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ISSN 0123-2576 (Print)
Medicina & Laboratorio

2017, Number 03-04

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Medicina & Laboratorio 2017; 23 (03-04)

Preservation of obtained biopsies by colonoscopy in patients with colorectal cancer for molecular analysis

Uribe-Echeverry PT, Arturo-Arias BL, García-Restrepo N, Betancur-Pérez JF

Language: Spanish
References: 27
Page: 171-178
PDF size: 520.87 Kb.


ABSTRACT

Introduction: Studies related to DNA analysis have set a standard for development in the basic sciences, and one of the requirements for obtaining good results is the quality of the genetic material extracted, together with the method used for the samples preservation. Objective: To compare three methods of preserving biopsies obtained by colonoscopy in patients with colorectal cancer, for purposes of use in molecular biology studies. Materials and methods: Biopsies by colonoscopy from nine patients with clinical diagnosis of colon cancer were taken and preserved in saline solution and two nucleic acids stabilizing solvents, RNAlater® y LifeGuard™ Soil Preservation Solution. The extraction of total DNA was carried out to the nine samples and the concentration and quality of the extracted DNA was verified. Results: DNA extraction at 24 hours, eight days, 15 days, one month, six months, one year and two years after sampling showed that the DNA from biopsies preserved in saline solution had low concentration and degraded at eight days. DNA from samples preserved in commercial stabilizing solutions had high concentration and good quality. This last one was verified by amplification of a DNA fragment associated to the APC gene by polymerase chain reaction. Conclusions: The LifeGuard™ solution as well as the RNAlater® can be used in transport and conservation of human tissues, and may be recommended for laboratories wishing to preserve specimens with different methods to paraffinembedded specimen or that do not have highly efficient conservation methods such as cryogenics.


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