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2019, Number 1

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Cuba y Salud 2019; 14 (1)

Quantitative immunoelectron microscopy to the study of EGF therapeutic effect on diabetic foot ulcers

González BM, Acosta RN, González PS, Kourí FJ, Berlanga AJ, Falcón CV

Language: Spanish
References: 30
Page: 99-106
PDF size: 1333.75 Kb.


ABSTRACT

Objective: in this work we focused on the methodology used to investigate the differential cellular distribution of a key protein (cellular proliferation nuclear antigen) involved in the signalling induced by under epidermal growth factor.
Development: samples from diabetic foot ulcers were analysed using quantitative IEM. References were obtained from the Pubmed data base. In agreement with an under epidermal growth factor functional failure in the diabetic granulation tissue, cellular proliferation nuclear antigen was scarcely detected in fibroblasts. Interestingly, under epidermal growth factor treatment of diabetic foot ulcers induced an early activation of cellular proliferation nuclear antigen (from 15 min to 60 min) in the nucleus of diabetic foot ulcers fibroblasts. Notably, augmented labeling of cellular proliferation nuclear antigen was observed in mitochondria of fibroblasts at later times following under epidermal growth factor therapy (from 6 hours to 24 hours).
Conclusions: this research demonstrated the value of applying quantitative immunogoldlabeling distributions of cellular compartments to the study of relevant therapeutic intracellular signaling pathways.


REFERENCES

  1. Mayhew TM, Lucocq JM. Developments in cell biology for quantitative immunoelectron microscopy based on thin sections– a review. Histochem Cell Biol. 2008;130:299–313.

  2. Mayhew TM, Lucocq JM, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc. 2002;205:153-64.

  3. Berlanga-Acosta J, Mendoza-Marí Y, Fernández-Mayola M, García-Ojalvo A, Valdés-Pérez C, Savigne-Gutierrez W, et al. Torpid Diabetic Wound Healing: Evidence on the Role of Epigenetic Forces. Int J Diabetes Clin Res. 2015;2:020.

  4. Bobircē F, Mihalache O, Georgescu D, al. e. The new prognostic-therapeutic index for diabetic foot surgery--extended analysis. Chirurgia 2016;111:151-5.

  5. Zhang P, Lu J, Jing Y, al. e. Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis. Ann Med. 2016;49:106-16

  6. International_Diabetes_Federation. Atlas de la Diabetes de la FID. 2017.

  7. Berlanga-Acosta J, López-Saura P, Guillen-Pérez I, Guillen-Nieto G, Acevedo-Castro B, Herrera-Martínez L. Type 2 Diabetes Mellitus (T2DM): Biological Overview from Pathways to Organelles and its Translation toward a Torpid Wound Healing Process. J Diabetes Metab. 2013;4(7):285.

  8. Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes. 2005;54:1615-25.

  9. Groop L, Pociot F. Genetics of diabetes--are we missing the genes or the disease? Mol Cell Endocrinol. 2014;382(1):726-39.

  10. Bagalad BS, Kumar KPM, Puneeth HK. Myofibroblasts: Master of disguise. J Oral Maxillofac Pathol. 2017;21:462-3.

  11. Bochaton-Piallat M-L, Gabbiani G, Hinz B. The myofibroblast in wound healing and fibrosis: answered and unanswered questions. F1000 Research. 2016;5:752.

  12. Gerarduzzi C, Di Battista JA. Myofibroblast repair mechanisms post-inflammatory response: a fibrotic perspective. Inflamm Res. 2017;Doi: 10.1007/s00011-016-1019-x.

  13. Berlanga-Acosta J, Schultz GS, Lopez-Mola E, Guillen-Nieto G, García-Siverio M, Herrera-Martinez L. Glucose Toxic Effects on Granulation Tissue Productive Cells: The Diabetics Impaired Healing. BioMed Res Int. 2013;2013:15.

  14. Falcón-Cama V, Fernández-Mayola M, Mendoza-Mari Y, Acosta-Rivero N, García-Ojalvo A, Bringas-Pérez R, et al. Epidermal Growth Factor based Therapy Promotes Intracellular Trafficking and Accumulation of its Receptor in the Nucleus of Fibroblasts from Diabetic Foot Ulcers. J Diabetic Complications Med. 2016;1(3):111.

  15. Portou MJ, Baker D, Abraham D, Tsui J. The innate immune system, toll-like receptors and dermal wound healing: A review, Vascular Pharmacology Vascular Pharmacol. 2015;doi: 10.1016/j.vph.2015.02.007.

  16. Berlanga-Acosta J. Diabetic lower extremity wounds: the rationale for growth factors-based infiltration treatment. Int Wound J. 2011;8(1742-481X (Electronic)):612-20.

  17. Buckley A, Davidson JM, Kamerath CD, Wolt TB, Woodward SC. Sustained release of epidermal growth factor accelerates wound repair. Proc Natl Acad Sci USA. 1985;82(21):7340-4.

  18. Yu FS, Yin J, Xu K, Huang J. Growth factors and corneal epithelial wound healing. Brain Res Bull. 2010;81(2-3):229-35.

  19. Berlanga-Acosta J, Fernández-Montequín J, Valdés-Pérez C, Savigne-Gutiérrez W, Mendoza-Marí Y, García-Ojalvo A, et al. Diabetic Foot Ulcers and Epidermal Growth Factor: Revisiting the Local Delivery Route for a Successful Outcome. Biomed Res Int. 2017;2017: 2923759.

  20. Naryzhny SN. Proliferating cell nuclear antigen: a proteomics view. Cell Mol Life Sci. 2008;65(23):3789-808.

  21. Park SY, Jeong MS, Han CW, Yu HS, Jang SB. Structural and functional insight into proliferating cell nuclear antigen JMB. 2015.

  22. Stoimenov I, Helleday T. PCNA on the crossroad of cancer. Biochem Soc Trans. 2009;37:605-13.

  23. Mayhew TM. Quantitative immunocytochemistry at the ultrastructural level:a stereology-based approach to molecular nanomorphomics. Cell Tissue Res. 2015;360(1):43-59.

  24. Mayhew TM, Lucocq JM. From gross anatomy to the nanomorphome: stereological tools provide a paradigm for advancing research in quantitative morphomics J Anat. 2015;226:309--21.

  25. Lucocq JM, Mayhew TM, Schwab Y, Steyer AM, Hacker C. Systems biology in 3D space – enter the morphome. Trends Cell Biol. 2015;25(2):59-64.

  26. Mayhew TM. Morphomics: An integral part of systems biology of the human placenta. Placenta. 2015;36:329e40.

  27. Gundersen HJG, Jensen EB, Kieu K, al. e. The efficiency of systematic sampling in stereology reconsidered. J Microsc. 1999;193:199–211.

  28. Mayhew TM. Taking tissue samples from the placenta: an illustration of principles and strategies. Placenta. 2008;29:1–14.

  29. Borjigin G, Yamamoto S, Sugiyama T, Nishiumi T, Suzuki A. Changes in the immunogold electron-microscopic localization of calpain in bovine skeletal muscle induced by conditioning and high-pressure treatment. Biosci Biotechnol Biochem 2006;70:1249– 54.

  30. Schmiedl A, Ochs M, Muhlfeld C, Johnen G, Brasch F. Distribution of surfactant proteins in type II pneumocytes of newborn, 14- day old, and adult rats: an immunoelectron microscopic and stereological study. Histochem Cell Biol 2005;124:465–76.




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