diagnosed, or both? Periodontol 2000. 2017;75(1):45–51.

  4. Pardo Romero FF, Hernandez LJ. Enfermedad periodontal: enfoques epidemiológicos para su análisis como problema de salud pública. Rev Salud Publica. 2018;20(2):258–64.

  5. Lomelí G, Rodríguez K, Mejía A. Resultados 2018 del Sistema de vigilancia epidemiológica de patologías bucales. Secr Salud, SIVEPAB. 2018;80.

  6. Arróniz S, Furuya A, Gómez A, Garzón J, Redondo C, Martínez J. Proteína C Reactiva de alta especificidad como marcador de la enfermedad periodontal. Oral. 2013;(46):1026–9.

  7. Peña S, Calzado M, González M, Cordero S, Azahares H. Patógenos periodontales y sus relaciones con enfermedades sistémicas. MediSan. 2012;16(7):1137– 48.

  8. Carranza F, Newman M, Takei H, Klokkevold P. Periodontología Clínica. 10a ed. México: McGraw- Hill Interamericana; 2017. 1552 p.

  9. Herrera M, Guerrero C, Martínez V. Participación de la IL-17 en la periodontitis crónica y agresiva. Rev Mex Periodontol. 2013;IV(2):73–7.

  10. Research S and therapy committee. Position Paper: Diagnosis of Periodontal Diseases. J Periodontol [Internet]. agosto de 2003;74(8):1237–47. Disponible en: http://doi.wiley.com/10.1902/jop.2003.74.8.1237

  11. Nieminen A, Asikainen S, Torkko H, Kari K, Uitto V-J, Saxen L. Value of some laboratory and clinical measurements in the treatment plan for advanced periodontitis. J Clin Periodontol [Internet]. junio de 1996;23(6):572–81. Disponible en: http://doi.wiley. com/10.1111/j.1600-051X.1996.tb01827.x

  12. Nieminen A, Sirén E, Wolf J, Asikainen S. Prognostic criteria for the efficiency of non-surgical periodontal therapy in advanced periodontitis. J Clin Periodontol [Internet]. el 13 de diciembre de 2005;22(2):153–61. Disponible en: http://doi.wiley.com/10.1111/j.1600- 051X.1995.tb00127.x

  13. Kornman KS, di Giovine FS. Genetic Variations in Cytokine Expression: A Risk Factor for Severity of Adult Periodontitis. Ann Periodontol [Internet]. julio de 1998;3(1):327–38. Disponible en: http://doi.wiley. com/10.1902/annals.1998.3.1.327

  14. Nagarajan R, Miller CS, Dawson D, Al-Sabbagh M, Ebersole JL. Cross-talk between clinical and hostresponse parameters of periodontitis in smokers. J Periodontal Res [Internet]. junio de 2017;52(3):342– 52. Disponible en: http://doi.wiley.com/10.1111/ jre.12397

  15. Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol [Internet]. junio de 2018;89:S173–82. Disponible en: http://doi. wiley.com/10.1002/JPER.17-0721

  16. National Library of Medicine. dbSNP [Internet]. https://www.ncbi.nlm.nih.gov/snp/rs1800795. 2020 [citado el 11 de septiembre de 2020]. Disponible en: https://www.ncbi.nlm.nih.gov/snp/rs1800795

  17. Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today [Internet]. enero de 1990;11:443–9. Disponible en: https://linkinghub.elsevier.com/retrieve/ pii/0167569990901737

  18. Bertazzolo N, Punzi L, Stefani MP, Cesaro G, Pianon M, Finco B, et al. Interrelationships between interleukin (IL)-1, IL-6 and IL-8 in synovial fluid of various arthropathies. Agents Actions [Internet]. marzo de 1994;41(1–2):90–2. Disponible en: http:// link.springer.com/10.1007/BF01986402

  19. Dongari-Bagtzoglou AI, Ebersole JL. Increased Presence of Interleukin-6 (IL-6) and IL-8 Secreting Fibroblast Subpopulations in Adult Periodontitis. J Periodontol [Internet]. agosto de 1998;69(8):899– 910. Disponible en: http://doi.wiley.com/10.1902/ jop.1998.69.8.899

  20. Giannopoulou C, Kamma JJ, Mombelli A. Effect of inflammation, smoking and stress on gingival crevicular fluid cytokine level. J Clin Periodontol [Internet]. febrero de 2003;30(2):145–53. Disponible en: http://doi.wiley.com/10.1902/jop.1998.69.8.899

  21. Kern PA, Ranganathan S, Li C, Wood L, Ranganathan G. Adipose tissue tumor necrosis factor and interleukin-6 expression in human obesity and insulin resistance. Am J Physiol Metab [Internet]. el 1 de mayo de 2001;280(5):E745–51. Disponible en: https://www. physiology.org/doi/10.1152/ajpendo.2001.280.5.E745

  22. Vignal A, Milan D, SanCristobal M, Eggen A. A review on SNP and other types of molecular markers and their use in animal genetics. Genet Sel Evol [Internet]. el 15 de junio de 2002;34(3):275. Disponible en: https://gsejournal.biomedcentral.com/ articles/10.1186/1297-9686-34-3-275

  23. Vasunilashorn S, Finch CE, Crimmins EM, Vikman SA, Stieglitz J, Gurven M, et al. Inflammatory gene variants in the tsimane, an indigenous bolivian population with a high infectious load. Biodemography Soc Biol. 2011;57(1):33–52.

  24. Martínez-Cortés G, Nuño-Arana I, Rubi-Castellanos R, Vilchis-Dorantes G, Luna-Vázquez a, Coral-Vázquez RM, et al. Origin and genetic differentiation of three Native Mexican groups (Purépechas, Triquis and Mayas): contribution of CODIS-STRs to the history of human populations of Mesoamerica. Ann Hum Biol [Internet]. 2010;37(6):801–19. Disponible en: http:// www.ncbi.nlm.nih.gov/pubmed/20515366

  25. Shehab MJ, Abdul-hassan IA. The Association of Interleukin-6 Gene Polymorphism at -174G?C SNP in Iraqi Patients with type 2 Diabetes Mellitus. Elixir Int J Physiol an Anat. 2015;88:3672–6.

  26. Martínez V, Carrillo B, García G, Vera L, Escobar D, Pozos A, et al. Asociación de los polimorfismos CCR5 G59029A y RANTES –28 C/G en pacientes con periodontitis crónica y/o diabetes mellitus tipo 2 en una población del sureste mexicano. Invest Clin. 2018;59(2):135.

  27. Saxena M, Agrawal C, Srivastava N, M. B. Interleukin-6 (IL-6)-597 A/G (rs1800797) & -174 G/C (rs1800795) gene polymorphisms in type 2 diabetes. Indian J Med Res. 2014;140(1):60–8.

  28. Kubaszek A, Pihlajamaki J, Komarovski V, Lindi V, Lindstrom J, Eriksson J, et al. Promoter Polymorphisms of the TNF- (G-308A) and IL-6 (C-174G) Genes Predict the Conversion From Impaired Glucose Tolerance to Type 2 Diabetes: The Finnish Diabetes Prevention Study. Diabetes [Internet]. el 1 de julio de 2003;52(7):1872–6. Disponible en: http:// diabetes.diabetesjournals.org/cgi/doi/10.2337/ diabetes.52.7.1872

  29. Bouhaha R, Baroudi T, Ennafaa H, Vaillant E, Abid H, Sassi R, et al. Study of TNF? -308G/A and IL6 -174G/C polymorphisms in type 2 diabetes and obesity risk in the Tunisian population. Clin Biochem [Internet]. abril de 2010;43(6):549–52. Disponible en: https://linkinghub. elsevier.com/retrieve/pii/S0009912010000330

  30. Papaoikonomou S, Tentolouris N, Tousoulis D, Papadodiannis D, Miliou A, Papageorgiou N, et al. The association of the 174G>C polymorphism of interleukin 6 gene with diabetic nephropathy in patients with type 2 diabetes mellitus. J Diabetes Complications [Internet]. noviembre de 2013;27(6):576–9. Disponible en: https://linkinghub.elsevier.com/retrieve/pii/ S1056872713001475

  31. Song GG, Choi SJ, Ji JD, Lee YH. Association between tumor necrosis factor-? promoter ?308 A/G, ?238 A/G, interleukin-6 ?174 G/C and ?572 G/C polymorphisms and periodontal disease: a meta-analysis. Mol Biol Rep [Internet]. el 9 de agosto de 2013;40(8):5191– 203. Disponible en: http://link.springer.com/10.1007/ s11033-013-2621-4

  32. Nibali L, Madden I, F. F, Chillida L, Heitz-Mayfield L, Brett P, et al. IL6 -174 genotype associated with Aggregatibacter actinomycetemcomitans in Indians. Oral Dis. 2011;17(2):232–7.

  33. Raunio T, Nixdorf M, Knuuttila M, Karttunen R, Vainio O, Tervonen T. The extent of periodontal disease and the IL-6 ?174 genotype as determinants of serum IL-6 level. J Clin Periodontol [Internet]. diciembre de 2007;34(12):1025–30. Disponible en: http://doi.wiley. com/10.1111/j.1600-051X.2007.01151.x

  34. Shao M, Huang P, Cheng R, Hu T. Interleukin-6 polymorphisms modify the risk of periodontitis:a systematic review and meta-analysis. J Zhejiang Univ Sci B [Internet]. el 4 de diciembre de 2009;10(12):920– 7. Disponible en: http://link.springer.com/10.1631/ jzus.B0920279

  35. Gabriela Teixeira F, Mendonça SA, Menezes Oliveira K, Barbosa dos Santos D, Miranda Marques L, Mendonça Amorim M, et al. Interleukin-6 c.-174G>C Polymorphism and Periodontitis in a Brazilian Population. Mol Biol Int [Internet]. 2014;2014:1– 8. Disponible en: https://www.hindawi.com/ archive/2014/490308/

  36. Walker SJ, Van Dyke TE, Rich S, Kornman KS, di Giovine FS, Hart TC. Genetic Polymorphisms of the IL-1? and IL-1? Genes in African-American LJP Patients and an African-American Control Population. J Periodontol [Internet]. mayo de 2000;71(5):723– 8. Disponible en: http://doi.wiley.com/10.1902/ jop.2000.71.5.723

  37. Pociot F, Molvig J, Wogensen L, Worsaae H, Nerup J. A Taql polymorphism in the human interleukin-1? (IL-1?) gene correlates with IL-1? secretion in vitro. Eur J Clin Invest [Internet]. junio de 1992;22(6):396–402. Disponible en: http://doi.wiley. com/10.1111/j.1365-2362.1992.tb01480.x




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