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Medicina & Laboratorio

2017, Number 03-04

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Medicina & Laboratorio 2017; 23 (03-04)

Evaluation of Albert´s staining technique for Corynebacterium spp. in vaginal swabs

Botero-Vélez AM, Ossa-Giraldo AC, Vélez-Restrepo ML, Cuervo-Araque CM, Úsuga-Perilla X

Language: Spanish
References: 38
Page: 179-186
PDF size: 419.65 Kb.


ABSTRACT

Introduction: The detection of Corynebacterium genus in vaginal discharge represents a challenge for the study of its possible pathogenic role in the female genital tract. Gram’s stain is recommended for corynebacteria detection in clinical samples, however, some biological characteristics of these bacteria difficult their identification by this technique. Objetive: to evaluate Albert’s stain performans to identify Corynebacterium spp. in vaginal discharge. Materials and methods: Albert’s stain was evaluated as a diagnostic test for the identification of Corynebacterium spp. in 451 vaginal swabs in comparison to Gram’s stain. Sensitivity, specificity, predictive values, likelihood ratio, as well as Youden’s J index were calculated using Epidat software 3.1 version. Results: Albert’s stain had a sensitivity and specificity of 61.5% and 74.6%, respectively, positive predictive value of 78.3 %, negative predictive value of 56.5 %, positive likelihood ratio of 2.4 and negative likelihood ratio of 0.5, as well as Youden index of 0.4. Conclusions: Albert’s stain did not show better performance than Gram’s stain for identification of corynebacteria in vaginal swabs. Thereforeit is recommended the concomitant use of the Gram and Albert stains for the identification of corynebacteria in vaginal discharge.


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