2014, Número 09-10
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Medicina & Laboratorio 2014; 20 (09-10)
Histoquímica enzimática en el diagnóstico de miopatías: revisión sistemática
Rosero-Salazar DH, Salazar-Monsalve L, Tovar-Sánchez MA
Idioma: Español
Referencias bibliográficas: 64
Paginas: 467-488
Archivo PDF: 1351.75 Kb.
RESUMEN
Introducción: Las enfermedades del músculo esquelético en las que la lesión supera la capacidad de
adaptación conducen a cambios patológicos propios de las miopatías. En Colombia el diagnóstico se
realiza mediante tinción con hematoxilina-eosina; sin embargo, la información brindada sobre algunos
de los cambios del tejido muscular es limitada, por lo que es necesario realizar algunas técnicas de histoquímica
enzimática según la alteración.
Objetivo: Describir los criterios de tinción de diferentes técnicas
histoquímicas en el diagnóstico de las miopatías.
Materiales y métodos: Se realizó una revisión
sistemática mediante la búsqueda de artículos en inglés y español indexados en las bases de ISI Web of
Science, Ovid Medline, PubMed, ScienceDirect y EBSCO. Los descriptores MeSH utilizados fueron:
«histochemistry», «skeletal muscle», «structural and congenital myopathies», «fiber type fast twitch»,
«fiber type slow twitch» y «NADH tetrazolium reductase». Se asociaron a la búsqueda los términos
«clinical application» y «diagnosis». Se seleccionaron los artículos originales, reportes de casos y revisiones
publicadas entre 1982 y 2014 que aplicaran las técnicas de histoquímica en el diagnóstico de una
miopatía o en la investigación biomédica.
Resultados: Se seleccionaron 64 publicaciones (incluidos
dos libros) que fueron representativos para la redacción de este manuscrito, el cual fue complementado
con imágenes de biopsias musculares procesadas en el Laboratorio de Histología de la Universidad del
Valle, Colombia.
Conclusiones: Un protocolo estandarizado con técnicas de histoquímica convencional
y enzimática, asociado a los criterios patológicos, permite orientar el diagnóstico de las miopatías
oportunamente y a un menor costo respecto a otras técnicas disponibles.
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