Landa-Solis C, Olivos-Meza A, Umanzour H, Cárdenas-Soria VH, Ibarra C

Language: Spanish
References: 20
Page: 47-52
PDF size: 759.46 Kb.

ABSTRACT

Mesenchymal Stem Cells (MSCs) has been studied as a suitable source of cells for tissue engineering and to treat different diseases. At present, Bone Marrow (BM) has been consid-ered the main source to obtained MSC. However, is a painful procedure that requires to be done in the operating room under anesthesia with their inherent risks and costs. Peripheral blood (PB) after mobilization is an ambulatory procedure with lower risks that has been con-sidered a novel source of MSC. However, there is no description in the literature about the isolation of MSC from PB without growth factors administration. Objectives: To isolate MSC from human peripheral blood of the remnants in the blood bags of conventional donation & characterize the MSC by flow cytometry. Materials: The remnant of 6 samples of peripheral blood of adult donors were used. Mononuclear cells were isolated by ficoll gradient, then cells were cultured in standard conditions during 14 days. Their immunophenotype was assessed by flow cytometry. Results: In the beginning of culture a population of 8% of the cells were positive to CD73, CD90 & CD105 markers. However, two different groups of cells were identified: hematopoietic & mesenchymal. At day-14 of culture hematopoietic markers decreased while mesenchymal markers increased through the time. Conclusions: Is possible to isolate cells with MSC positive markers from remnants of Human Peripheral Blood with the capability to multiplicate in standard conditions.

REFERENCES

1. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in mon-olayer cultures of guinea-pig bone marrow and spleen cells. Cell and tissue kinetics 1970;3:393-403.

2. Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol 2000;109:235-42.

3. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 2001;7:211-28.

4. Landa-Solis C, Vazquez-Maya L, Martinez-Pardo ME, et al. Use of irradiated human am-nion as a matrix for limbal stem cell culture. Cell Tissue Bank 2013;14:77-84.

5. Noort WA, Kruisselbrink AB, in't Anker PS, et al. Mesenchymal stem cells promote engraft-ment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice. Exp Hematol 2002;30:870-8.

6. Campagnoli C, Roberts I, Kumar S, Bennett PR, Fisk NM. Clonal culture of fetal cells from maternal blood. Lancet 2001;357:962.

7. Rammal H, Beroud J, Gentils M, et al. Revers-ing charges or how to improve Wharton's jelly mesenchymal stem cells culture on polyelec-trolyte multilayer films. Bio-medical materials and engineering 2013;23:299-309.

8. Tondreau T, Meuleman N, Delforge A, et al. Mesenchymal stem cells derived from CD133-positive cells in mobilized peripheral blood and cord blood: proliferation, Oct4 expression, and plasticity. Stem Cells 2005;23:1105-12.

9. Villaron EM, Almeida J, Lopez-Holgado N, et al. Mesenchymal stem cells are present in pe-ripheral blood and can engraft after allogeneic hematopoietic stem cell transplantation. Hae-matologica 2004;89:1421-7.

10. Gallardo F, Esteller M, Pujol RM, Costa C, Estrach T, Servitje O. Methylation status of the p15, p16 and MGMT promoter genes in prima-ry cutaneous T-cell lymphomas. Haematologi-ca 2004;89:1401-3.

11. Fu W-L, Zhang J-Y, Fu X, et al. Comparative study of the biological characteristics of mes-enchymal stem cells from bone marrow and peripheral blood of rats. Tissue Eng Part A 2012;18:1793-803.

12. Azouna NB, Berraeis L, Regaya Z, Jenhani F. Immunophenotyping of hematopoietic progeni-tor cells: Comparison between cord blood and adult mobilized blood grafts. World J Stem Cells 2011;3:104-12.

13. Villa-Diaz LG, Brown SE, Liu Y, et al. Deriva-tion of mesenchymal stem cells from human induced pluripotent stem cells cultured on syn-thetic substrates. Stem cells (Dayton, Ohio) 2012;30:1174-81.

14. Jin P, Wang E, Ren J, et al. Differentiation of two types of mobilized peripheral blood stem cells by microRNA and cDNA expression anal-ysis. J Transl Med 2008;6:39.

15. Bieback K, Kern S, Kocaomer A, Ferlik K, Bugert P. Comparing mesenchymal stromal cells from different human tissues: bone mar-row, adipose tissue and umbilical cord blood. Biomed Mater Eng 2008;18:S71-6.

16. Lu R-N, Miao K-R, Zhang R, et al. Haploidenti-cal hematopoietic stem cell transplantation fol-lowing myeloablative conditioning regimens in hematologic diseases with G-CSF-mobilized peripheral blood stem cells grafts without T cell depletion: a single center report of 38 cases. Med Oncol 2014;31:81.

17. Deng J, Zou ZM, Zhou TL, et al. Bone marrow mesenchymal stem cells can be mobilized into peripheral blood by G-CSF in vivo and inte-grate into traumatically injured cerebral tissue. Neurol Sci 2011;32:641-51.

18. Dominici M, Le Blanc K, Mueller I, et al. Mini-mal criteria for defining multipotent mesenchy-mal stromal cells. The International Society for Cellular Therapy position statement. Cytother-apy 2006;8:315-7.

19. Lyahyai J, Mediano DR, Ranera B, et al. Isola-tion and characterization of ovine mesenchy-mal stem cells derived from peripheral blood. BMC Vet Res 2012;8:169.

20. Jia LY, Zhang Q, Fang N, et al. [Enrichment and Biological Characteristics of Peripheral Blood-derived Mesenchymal Stem Cells in Rats]. Zhongguo Shi Yan Xue Ye Xue Za Zhi 2015;23:506-11.