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ISSN 0210-5187 (Electronic)

2020, Number 1

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Med Cutan Iber Lat Am 2020; 48 (1)

Next generation sequencing: use in dermatology

Pinedo-Donelli S, Ball E

Language: Spanish
References: 59
Page: 47-62
PDF size: 682.07 Kb.


ABSTRACT

In 2001 the first draft of the human genome was created using a method called Sanger sequencing, which is based on the identification of the order or sequence of the nitrogenous bases within the DNA molecule. Because it was a complicated method, the genome sequencing took approximately 10 years and the cost was very high. This led to researchers developing new techniques that made it possible to know the nature of the DNA molecule of multiple genes and organisms quickly and at a lower cost. Its purpose was to be able to determine the genetic alterations or mutations that give rise to diseases and allow to know the identity of a certain organism through its genome. For example, in the case of epidemics that are caused by an unknown agent, deciphering the sequence of the nucleotides that integrate their DNA could reveal their identity, when comparing the data obtained with a reference genome. This is how new generation sequencing emerges as a method that manages to sequence multiple DNA fragments in real time and simultaneously in order to decipher the genome of a given organism or to sequence the genes responsible for a disease in a very short time. There are many uses of this technique in dermatology, for example, to choose the most appropriate treatment, predict the prognosis and therapeutic response in malignant and inflammatory skin diseases, among many others.


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