2019, Number 4
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Rev Cub Oftal 2019; 32 (4)
Concordance between different cell counts according to morphologic and morphometric parameters of the corneal endothelium
Guerra AM, Cárdenas DT, Tamargo BTO, Pérez SRG, Cruz ID, Rivera JG
Language: Spanish
References: 24
Page: 1-12
PDF size: 415.85 Kb.
ABSTRACT
Objective: To estimate the morphological and morphometric values of the corneal
endothelium according to the number of cells and evaluate the interobserver concordance for
the different parameters, estimated according to the different cell counts in adults without corneal alterations.
Methods: An observational, descriptive and cross-sectional case series research was carried
out in the Refractive Surgery Service of Ramón Pando Ferrer Cuban Institute of
Ophthalmology during two years of study. After applying the exclusion criteria, the sample
was made up of 200 eyes of 100 adult patients without corneal alterations. Non-contact
endothelial microscopy SP-3000P was performed to identify morphological values
(hexagonality and coefficient of variability) and morphometric values (cell density), as well as
the average corneal cell size according to the number of cells evaluated.
Results: According to the amount of endothelial cells evaluated, there were no significant
differences between morphological and morphometric variables (p>0.05) in both eyes. The
agreement between the different cell counts according to the values of the interclass correlation
coefficients (ICC) were all high. The interobserver concordance and ICCs were also high,
except for hexagonality.
Conclusions: The morphological and morphometric values of the corneal endothelium,
according to the number of cells evaluated, are similar in all cell counts. Good concordance
between the different cell counts studied for the different estimated parameters is
demonstrated.
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