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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2019, Number 2

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Rev Invest Clin 2019; 71 (2)

Fasting Insulin and Alanine Amino Transferase, but not FGF21, Were Independent Parameters Related with Irisin Increment after Intensive Aerobic Exercising

de la Torre-Saldaña VA, Gómez-Sámano MÁ, Gómez-Pérez FJ, Rosas-Saucedo J, León-Suárez A, Grajales-Gómez M, Oseguera-Moguel J, Vega- Beyhart A, Cuevas-Ramos D

Language: English
References: 36
Page: 133-140
PDF size: 213.74 Kb.


ABSTRACT

Background: Irisin is a protein cleaved from fibronectin type III domain-containing protein 5 and has been implicated in the beneficial effects of exercise. However, it is unknown which factors contribute to irisin increment after intensive exercising in humans. This study aimed to assess independent factors related with serum irisin after 2 weeks of supervised physical activity in young sedentary healthy women. Design and Methods: We developed a comparative, interventional, longitudinal, and prospective study at a third-level specialty health center. Between March 2010 and August 2011, 82 sedentary young adult women, without chronic diseases or regular medical treatments, were recruited. A total of 38 women fulfilled selection criteria, and irisin concentrations were quantified before and after the intervention. Independent factors related with irisin increment were evaluated according to mild to moderate and vigorous intensity of physical activity. A supervised treadmill exercise test following the Bruce’s protocol was conducted from Monday to Friday during 2 weeks. In addition, anthropometric measurements were taken, and fibroblast growth factor 21 (FGF21), glucose, insulin, and liver transaminases were measured. Results: Intensity of exercising was directly related to irisin (p = 0.02) and FGF21 (p = 0.01) serum levels. However, an independent and significant relationship between FGF21 and irisin was not confirmed. A novel association was found between alanine aminotransferase (ALT) and irisin, showing a positive and significant correlation (r = 0.37, p = 0.02). The association was particularly strong with higher intensity of aerobic exercising (r = 0.64, p = 0.01). Linear regression model adjusted for glucose and body mass index confirmed an independent association between ALT and irisin and also between insulin and irisin (adjusted R² = 0.12, p = 0.04). Such association increased after grouping in moderate to vigorous physical activity intensity (adjusted R² = 0.46, F = 4.7, p = 0.03). Conclusions: Serum irisin and FGF21 levels significantly increased after 2 weeks of supervised physical activity. However, only fasting insulin and ALT, but not FGF21, were independent parameters explaining irisin increment, mainly after moderate to vigorous exercising.


REFERENCES

  1. Jedrychowski MP, Wrann CD, Paulo JA, et al. Detection and quantitation of circulating human irisin by tandem mass spectrometry. Cell Metab. 2015;22:734-40.

  2. Stepto NK, Benziane B, Wadley GD, et al. Short-term intensified cycle training alters acute and chronic responses of PGC1α and cytochrome C oxidase IV to exercise in human skeletal muscle. PLoS One. 2012;7:e53080.

  3. Boström P, Wu J, Jedrychowski MP, et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481:463-8.

  4. Yan Z, Okutsu M, Akhtar YN, Lira VA. Regulation of exerciseinduced fiber type transformation, mitochondrial biogenesis, and angiogenesis in skeletal muscle. J Appl Physiol (1985). 2011; 110:264-74.

  5. Cuevas-Ramos D, Almeda-Valdés P, Meza-Arana CE, et al. Exercise increases serum fibroblast growth factor 21 (FGF21) levels. PLoS One. 2012;7:e38022.

  6. Zhang X, Yeung DC, Karpisek M, et al. Serum FGF21 levels are increased in obesity and are independently associated with the metabolic syndrome in humans. Diabetes. 2008;57:1246-53.

  7. Cuevas-Ramos D, Aguilar-Salinas CA. Modulation of energy balance by fibroblast growth factor 21. Horm Mol Biol Clin Investig. 2016;30:???.

  8. Nedergaard J, Bengtsson T, Cannon B. Unexpected evidence for active brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab. 2007;293:E444-52.

  9. Ouellet V, Labbé SM, Blondin DP, et al. Brown adipose tissue oxidative metabolism contributes to energy expenditure during acute cold exposure in humans. J Clin Invest. 2012;122: 545-52.

  10. Kelly DP. Medicine. Irisin, light my fire. Science. 2012;336:42-3.

  11. Lee HJ, Lee JO, Kim N, et al. Irisin, a novel myokine, regulates glucose uptake in skeletal muscle cells via AMPK. Mol Endocrinol. 2015;29:873-81.

  12. Huh JY, Panagiotou G, Mougios V, et al. FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. MRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism. 2012; 61:1725-38.

  13. Moreno-Navarrete JM, Ortega F, Serrano M, et al. Irisin is expressed and produced by human muscle and adipose tissue in association with obesity and insulin resistance. J Clin Endocrinol Metab. 2013;98:E769-78.

  14. Crujeiras AB, Zulet MA, Lopez-Legarrea P, et al. Association between circulating irisin levels and the promotion of insulin resistance during the weight maintenance period after a dietary weight-lowering program in obese patients. Metabolism. 2014; 63:520-31.

  15. Tremblay MS, Colley RC, Saunders TJ, Healy GN, Owen N. Physiological and health implications of a sedentary lifestyle. Appl Physiol Nutr Metab. 2010;35:725-40.

  16. Hill J, Timmis A. Exercise tolerance testing. BMJ. 2002;324: 1084-7.

  17. Albrecht E, Norheim F, Thiede B, et al. Irisin-a myth rather than an exercise-inducible myokine. Sci Rep. 2015;5:8889.

  18. Lee P, Linderman JD, Smith S, et al. Irisin and FGF21 are coldinduced endocrine activators of brown fat function in humans. Cell Metab. 2014;19:302-9.

  19. Löffler D, Müller U, Scheuermann K, et al. Serum irisin levels are regulated by acute strenuous exercise. J Clin Endocrinol Metab. 2015;100:1289-99.

  20. Huh JY, Siopi A, Mougios V, Park KH, Mantzoros CS. Irisin in response to exercise in humans with and without metabolic syndrome. J Clin Endocrinol Metab. 2015;100:E453-7.

  21. Daskalopoulou SS, Cooke AB, Gomez YH, et al. Plasma irisin levels progressively increase in response to increasing exercise workloads in young, healthy, active subjects. Eur J Endocrinol. 2014;171:343-52.

  22. Tsuchiya Y, Mizuno S, Goto K. Irisin response to downhill running exercise in humans. J Exerc Nutrition Biochem. 2018;22:12-7.

  23. Korkmaz A, Venojärvi M, Wasenius N, et al. Plasma irisin is increased following 12 weeks of nordic walking and associates with glucose homoeostasis in overweight/obese men with impaired glucose regulation. Eur J Sport Sci. 2018;???:1-9.

  24. Son JS, Chae SA, Testroet ED, Du M, Jun HP. Exercise-induced myokines: a brief review of controversial issues of this decade. Expert Rev Endocrinol Metab. 2018;13:51-8.

  25. Kim KH, Kim SH, Min YK, et al. Acute exercise induces FGF21 expression in mice and in healthy humans. PLoS One. 2013; 8:e63517.

  26. Fletcher JA, Linden MA, Sheldon RD, et al. Fibroblast growth factor 21 and exercise-induced hepatic mitochondrial adaptations. Am J Physiol Gastrointest Liver Physiol. 2016;310:G832-43.

  27. Cuevas-Ramos D, Almeda-Valdes P, Gómez-Pérez FJ, et al. Daily physical activity, fasting glucose, uric acid, and body mass index are independent factors associated with serum fibroblast growth factor 21 levels. Eur J Endocrinol. 2010;163:469-77.

  28. Kharitonenkov A, Shiyanova TL, Koester A, et al. FGF-21 as a novel metabolic regulator. J Clin Invest. 2005;115:1627-35.

  29. Zhang Y, Li R, Meng Y, et al. Irisin stimulates browning of white adipocytes through mitogen-activated protein kinase p38 MAP kinase and ERK MAP kinase signaling. Diabetes. 2014;63: 514-25.

  30. Choi YK, Kim MK, Bae KH, et al. Serum irisin levels in new-onset Type 2 diabetes. Diabetes Res Clin Pract. 2013;100:96-101.

  31. Liu TY, Shi CX, Gao R, et al. Irisin inhibits hepatic gluconeogenesis and increases glycogen synthesis via the PI3K/Akt pathway in Type 2 diabetic mice and hepatocytes. Clin Sci (Lond). 2015; 129:839-50.

  32. Zhang HJ, Zhang XF, Ma ZM, et al. Irisin is inversely associated with intrahepatic triglyceride contents in obese adults. J Hepatol. 2013;59:557-62.

  33. Park MJ, Kim DI, Choi JH, Heo YR, Park SH. New role of irisin in hepatocytes: the protective effect of hepatic steatosis in vitro. Cell Signal. 2015;27:1831-9.

  34. Dushay J, Chui PC, Gopalakrishnan GS, et al. Increased fibroblast growth factor 21 in obesity and nonalcoholic fatty liver disease. Gastroenterology. 2010;139:456-63.

  35. Li H, Fang Q, Gao F, et al. Fibroblast growth factor 21 levels are increased in nonalcoholic fatty liver disease patients and are correlated with hepatic triglyceride. J Hepatol. 2010;53:934-40.

  36. Yilmaz Y, Eren F, Yonal O, et al. Increased serum FGF21 levels in patients with nonalcoholic fatty liver disease. Eur J Clin Invest. 2010;40:887-92.




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