Pérez-Espinoza M, Brambila E

Language: Spanish
References: 28
Page: 110-117
PDF size: 81.62 Kb.

ABSTRACT

In this work we describe the elaboration and evaluation of a glucose kit based in the Trinder method. Glucose kit evaluation included studies of linearity, within-run and between-day precision assays, recovery, and method comparison to determine total error of the kit. Results showed that the kit was linear up to 400 mg/dL, in addition it had an excellent precision with within-run and between-day coefficients of variation of 0.67% and 1.13%, respectively. Glucose kit was affected only by high haemoglobin and bilirubin concentrations, and high levels of ascorbic acid. Addition of several glucose amounts to the samples was 100% recovered. Method comparison studies using the hexocinase method as a reference method shown a highly correlation (r = 0.996), but, results were approximately 5 mg/dL lower in the glucose kit prepared in our laboratory. Total error in our method (5.8 mg/dL) was acceptable as compared with the maximum allowable error to glucose assays (10 mg/dL). Reference limits obtained from selected individuals were 67 to 111 mg/dL. Based in these results we state that the glucose kit prepared in our laboratory has the analytic quality to be used for plasma glucose determinations.

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