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2019, Number 4

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Gac Med Mex 2019; 155 (4)

Detección y genotipificación de Pneumocystis jirovecii en muestras de pacientes mexicanos VIH positivos y negativos

Alcántara-Mojica A, Delhaes L, Ramírez-Corona F, Sánchez-Paredes E, Córdova-Martínez É, Morales-Villarreal FR, Sierra-Galván S, Hernández-Hernández F

Language: Spanish
References: 32
Page: 377-385
PDF size: 246.42 Kb.


ABSTRACT

Introduction: Pneumocystis jirovecii is an atypical fungus particularly detected in HIV-positive or transplant patients. Objective: To detect and genotype Pneumocystis jirovecii in patient samples from two hospitals in Mexico City. Method: Eighty- nine respiratory tract samples, corresponding to 53 patients (30 HIV-positive and 23 HIV-negative) with respiratory symptoms and to 11 healthy individuals included as negative control, were processed. DNA was extracted from the ITS region and amplified by nested polymerase chain reaction from the internal transcribed spacer, with one fragment being obtained at each round (693 and 550 bp). Genotypes and their phylogenetic relationship were determined by sequencing the 550 bp fragment. Results: Forty-eight samples from 30 HIV-positive patients were received from a single hospital, out of which 11 (36.6 %) were positive for Pneumocystis jirovecii. No sample was positive in HIV-negative patients or healthy subjects. The most frequently detected haplotypes were Eg and Em. Conclusions: The frequency of Pneumocystis jirovecii infection was high in the studied Mexican population. The most common genotype was different from those reported in other countries. It is necessary to address this health problem through early detection of this infection.


REFERENCES

  1. Wang RJ, Miller RF, Huang L. Approach to fungal infections in human immunodeficiency virus-infected individuals: Pneumocystis and beyond. Clin Chest Med. 2017;38:465-477.

  2. Calderón-Sandubete E, De Armas-Rodríguez Y, Capó-De Paz V. Pneumocystis jirovecii: cien años de historia. Rev Cubana Med Trop. 2011;63:97-116.

  3. Vera A, Pinochet R, Villamizar G, Cancino C, Henríquez L, Cabib C. Ventilación mecánica en pacientes VIH+ en falla respiratoria aguda. Experiencia de una Unidad de Cuidados Intensivos de reciente creación. Rev Chil Med Intensiva. 2006;21 67-72.

  4. Usha M, Rakshitha HB, Avnika J, Aneesha A, Sharon R. Morphological spectrum of fungal infections: a retrospective study. Int J Med Sci Public Health. 2016;5:1673-1677.

  5. López-Martínez R, Méndez-Tovar LJ, Hernández-Hernández F, Castañón- Olivares LR. Micología médica. México: Trillas; 2012.

  6. Durand-Joly I, Chabé M, Soula F, Delhaes L, Camus D, Dei-Cas E. Molecular diagnosis of pneumocystis pneumonia. FEMS Immunol Med Microbiol. 2005;45:405-410.

  7. Das S, Deb B. DNA barcoding of fungi using ribosomal ITS marker for genetic diversity analysis: a review. Int J Pure App Biosci. 2015;3:160-167.

  8. Rodríguez-Tovar A, Xoconostle-Cázares B, Valdés M. Ecología molecular de los hongos ectomicorrízicos. Rev Fitotec Mex. 2004;27:267-278.

  9. Lee CH, Helweg-Larsen J, Tang X, Jin S, Li B, Bartlett MS, et al. Update on Pneumocystis carinii f. sp. hominis typing based on nucleotide sequence variations in internal transcribed spacer regions of rRNA genes. J Clin Microbiol. 1998;36:734-741.

  10. Miller RF, Lindley AR, Ambrose HE, Malin AS, Wakefield AE. Genotypes of Pneumocystis jirovecii isolates obtained in Harare, Zimbabwe, and London, United Kingdom. Antimicrob Agents Chemother. 2003;47:3979-3981.

  11. Wissmann G, Morilla R, Friaza V, Calderón E, Varela JM. El ser humano como reservorio de Pneumocystis. Enferm Infecc Microbiol Clin. 2010;28:38-43.

  12. Schmiedel Y, Zimmerli S. Common invasive fungal diseases: an overview of invasive candidiasis, aspergillosis, cryptococcosis, and Pneumocystis pneumonia. Swiss Med Wkly. 2016;146:w14281.

  13. Casanova-Cardiel LJ, Barriga-Angulo G, Ruiz-Ordaz I, Fuentes-Allen JL. Frecuencia de Pneumocystis carinii en 128 pacientes con síndrome de inmunodeficiencia adquirida y neumonía intersticial. Diagnóstico mediante expectoración inducida. Rev Med Inst Mex Seg Soc. 1992;30:191-194.

  14. García-Sancho MC, Pérez-González LE, Franco-Marina F, Reyes-Terán G. Infecciones oportunistas pulmonares en pacientes con infección por el virus de la inmunodeficiencia humana del Instituto Nacional de Enfermedades Respiratorias 1991-2001. Rev Inst Nal Enf Resp Mex. 2003;16:6-10.

  15. Lu JJ, Bartlett MS, Shaw MM, Queener SF, Smith JW, Ortiz-Rivera M, et al. Typing of pneumocystis carinii strains that infect humans base on nucleotide sequence variations of internal transcribed spacers of rRNA genes. J Clin Microbiol. 1994;32:2904-2912.

  16. Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977;74 5463-5467.

  17. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. Mega 6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol. 2013;30:2725-2729.

  18. Lu JJ, Chen CH, Bartlett MS, Smith JW, Lee CH. Comparison of six different PCR methods for detection of Pneumocystis carinii. J Clin Microbiol. 1995;33:2785-2788.

  19. Atzori C, Angeli E, Agostoni F, Mainini A, Micheli V, Cargnel A. Biomolecular techniques to detect Pneumocystis carinii f. sp. hominis pneumonia in patients with acquired inmunodeficiency syndrome. Int J Infect Dis. 1998-1999;3:76-81.

  20. Álvarez-Martínez MJ, Miró JM, Valls ME, Moreno A, Rivas PV, Solé M, et al. Sensitivity and specificity of nested and real-time PCR in the detection of Pneumocystis jirovecii in clinical specimens. Diagn Microbiol Infect Dis. 2006;56:153-160.

  21. Gupta R, Mirdha BR, Guleria R, Mohan A, Kabra SK, Kumar L, et al. Use of different primer directed sequence amplification by polymerase chain reaction for identification of Pneumocystis jirovecii in clinical samples. Indian J Chest Dis Allied Sci. 2008;50:321-327.

  22. Pereira RM, Müller AL, Zimerman RA, Antunes DB, Zinn VF, Friaza V, et al. High prevalence of Pneumocystis jirovecii colonization among HIV-positive patients in southern Brazil. Med Mycol. 2014;52:804-809.

  23. Lundgren B, Wakefield AE. PCR for detecting Pneumocystis carinii in clinical or environmental samples. FEMS Immunol Med Microbiol. 1998;22:97-101.

  24. Sheikholeslami MF, Sadraei J, Farnia P, Forozandeh-Moghadam M, Emadi-Kochak H. Co-infection of Mycobacterium tuberculosis and Pneumocystis jirovecii in the Iranian patients with human immunodeficiency virus. Jundishapur J Microbiol. 2015;8:e17254.

  25. Medrano FJ, Montes-Cano MA, Conde M, De la Horra C, Respaldiza N, Gasch A, et al. Pneumocystis jirovecii in general population. Emerg Infect Dis. 2005;11:245-250.

  26. Vargas SL, Hughes WT, Santolaya ME, Ulloa AV, Ponce CA, Cabrera CE, et al. Search for primary infection by Pneumocystis carinii in a cohort of normal healthy infants. Clin Infect Dis. 2001;32:855-861.

  27. Skolnik K, Huston S, Mody CH. Cryptococcal lung infections. Clin Chest Med. 2017;38:451-464.

  28. Tsolaki AG, Miller RF, Underwood AP, Banerji S, Wakefield AE. Genetic diversity at the internal transcribed spacer regions of the rRNA operon among isolates of Pneumocystis carinii from AIDS patients with recurrent pneumonia. J Infect Dis. 1996;174:141-156.

  29. Sokulska M, Kicia M, Wesolowska M, Piesiak P, Kowal A, Lobo ML, et al. Genotyping of Pneumocystis jirovecii in colonized patients with various pulmonary diseases. Med Mycol. 2018;56:809-815.

  30. Beser J, Botero-Kleiven S, Lebbad M, Hagblom P, Fernandez V. A limited number of ITS haplotypes defines the diversity of Pneumocystis jirovecii strains in Sweden. Infect Genet Evol. 2011;11:948-954.

  31. Villasís-Keever A, Rangel-Frausto MS, Ruiz-Palacios G, Ponce de León-Rosales S. Clinical manifestations and survival trends during the first 12 years of the AIDS epidemic in Mexico. Arch Med Res. 2001;32:62-65.

  32. Bongomin F, Gago S, Oladele RO, Denning DW. Global and multi-national prevalence of fungal diseases-estimate precision. J Fungi (Basel). 2017;3:57.




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