medigraphic.com
ISSN 1561-2996 (Electronic)
ISSN 0864-0289 (Print)

2018, Number 3

<< Back Next >>

Rev Cubana Hematol Inmunol Hemoter 2018; 34 (3)

Gammagraphic estudy of the transplant of autologous mononuclear cells in patients with chronic arterial insufficiency

Hernández RLH, Fundora ST, Hernández RP, Ballet LF, Urgellés LA, Fernández CY

Language: Spanish
References: 22
Page: 1-9
PDF size: 281.79 Kb.


ABSTRACT

Introduction: Regenerative cell therapy is currently a therapeutic option to reduce the high rates of morbidity and amputation due to peripheral arterial disease. On the other hand, radiotracer imaging techniques are a valuable tool to evaluate the efficacy and safety of this novel therapeutic modality.
Objective: To evaluate the efficiency of intraarterial transplantation of peripheral blood mononuclear cells (CMN-SP) in a group of patients with stage IIb chronic arterial insufficiency by means of the gammagraphic study.
Method: We studied 10 patients with an average age of 60 years and chronic arterial insufficiency in stage IIB who were included in a randomized, single longitudinal, randomized prospective study, during the period between October 2012 and October 2014. The scintigraphic study was performed before and three months after the regenerative cell therapy.
Results: 70 % of the studied cases showed an increase in the percentage of total radiotracer counts in the anatomical area that was studied and only 30% of them did not show variations of the percentage of total radionuclide counts in the region of interest.
Conclusions: The increase of the gammagraphic variable on study showed the effectiveness of the regenerative cellular treatment in the majority of the treated patients. We suggest enhancing the use of imaging techniques with radiotracers in the evaluation studies of the efficacy of regenerative cell therapy in patients with peripheral arterial disease.


REFERENCES

  1. Stacy MR, Zhou W, Sinusas AJ. Radiotracer Imaging of Peripheral Vascular Disease. J Nucl Med Technol. 2015;43(3):185-92.

  2. Feito Castex T, Perurena Llamosa C, Aparicio Suárez JL, Bustillo Santandreu MJ, García Seco F. Implante de células madre hematopoyéticas en pacientes con isquemia crónica de los miembros inferiores. Rev Cubana Angiol Cir Vasc.2015;16 (1):64-75.

  3. Hernández Ramírez P, Artaza Sánz H, Aparicio Suárez JL, Cruz Tamayo F, Díaz Díaz AJ, Fernández Delgado ND, et al. Impacto de la medicina regenerativa en angiología. Experiencia cubana. Rev Cubana Angiol Cir Vasc [Internet]. 2017 Jun [citado 2017 Ago 31];18(1):3-18. Disponible en: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1682- 00372017000100002&lng=es

  4. Davies M. Critical limb ischemia:cell and molecular therapies for limb salvage. Methodist Debakey Cardiovasc J. 2013;9(2):69-72.

  5. Bencomo Hernández A. Los derivados plaquetarios en medicina regenerativa. Rev Cubana Hematol Inmunol Hemoter. 2012;28(4):308-9.

  6. Fernández Delgado ND, Hernández Ramirez P, Forrellat-Barrios M. Espectro funcional de las plaquetas: de la hemostasia a la medicina regenerativa. Rev Cubana Hematol Inmunol Hemoter. 2012;28(3):200-16.

  7. Holger L, Peter B, Berthold A. Stem cell and progenitor cell therapy in peripheral artery Disease. Thromb Haemost 2010;103:696-709.

  8. Gaertner H, Kessler H, Wester M, Schwaiger A, Beer J. Radiolabelled RGD peptides for imaging and therapy. Eur J Nucl Med Mol Imaging. 2012; 39(Suppl 1):126-38.

  9. Hernández Seara A, Cabrera Zamora JL, Viña Cisneros H, Hondares Guzmán MC, López Díaz M, Licort García E. Caracterización de los pacientes ingresados por isquemia crítica de los miembros inferiores. Rev Cubana Angiol Cir Vasc [Internet]. 2013 [citado 2017 Ago 31];14(1). Disponible en: http://bvs.sld.cu/revistas/ang/vol14_1_13/angio04113.htm .

  10. Benitez E, Brandon J, Jason Ch, Bauer E. Contemporary assessment of foot perfusion in patients with critical limb ischemia. Sem Vasc Surg. 2014;27(1):3-15.

  11. Kinlay S. Prospects for multimodality imaging in peripheral artery disease. Circ Cardiovasc Imaging. 2014 Jan;7(1):3-4. doi: 10.1161/CIRCIMAGING.113.001434.

  12. Hernández-Ramírez P, Forrellat-Barrios M. Ventajas de la terapia celular con células mononucleares derivadas de la médula ósea, aplicadas en su conjunto. Rev Cubana Hematol Inmunol Hemoter. 2013;29 (4):407-12.

  13. Hernández P, Artaza H, Díaz AJ, Cortina LD, Lam RM, Pol N, et al. Autotrasplante de células madre adultas en miembros inferiores con isquemia crítica. Rev Esp Invest Quirur 2007;10:204-11.

  14. Tara S, Miyamoto M, Takagi G, Kirinoki-Ichikawa S, Tezuka A, Hada T, et al. Low-energy extracorporeal shock wave therapy improves microcirculation blood flow of ischemic limbs in patients with peripheral arterial disease: pilot study. J Nippon Med Sch. 2014;81(1):19-27.

  15. Matthias G. Perfusion Imaging. J Magn Res. 2014;40:269-79.

  16. Hernández Reyes LH, Fundora Sarraff T, Hernández Ramírez P, Janero Valdés A. La imagen radioisotópica: una herramienta eficaz en la terapia regenerativa. Rev Cubana Hematol Inmunol Hemoter. 2017;33(2):1-9.

  17. Liu JT, Chang CS, Su CH, Li CS. Insights Into Microcirculation Underlying Critical Limb Ischemia by Single-Photon Emission Computed Tomography. Medicine (Baltimore). 2015 Jul;94(27):e1075. doi: 10.1097/MD.0000000000001075.

  18. Takagi G, Miyamoto M, Fukushima Y, Yasutake M, Tara S, Takagi I, et al. Imaging Angiogenesis Using 99mTc-Macroaggregated Albumin Scintigraphy in Patients with Peripheral Artery Disease. J Nucl Med. 2016 Feb;57(2):192-7. doi: 10.2967/jnumed.115.160937.

  19. Lasala P, Silva A, Gardner A, Minguell J. Combination Stem Cell Therapy for the Treatment of Severe Limb Ischemia: Safety and Efficacy Analysis. Angiology. 2010;61(6):551-6.

  20. Shuhei T, Masaaki M, Gen T, Yoshimitsu F, Sonoko K. Prediction of Limb Salvage after Therapeutic Angiogenesis by Autologous Bone Marrow Cell Implantation in Patients with Critical Limb Ischemia. Ann Vasc Dis. 2011;.4(1):24-31.

  21. Solanki K, Dondi M. Radiolabelled autologous cells: methods and standardization for clinical use. 5ta ed. VIENNA: Sales Unit, Publishing Section International Atomic Energy Agency; 2015. p. 7-8.

  22. Lemonitsa H, Henk M, Verheul W, Harry H, Maqsood Y, Adriaan A, Willemien M, Houven van O. Molecular imaging of targeted therapies with positron emission tomography: the visualization of personalized cancer care. Cell Oncol. 2015;38:49-64.




2020     |     www.medigraphic.com