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ISSN 0864-4551 (Print)

2018, Number 1

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Biotecnol Apl 2018; 35 (1)

Identification and characterization of a Beauveria bassiana (Bals.) Vuill. isolate having a high potential for the control of the Diatraea sp. sugarcane stem borer

García JL, Sotelo P, Monroy DM, Barrera G, Gómez-Valderrama J, Espinel C, Barreto E, Villamizar LF

Language: English
References: 30
Page: 1201-1207
PDF size: 556.85 Kb.


ABSTRACT

A native isolate of the Beauveria sp. fungus obtained from the Diatraea sp. larva was studied and identified as B. bassiana by analyses based on ribosomal internal transcribed spacer (ITS) sequences. The code given to the isolate was Bv062 and its insecticidal activity was evaluated on the second instar larvae of D. saccharalis, D. indigenella, D. tabernella and D. busckella, under laboratory conditions, producing mortality rates of 73.3, 83.3, 67.6 and 24.1 %, respectively. Subsequently, the activity of the fungus was evaluated on D. saccharalis eggs. Egg mortality was of 82.2 % when spraying a conidial suspension adjusted to a concentration of 1 × 108 conidia/mL on the eggs. The mean lethal concentration (LC50) was found to be 9.04 × 105 conidia/ mL and the lethal concentration ninety (LC90) was of 2.07 × 107 conidia/mL on the second instar larvae of D. saccharalis. In-plant bioassays under semi-controlled conditions showed that fungus efficacy was above 50 % with the sporulation of dead larvae of 80 %, using concentrations between 1.0 × 107 and 5.0 × 107 conidia/ mL. Results demonstrated the high potential of B. bassiana Bv062 as a biocontrol agent for the development of a bio-insecticide to be used in the management of different species of the sugarcane stem borer complex.


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