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2018, Number 4

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Rev Hosp Jua Mex 2018; 85 (4)

Modeling techniques used to distinguish the kinetics of phenylephrine-induced contraction of thoracic aorta in rats of different ages

Padilla-Pérez J, Castillo-Hernández MC, Padilla-Keymole J, Crivelli-Puga A, López-Canales ÓA, Zambrano-Padilla R

Language: Spanish
References: 36
Page: 208-221
PDF size: 661.75 Kb.


ABSTRACT

The exponential mathematical models of 2 and 3 components with 7 and 10 temporal parameters respectively (2CM,7P, 3CM,10P) were evaluated using the kinetics (time constant τ, s) of the isometric contraction (g·min -1) induced by phenylephrine (PE, 1 µM) in vitro in aortotoracic rings (AsAoTx) of rats of different ages, in terms of their best statistical merits (Fisher test *) or their physiological significance, according to the current knowledge of the three field events on vascular smooth muscle contraction (S1, S2, S3) based on the temporal parameters (i.e., τ) of its three expected phases (Φ1, Φ2, Φ3). It was experimented with 5 groups of Wistar rats by age (5G); 6 weeks old (n = 7); 8 weeks old (n = 6), 9 weeks (n = 6), 16 weeks old (n = 6) and 78 weeks old (n = 6). Four rings were averaged per rat to obtain a single modeling response. The baseline and total amplitude of response at 5G remained stable during fit modeling. 3CM,10P distinguished (* p ‹ 0.05) a transient rapid developmental force phase (Φ1Fτ = 19 ± 2 similar to Φ1Fτ 2CM,7P = 20 ± 12), another phase of moderately rapid developmental force (Φ2Fτ = 116 ± 9 ‹ Φ2Fτ 2CM,7P = 177 ± 71) and another phase of slow developmental force (Φ3Fτ = 265 ± 12) thus identifying three expected kinetic phases based on a close match with the theoretical events (S1, S2, S3) of vascular reactivity for this condition. The 3CM,10P exponential model best fitted both statistically and physiologically the theoretical meaning of the TxAo PE-induced isometric contraction response data from the 5G, identifying three «expected» functionally different phases and ranked them kinetically (Φ1Fτ ‹ Φ2Fτ ‹ Φ3Fτ) in arterial vascular smooth muscle.


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