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Organo Oficial de la Secretaria de Salud de Quintana Roo

2018, Number 38

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Salud Quintana Roo 2018; 11 (38)

Cryptic species in Ochelerotatus Taeniorhynchus by barcode disclosure

Chan-Chable RJ, Martínez-Arce A, Mis-Avila PC

Language: Spanish
References: 31
Page: 7-11
PDF size: 1107.04 Kb.


ABSTRACT

Introduction. Ochlerotatus Taeniorhynchus is a mosquito that inhabits coastal and brackish water swamps. It is widely distributed in the Americas. This wide distribution may be interrupted by geographical and environmental factors that may lead to its speciation.
Objective. To compare specimens of Oc. taeniorhynchus from Mexico and the United States of America (USA) at the molecular level (DNA barcoding) to show evidence of the existence of cryptic species in this species.
Materials and method. The specimens were collected with CDC light traps baited with carbon dioxide (CO2). Molecular laboratory work was carried out using standardized protocols. The MEGA program for the calculation of genetic distances and the Automatic Barcode Gap Discovery (ABGD) method were used to delimit the molecular operational taxonomic units (MOTU´s).
Results. The Kimura 2 parameter genetic distance analysis and the Neighbor-Joining tree showed two well-differentiated clades of Oc. taeniorhynchus with an average divergence value between clades greater than 2% (3.1%). On the one hand, the sequences of the specimens from Mexico were grouped and on the other hand, the sequences of specimens from the USA. This was also supported by the ABGD analysis.
Conclusion. The average genetic divergence value of 3.1% observed between the specimens of Oc. taeniorhynchus from Mexico and USA indicates that they are cryptic species. It is recommended to add ecological data and make a fine comparison of the morphology of the larvae, the exocorion of eggs and the genitalia of adult males to find the differences that separate them as species.


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