2014, Number 09-10
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Medicina & Laboratorio 2014; 20 (09-10)
Enzymatic histochemical in the diagnosis of myopathies: a systematic review
Rosero-Salazar DH, Salazar-Monsalve L, Tovar-Sánchez MA
Language: Spanish
References: 64
Page: 467-488
PDF size: 1351.75 Kb.
ABSTRACT
Introduction: The injuries on skeletal muscle that go beyond their capacity to adaptation result in
characteristic pathological changes of the myopathies. In Colombia for their diagnosis is usually used
the hematoxylin-eosin stain, however, the information about many changes in muscle tissue is limited.
That is why it is necessary to carry out certain enzymatic histochemical staining according to each
myopathy.
Objective: To describe the different criteria to diagnose myopathies by histochemical techniques.
Material and methods: A systematic review was conducted which focused on search of all articles
written in English and Spanish indexed in ISI Web of Science database, Ovid Medline, PubMed,
ScienceDirect, and EBSCO. The MeSH descriptors used for the search were «histochemistry», «skeletal
muscle», «structural and congenital myopathies», «fast twitch fiber type», «slow twitch fiber type»,
and «NADH tetrazolium reductase». The terms «clinical application» and «diagnosis» were associated
to the search. All original articles, case reports, and reviews published during 1982-2014, which imple-
mented histochemical techniques to the diagnosis of myopathy or to biomedical research were included.
Results: From systematic search, 64 publications (including two books) were representative for the
drafting of this manuscript. The article was supplemented with images from muscle biopsies processed
by the Laboratory of Histology, Universidad del Valle, Colombia.
Conclusions: Standardization of
protocols of histochemical techniques, together with pathologic criteria, can guide the diagnosis of myopathies
in a timely manner and at low-cost compared with other available techniques.
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