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2019, Number 3

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Rev Cubana Hematol Inmunol Hemoter 2019; 35 (3)

Introduction of molecular genotypingof plateles antigens

Romero DY, Soler NG, Bencomo HA

Language: Spanish
References: 19
Page: 1-9
PDF size: 361.41 Kb.


ABSTRACT

Introduction: Human platelet antigens (HPA) are expressed in 6 different platelet glycoproteins. It has been described that these antigens can stimulate the production of alloantibodies once exposed to human platelets with different HPA, which causes clinical complications such as neonatal alloimmune thrombocytopenia and postransfusional purpura. Methods: The study was performed on 11 samples of patients awaiting kidney transplantation of genotype of the HPA-1,2,3 a/b antigens by multiplex PCR, while for the genotype study of the HPA-5a/b antigens was used the PCR technique with primer-specificsequence. The amplified DNA products were visualized by agarose gel electrophoresis and by capillary electrophoresis. Results: The analysis of DNA fragments amplified by agarose electrophoresis and capillary electrophoresis revealed similar results in both methods. For the HPA-1, -2 antigens, 63% of the samples were homozygous for phenotype (a) while heterozygosity was observed in all cases for the HPA-3 genotype. In the HPA-5 system, 54% were homozygous for the phenotype (a) and 46% were heterozygous. For the genotype of HPA-15, 4% were homozygous for phenotype (b) while 96% proved heterozygous. Conclusions: These results show similarities for the genotypes HPA 1, 2.3 a/b, HPA 5a/b and HPA15 a/bwith respect to report in literature.


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