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2018, Number 3

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Rev Cubana Med Trop 2018; 70 (3)

Acanthamoeba spp. and Naegleria spp. isolated from Pamplonita river, metropolitan area of Cucuta, Colombia

Duque NVA, Gelvis CVA, Ríos RYK

Language: Spanish
References: 22
Page: 50-60
PDF size: 296.98 Kb.


ABSTRACT

Introduction: Acanthamoeba and Naegleria are genus of free-living amoeba (AVL) resistant to extreme changes in temperature and pH, isolated from different environments (soil, air and water). Due to the number of inhabitants that benefit from the waters of Pamplonita River, the lack of knowledge about its presence in the region and the increase of diseases related to water consumption, it is necessary to carry out studies on its distribution.
Objective: To identify Acanthamoeba spp and Naegleria spp in the water of the main course of Pamplonita River, in the metropolitan area of Cúcuta, Colombia.
Methods: The search for the amoebas of interest was performed by direct examination and culture at 28, 37 and 42 °C on non-nutritive agar from 21 water samples from seven sectors of Pamplonita River. Additionally, pH and temperature were measured in situ and the coliforms´ load was also determined.
Results: It was found that 76.2 % of the samples were positive for some of the amoebas, with Acanthamoeba being the most frequently isolated. 28.6 % of the cultures incubated at 42 °C were positive mainly for Naegleria spp, indicating that these thermotolerant isolates could have pathogen potential.
Conclusions: Acanthamoeba spp and Naegleria spp are frequent in waters of Pamplonita River which are areas of important human intervention. The finding of thermotolerant amoebas warns about the health risk for the population that is exposed to this water source.


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