Feitosa AV, Dantas FR, Wanderley CY, Nascimento PWW

Language: Portugués
References: 21
Page: 1-16
PDF size: 322.21 Kb.

ABSTRACT

Introduction: A list of 71 medicinal herbs has been published by the Brazilian Ministry of Health with the purpose of studying and developing medicines obtained from herbs for their potential use in the Unified Health System.
Objective: Evaluate the in vitro antimicrobial activity of extracts from medicinal herbs proposed by the Ministry of Health with a view to their possible use in the Unified Health System to combat oral bacteria.
Methods: Extracts were obtained from the plants selected in the form of essential oil (Eucalyptus globulus, Mentha piperita and Schinus terebinthifolius) or tincture (Erythrina mulungu, Casearia sylvestris and Maytenus ilicifolia), and were tested against Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557) and S. salivarius (ATCC 7073). The agar diffusion test was performed by making wells and adding 50 μL of the extracts. After 48 h in a bacteriological incubator, the inhibition haloes were measured with calipers. Minimum inhibitory concentration and minimum bactericidal concentration were determined by microplate microdilution and depletion technique, respectively. The minimum inhibitory concentration corresponded to the lowest dilution, at which there was no visible bacterial growth. The positive control used was 0.12% chlorhexidine. All the tests were performed in triplicate and analyzed descriptively.
Results: Maytenus ilicifolia extracts exhibited slightly higher inhibition haloes than the other products. Erythrina mulungu had the lowest minimum inhibitory concentration against S. mutans (2.81 mg/mL) among tinctures, and Mentha piperita (9.00) among essential oils. Erythrina and Mentha piperita extracts were the only two displaying minimum bactericidal concentration against the strains used.
Conclusions: The study plant extracts displayed antimicrobial activity against oral microorganisms, particularly against Mentha piperita and Erythrina mulungu.

REFERENCES

1. Brasil. Ministério da Saúde. Secretaria de Atenção à Saúde. Departamento de Atenção Básica. Práticas integrativas e complementares: plantas medicinais e fitoterapia na Atenção Básica. Brasília: Ministério da Saúde; 2012. 2. Batista LM, Valença AMG. A fitoterapia no âmbito da atenção básica no SUS: realidades e perspectivas. Pesq Bras Odontoped Clín Integr. 2012;12(2):293-6.

2. Brasil. Portal da Saúde. Secretaria de Ciência, Tecnologia e Insumos Estratégicos. Departamento de Assistência Farmacêutica e Insumos Estratégicos. Programa Nacional de Plantas Medicinais e Fitoterápicos. Brasília: Ministério da Saúde; 2009.

3. Freires IA, Denny C, Benso B, Alencar SM, Rosalen PL. Antibacterial activity of essential oils and their isolated constituents against cariogenic bacteria: a systematic review. Molecules. 2015;20(4):7329-58.

4. Kumar G, Jalaluddin M, Rout P, Mohanty R, Dileep CL. Emerging trends of herbal care in dentistry. J Clin Diagn Res. 2013;7(8):1827-9.

5. Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou JB. Composition and Antimicrobial Activity of the Essential Oils of Two Origanum Species. J Agric Food Chem. 2001;49(9):4168-70.

6. Lima IO, Oliveira RAG, Lima EO, Farias NMP, Souza EL. Atividade antifúngica de óleos essenciais sobre espécies de Candida. Rev Bras Farmacogn. 2006;16(2):197-201.

7. Karygianni L, Al-Ahmad A, Argyropoulou A, Hellwig E, Anderson AC, Skaltsounis LA. Natural antimicrobials and oral microorganisms: A systematic review on herbal interventions for the eradication of multispecies oral biofilms. Front Microbiol. 2016;6:1529.

8. Freires IA, Alves LA, Ferreira GLS, Jovito VDC, Castro RDD, Cavalcanti AL. A randomized clinical trial of Schinus terebinthifolius mouthwash to treat biofilm-induced gingivitis. Evid Based Complement Alternat Med. 2013:1-8. doi:10.1155/2013/873907

9. Lins R, Vasconcelos FHP, Leite RB, Coelho-Soares RS, Barbosa DN. Avaliação clínica de bochechos com extratos de Aroeira (Schinus terebinthifolius) e Camomila (Matricaria recutita L.) sobre a placa bacteriana e a gengivite. Rev Bras Pl Med. 2013;15(1):112-20.

10. Smolarek PC, Esmerino LA, Chibinski AC, Bortoluzzi MC, Santos EB, Kozlowski VA. In vitro antimicrobial evaluation of toothpastes with natural compounds. Eur J Dent. 2015;9(4):580-6.

11. Scorzoni L, Benaducci T, Almeida AMF, Silva DHS, Bolzani VS, Mendes-Giannini MJS. Comparative study of disk diffusion and microdilution methods for evaluation of antifungal activity of natural compounds against medical yeasts Candida spp and Cryptococcus sp. Rev Ciênc Farm Básica Apl. 2007;28(1):25-34.

12. Ahmad A, Khan A, Samber N, Manzoor N. Antimicrobial activity of Mentha piperita essential oil in combination with silver ions. Synergy 2014;1(2):92-8.

13. Shalayel MHF, Asaad AM, Qureshi MA, Elhussein AB. Anti-bacterial activity of peppermint (Mentha piperita) extracts against some emerging multi-drug resistant human bacterial pathogens. Journal of Herbal Medicine. 2017;7:27-30.

14. Alves LA, Freires IA, Castro RD. Efeito Antibacteriano de Óleos Essenciais sobre Bactérias Formadoras do Biofilme Dentário. Rev Bras Ciênc Saúde. 2011;14(2):57-62.

15. Singh R, Shushni MA, Belkheir A. Antibacterial and antioxidant activities of Mentha piperita L. Arabian Journal of Chemistry. 2015;8(3):322-8.

16. Goldbeck JC, Nascimento JE, Jacob RG, Fiorentini AM, Silva WP. Bioactivity of essential oils from Eucalyptus globulus and Eucalyptus urograndis against planktonic cells and biofilms of Streptococcus mutans. Industrial Crops and Products. 2014;60:304-9.

17. Freires IDA, Alves LA, Jovito VDC, Almeida LDFDD, Castro RDD, Padilha WWN. Atividades antibacteriana e antiaderente in vitro de tinturas de Schinus terebinthinfolius (Aroeira) e Solidago microglossa (Arnica) frente a bactérias formadoras do biofilme dentário. Odontol Clín-Cient. (Online). 2010;9(2):139-43.

18. Jain MK, Yu BZ, Rogers JM, Smith AE, Boger ET, Ostrander RL, et al. Specific competitive inhibitor of secreted phospholipase A 2 from berries of Schinus terebinthifolius. Phytochemistry. 1995;39(3):537-47.

19. Jain P, Ranjan M. Role of herbs in intracanal medicaments. Int J Pharm Bio Sci. 2014;5(3):126-31.

20. Ameni AZ, Latorre OA, Torres LMB, Górniak SL. Toxicity study about a medicinal plant Casearia sylvestris: A contribution to the Brazilian Unified Health System (SUS). Journal of Ethnopharmacology. 2015;175:9-13.

21. Espinosa J, Medeiros LF, Souza AD, Güntzel ARDC, Rücker B, Casali EA et al. Ethanolic extract of Casearia sylvestris Sw exhibits in vitro antioxidant and antimicrobial activities and in vivo hypolipidemic effect in rats. Rev Bras Pl Med. 2015;17(2):49-55.