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Medicina & Laboratorio

2012, Number 09-10

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Medicina & Laboratorio 2012; 18 (09-10)

Update on dengue diagnosis: techniques evolution and their clinical application

Gutiérrez RL, Quintero GDC, Martínez GM

Language: Spanish
References: 131
Page: 411-441
PDF size: 1266.11 Kb.


ABSTRACT

Dengue is the vector-borne viral disease of major importance in the world. Because so far there is no licensed vaccine that can be used to prevent the infection and there is no specific antiviral treatment, the early and specific diagnosis is a powerful tool to provide prompt and timely management of the patient. Despite during more than six decades there has been available a wide variety of laboratory techniques that can be used for the diagnosis of dengue, there is often lack of a consensus among the people or entities responsible of the diagnosis about the advantages and disadvantages of these techniques; for this reason, the diagnosis of dengue is more difficult. Moreover, taking into account that most of the techniques used are based on the immune response against dengue virus, and a cross reactivity with other viruses genetically related could be possible; the possibility of false positives is high. Accordingly, in recent years the development and validation of molecular techniques has increased, as they are more sensitive, specific and rapid than the cellular and immunological techniques. Therefore, it is necessary to compare the usefulness of laboratory techniques to dengue diagnosis, such as cellular, immunological and molecular tests.


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