medigraphic.com
ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2018, Number 3

<< Back Next >>

Rev Mex Periodontol 2018; 9 (3)

Neutrophils and epithelial cells in saliva swabs of patients with chronic periodontitis: pilot study

Ortiz-García YM, García-Iglesias T, García OA, Montaño-Cisneros IM, López-Tela DL, Zamora-Pérez AL

Language: Spanish
References: 19
Page: 45-49
PDF size: 261.33 Kb.


ABSTRACT

Background: Chronic periodontitis (CP) is a destructive inflammatory process caused by dysbiosis of oral microbiota, which results in an altered bone metabolism. The permeability of the binding epithelium allows the extravasation of plasma proteins and cellular components such as neutrophils. The objective of this work is to quantify the number of neutrophils and epithelial cells in salivary smears of subjects with CP. Material and methods: This is a cross-sectional study. Two groups were formed: group 1 or CS (n = 10) and group 2 or CP (n = 10). Saliva smears were made and stained with Hemocolorante Rápido Hycel No. Cat. 548. The number of neutrophils and epithelial cells were quantified by light microscopy. Results: The number of neutrophils in saliva was significantly higher in the group of subjects with CP compared to the SC. The percentage of neutrophils in the CP group (17%) is higher than the percentage of neutrophils in the SC group (9.5%). Conclusion: Preliminary results show that salivary smears from subjects with CP show a greater number of neutrophils compared to the CS group, therefore salivary smears could be an informative source for the monitoring of the cellular component in saliva.


REFERENCES

  1. Hajishengallis G. Periodontitis: from microbial immune subversion to systemic inflammation. Nat Rev Immunol. 2015; 15: 30-44.

  2. Kinane DF, Stathopoulou PG, Papapanou PN. Periodontal diseases. Nat Rev Dis Primers. 2017; 3: 1-14.

  3. Kinane DF, Lappin DF. Immune processes in periodontal disease: a review. Ann Periodontol. 2002; 7: 62-71.

  4. Zaric SS, Lappin MJ, Fulton CR, Lundy FT, Coulter WA, Irwin CR. Sialylation of porphyromonas gingivalis LPS and its effect on bacterial-host interactions. Innate Immun. 2017; 23 (3): 319-326.

  5. Bartold PM, Narayanan AS. Molecular and cell biology of healthy and diseased periodontal tissues. Periodontol 2000. 2006; 40: 29-49.

  6. Palaska I, Gagari E, Theoharides TC. The effects of P. gingivalis and E. coli LPS on the expression of proinflammatory mediators in human mast cells and their relevance to periodontal disease. J Biol Regul Homeost Agents. 2016; 30 (3): 655-664.

  7. Farnaud SJ, Kosti O, Getting SJ, Renshaw D. Saliva: physiology and diagnostic potential in health and disease. Scientific World Journal. 2010; 16: 434-456.

  8. Nicu EA, Loos BG. Polymorphonuclear neutrophils in periodontitis and their possible modulation as a therapeutic approach. Periodontol 2000. 2016; 71 (1): 140-163.

  9. Shah R, Thomas R, Mehta DS. Neutrophil priming: Implications in periodontal disease. J Indian Soc Periodontol. 2017; 21 (3): 180-185.

  10. Rahnama M, Czupkałło L, Kozicka M, Łobacz M. Gingival crevicular fluid composition and clinical importance in gingivitis and periodontitis. Pol J Public Health. 2014; 124 (2): 96-98.

  11. Korte DL, Kinney J. Personalized medicine: an update of salivary biomarkers for periodontal diseases. Periodontol 2000. 2016; 70 (1): 26-37.

  12. Michalke B, Rossbach B, Göen T, Schäferhenrich A, Scherer G. Saliva as a matrix for human biomonitoring in occupational and environmental medicine. Int Arch Occup Environ Health. 2015; 88 (1): 1-44.

  13. Thomas P, Holland N, Bolognesi C, Kirsch-Volders M, Bonassi S, Zeiger E et al. Buccal micronucleus cytome assay. Nat Protoc. 2009; 4 (6): 825-837.

  14. Theda C, Hwang SH, Czajko A, Loke YJ, Leong P, Craig JM. Quantitation of the cellular content of saliva and buccal swab samples. Sci Rep. 2018; 28 (1): 6944.

  15. Preshaw PM, Taylor JJ. How has research into cytokine interactions and their role in driving immune responses impacted our understanding of periodontitis? J Clin Periodontol. 2011; 38 (Suppl. 11): 60-84.

  16. Ohlrich EJ, Cullinan MP, Seymour GJ. The immunopathogenesis of periodontal disease. Aust Dent J. 2009; 54 (Suppl 1): S2-10.

  17. Hajishengallis G. Immunomicrobial pathogenesis of periodontitis: keystones, pathobionts, and host response. Trends Immunol. 2014; 35 (1): 3-11.

  18. Proctor GB. The physiology of salivary secretion. Periodontol 2000. 2016; 70 (1): 11-25.

  19. Hajishengallis G, Chavakis T, Hajishengallis E, Lambris JD. Neutrophil homeostasis and inflammation: novel paradigms from studying periodontitis. J Leukoc Biol. 2015; 98 (4): 539-548.




2020     |     www.medigraphic.com