medigraphic.com

2005, Number 3

<< Back Next >>

Bioquimia 2005; 30 (3)

Role of CYP2E1 genetic polymorphism in the risk of acute pesticide intoxication

Yerena CE, Hernández-Kelly LCR, Ramírez J, Riaño ME, del Refugio LM, Fernández S, Ortega A

Language: Spanish
References: 19
Page: 68-75
PDF size: 83.71 Kb.


ABSTRACT

Xenobiotic metabolism is dramatically influenced by the presence of allelic variants in the drug metabolizing enzymes. Several studies have indicated the presence of different expression levels of CYP 2E1, an enzyme involved in the metabolism of pesticides, resulting in a differential susceptibility to the chronic exposure to these compounds. Taking into consideration the board use of these compounds in agriculture, the aim of the present study was to establish the association of the genetic polymorphism of CYP2E1 with the intoxication symptoms in a population of sugar cane fumigators. The frequency of CYP2E1 RsaI/PstI and DraI polymorphisms was determined by PCR-RFLP methods in DNA isolated from blood samples. In parallel, a questionnaire was prepared in order to detect clinic information concerning intoxication symptoms in the fumigators. Our results indicate that the mutated allele for DraI is associated with a 4 fold- increase in susceptibility to pesticide exposure. Genetic polymorphism studies are imperative in exposed population in order to prevent or reduce occupational risks.


REFERENCES

  1. Lucas D, Ferrara R, Gonzales E, Albores A, Manno M, Berthou F. Cytochrome CYP2E1 phenotyping and genotyping in the evaluation of health risks from exposure to polluted environments. Toxicol Lett 2001; 124 (1-3): 71-82.

  2. Hong JY, Yang CS. Genetic polymorphism cytocrome P450 as a biomarker of susceptibility to environmental toxicity. Environ Healt Perspect 1997; 105 (Suppl 4): 759-762.

  3. Murray R, Granner D, Mayes P, Rodwell V. Bioquímica de Harper. 14a ed. México: El Manual Moderno; 1997: 879-880.

  4. Bolt HM, Roos PH, Their R. The cytochrome P-450 isoenzyme CYP2E1 in the biological processing of industrial chemicals: consequences for occupational and environmental medicine. Int Arch Occup Environ Health 2003; 76: 174-185.

  5. Lieber CS. Cytochrome P-4502E1: Its physiological and pathological role. Physiol Rev 1997; 77: 517-544.

  6. González-Jasso E, López T, Lucas D, Berthout F, Manno M, Ortega A, et al. CYP2E1 regulation by benzene and other small organic chemicals in rat liver and peripheral lymphocytes. Toxicol Lett 2003; 144: 55-67.

  7. Mendoza-Cantú A, Castorena-Torres F, Bermúdez M, Martínez-Hernández R, Ortega A, Salinas JE, Albores A. Genotype and allele frequencies of polymorphic cytochromes P450 CYP1A2 and CYP2E1 in Mexicans. Cell Biochem Funct 2004; 22: 29-34.

  8. Organización Mundial de la Salud. Consecuencias sanitarias del empleo de plaguicidas en la agricultura. Ginebra: Organización Mundial de la Salud, 1992.

  9. Ministerio de Salud de Costa Rica. Registro de Intoxicaciones con Plaguicidas. 1997 citado el 15 de noviembre de 2002. Disponible en: http://www.netsalud.sa.cr/ms/estadist/intox.htm

  10. Durán-Nah JJ, Collí-Quintal J. Intoxicación aguda por plaguicidas. Salud Pública Mex 2000; 42: 53-55.

  11. Dirección General de Epidemiología; Secretaría de Salud. Boletín del Sistema Nacional de Vigilancia Epidemiológica 2004; sem 52: 22.

  12. Fernández M, López M, Ortiz F. Aplicación de plaguicidas. Córdoba: Ediciones Ilustres; 2002; 23: 39-46.

  13. Graham DE. The isolation of high molecular weight DNA from whole organisms of large tissues masses. Anal Biochem 1978; 85: 609-613.

  14. Promega. PCR Core Systems. Tecnical Bulletin No. 254. USA; 2001: 1-20.

  15. Hayashi S, Watanabe J, Kaname K. Genetic polymorphism in the 5´-flanking region change transcriptional regulation of the human cytochrome P4502E1 gene. J Biochem 1991; 110: 559-565.

  16. Hirvonen A, Husgafvel-Pursiainen K, Anttila S, Karjalainen A, Vainio H. The human CYP2E1 gene and lung cancer: DraI and RsaI restriction fragment length polymorphisms in a Finnish study population. Carcinogenesis 1993; 14: 85-88.

  17. Maldonado A, Buitrón R, Granda P, Gallardo L. Reporte de la investigación de los impactos de las fumigaciones en la frontera ecuatoriana. Acción ecológica. Junio 2001 citado el 13 de diciembre de 2002. Disponible en: www.accionecologica.org/.../documentos/Informes/PDF/Informe%20Fumigaciones%20 junio%202001-espanol.pdf

  18. Dreisbach R, Rebertson W. Manual de toxicología clínica. Prevención, diagnóstico y tratamiento. 6a edición. México: El Manual Moderno; 1988: 85-126.

  19. Reigart JR, Roberts JR. Reconocimiento y manejo de los envenenamientos por pesticidas. 5a edición. Washington, DC; U.S Environmental Protection Agency: 1999. p.11, 232-44. (citado el 27 de enero de 2003): Disponible en: http://www.epa.gov/oppfead1/safety/spanish/healthcare/handbook/handbook.htm




2020     |     www.medigraphic.com